BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1701 1 RBS-1\Strong range1701 1 1 15 annotation1702 1 RBS range1702 1 8 12 annotation7025 1 BBa_B0030 range7025 1 1 15 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K284033 1 BBa_K284033 finP misc_RNA from Plasmid F 2009-10-15T11:00:00Z 2015-05-08T01:11:48Z E. coli F plasmid (conjugative) Antisense RNA of tra operon; binds traJ mRNA leader and blocks translation; requires the RNA-binding protein FinO to achieve duplex formation. false false _386_ 0 4974 9 Not in stock false Finp was amplified from E. coli F plasmid (conjugative). false Gabriel Francisco Zaniboni, Marcelo Colika Bassalo, Victor Augusti Negri and Bruno Vaz de oliveira BBa_K284080 1 BBa_K284080 Upstream construction flanking FSTK gene 2009-10-16T11:00:00Z 2015-05-08T01:11:48Z Assembly from other biobricks This divice blocks conjugation by acting on the TraJ Operon. It can be excised form the genome by a Cre recombinase, which recognizes the Lox66 site. false false _386_ 0 4974 9 Not in stock false no considerations false Gabriel Francisco Zaniboni, Victor Augusti Negri, Marcelo Colika Bassalo and Bruno Vaz de oliveira component2048869 1 BBa_B0010 component2048868 1 BBa_K284033 component2048875 1 BBa_R0040 component2048866 1 BBa_B0030 component2048887 1 BBa_B0010 component2048871 1 BBa_B0012 component2048860 1 BBa_R0040 component2048889 1 BBa_B0012 component2048881 1 BBa_B0030 component2048893 1 BBa_I718016 component2048886 1 BBa_K284032 annotation2048871 1 BBa_B0012 range2048871 1 295 335 annotation2048868 1 BBa_K284033 range2048868 1 86 198 annotation2048866 1 BBa_B0030 range2048866 1 63 77 annotation2048860 1 BBa_R0040 range2048860 1 1 54 annotation2048886 1 BBa_K284032 range2048886 1 427 987 annotation2048893 1 BBa_I718016 range2048893 1 1133 1166 annotation2048889 1 BBa_B0012 range2048889 1 1084 1124 annotation2048881 1 BBa_B0030 range2048881 1 406 420 annotation2048875 1 BBa_R0040 range2048875 1 344 397 annotation2048887 1 BBa_B0010 range2048887 1 996 1075 annotation2048869 1 BBa_B0010 range2048869 1 207 286 BBa_K284032 1 BBa_K284032 finO conjugal transfer fertility inhibition protein FinO from Plasmid R100 2009-10-15T11:00:00Z 2015-05-08T01:11:48Z E. coli R100 Plasmid (conjugative) the basic protein FinO is part of the the two component FinOP system which is responsible for repressing bacterial conjugation; the FinOP system represses the transfer (tra) operon of the F-plasmid which encodes the proteins responsible for conjugative transfer of this plasmid from host to recipient Escherichia coli cells; antisense RNA, FinP is thought to interact with traJ mRNA to occlude its ribosome binding site, blocking traJ translation and thereby inhibiting transcription of the tra operon; FinO protects FinP against degradation by binding to FinP and sterically blocking the cellular endonuclease RNase E; FinO also also binds to the complementary stem-loop structures in traJ mRNA and promotes duplex formation between FinP and traJ RNA in vitro; this domain contains two independent RNA binding regions. false false _386_ 0 4974 9 Not in stock false FinO was amplified from E. coli R100 Plasmid (conjugative) false Marcelo Colika Bassalo, Gabriel Francisco Zaniboni, Victor Augusti Negri and Bruno Vaz de oliveira annotation2046231 1 stop range2046231 1 559 561 annotation2046232 1 cds range2046232 1 1 561 annotation2046230 1 start range2046230 1 1 3 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 BBa_I718016 1 lox66 lox66 2007-10-25T11:00:00Z 2015-08-31T04:07:52Z This part was generated in the form of a forward & a reverse primer. After annealing these primers EcoRI & PstI compatible cohesive ends at the 5' & 3' ends of the dsDNA were generated. Next, the dsDNA was subcloned in a pSB1A2 open plasmid (digested with EcoRI & PstI) You can follow the construction process by following the links available in the Paris iGEM 2007 wiki: http://parts.mit.edu/igem07/index.php/Paris "freezer" section plasmids table. A links sends you to the corresponding notebook date when the ligation reaction was performed lox66 is a site specific recombination cassette. It belongs to the loxP family frequently used in genetics, particularily in mouse genetics. lox site recombination is catalysed by a Site specific recombinase, Cre. lox sequences are composed of an 8 bp Core sequence surrounded by two Arms. The particularity of lox66 is that it has an altered sequence at the end of it's left arm compared to loxP. This sequence variation reduces affinity of the Cre recombinase for the arm. As a consequence, after a recombination between a lox66 and a lox71 (altered right arm sequence), one of the two resulting generated lox sites has very low recombination potential as it inherited both mutated arms. Use of lox66 & lox71 sites is potentially interresting when the recombination reaction must be "irreversible". false false _141_ 0 1568 9 In stock false No modidification was made on the lox66 sequence true Eimad Shotar BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986785 1 -35 range1986785 1 20 25 annotation1986787 1 -10 range1986787 1 43 48 annotation1986783 1 TetR 1 range1986783 1 1 19 annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986786 1 TetR 2 range1986786 1 26 44 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K284032_sequence 1 atgacagagcagaaacgaccggtactgacactgaaacggaaaacggaaggggaaacaccgacccggagccggaaaaccatcatcaatgtcaccacgccaccaaaatggaaggtgaaaaagcagaaactggcggagaaggctgcccgggaagcagagctgacagcaaaaaaagcgcaggccagacaggcgctgtccatttatctgaacctgccctcgctggatgaggccgtgaacaccctgaaaccctggtggccgggattatttgacggtgacacaccccgacttctggcctgcggtatccgggacgtgttactggaagacgtggcgcagcggaatatcccgctctcgcataaaaaactgcgcagggcgctgaaggccatcacccgttcagaaagctatctgtgtgccatgaaagccggtgcctgccggtatgacacggaagggtatgtgacggagcatatttctcaggaggaagaagtgtatgcggcagagcgtctggataaaatccgccgccagaaccggataaaggcagaacttcaggccgtgcttgatgaacaataa BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_B0030_sequence 1 attaaagaggagaaa BBa_K284080_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaatactagagtgacgagcatgtttttgttgaatacgatccatcggatacataggaacctcctcacaaaggattctatggacagtcgatgcagggagttcacgtctccctgcatcggcgatttttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaatactagatgacagagcagaaacgaccggtactgacactgaaacggaaaacggaaggggaaacaccgacccggagccggaaaaccatcatcaatgtcaccacgccaccaaaatggaaggtgaaaaagcagaaactggcggagaaggctgcccgggaagcagagctgacagcaaaaaaagcgcaggccagacaggcgctgtccatttatctgaacctgccctcgctggatgaggccgtgaacaccctgaaaccctggtggccgggattatttgacggtgacacaccccgacttctggcctgcggtatccgggacgtgttactggaagacgtggcgcagcggaatatcccgctctcgcataaaaaactgcgcagggcgctgaaggccatcacccgttcagaaagctatctgtgtgccatgaaagccggtgcctgccggtatgacacggaagggtatgtgacggagcatatttctcaggaggaagaagtgtatgcggcagagcgtctggataaaatccgccgccagaaccggataaaggcagaacttcaggccgtgcttgatgaacaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagataacttggtatagcatacattatacgaacggta BBa_I718016_sequence 1 ataacttggtatagcatacattatacgaacggta BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K284033_sequence 1 tgacgagcatgtttttgttgaatacgatccatcggatacataggaacctcctcacaaaggattctatggacagtcgatgcagggagttcacgtctccctgcatcggcgatttt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z