LOCUS BBa_K289008 7304 bp DNA linear 01-Jun-2024 DEFINITION PGBKT7(YEAST PLASMIDE BACKBONE) ACCESSION BBa_K289008 VERSION BBa_K289008.1 FEATURES Location/Qualifiers primer_bind 1155..1172 /label=dna-bd vevtor insert screening promoter 1212..1235 /label=T7 primer_bind 1469..1491 /label=dna-bd vevtor insert screening misc_feature 1342..1373 /label=T7 terminator ORIGIN 1 cggtgcgggc ctcttcgcta ttacgccaga tccttttgtt gtttccgggt gtacaatatg 61 gacttcctct tttctggcaa ccaaacccat acatcgggat tcctataata ccttcgttgg 121 tctccctaac atgtaggtgg cggaggggag atatacaata gaacagatac cagacaagac 181 ataatgggct aaacaagact acaccaatta cactgcctca ttgatggtgg tacataacga 241 actaatactg tagccctaga cttgatagcc atcatcatat cgaagtttca ctaccctttt 301 tccatttgcc atctattgaa gtaataatag gcgcatgcaa cttcttttct ttttttttct 361 tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaaa tgatggaaga 421 cactaaagga aaaaattaac gacaaagaca gcaccaacag atgtcgttgt tccagagctg 481 atgaggggta tctcgaagca cacgaaactt tttccttcct tcattcacgc acactactct 541 ctaatgagca acggtatacg gccttccttc cagttacttg aatttgaaat aaaaaaagtt 601 tgctgtcttg ctatcaagta taaatagacc tgcaattatt aatcttttgt ttcctcgtca 661 ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa gctataccaa 721 gcatacaatc aactccaagc ttgaagcaag cctcctgaaa gatgaagcta ctgtcttcta 781 tcgaacaagc atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga 841 agtgcgccaa gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaaggt 901 ctccgctgac tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc 961 tatttctact gatttttcct cgagaagacc ttgacatgat tttgaaaatg gattctttac 1021 aggatataaa agcattgtta acaggattat ttgtacaaga taatgtgaat aaagatgccg 1081 tcacagatag attggcttca gtggagactg atatgcctct aacattgaga cagcatagaa 1141 taagtgcgac atcatcatcg gaagagagta gtaacaaagg tcaaagacag ttgactgtat 1201 cgccggaatt tgtaatacga ctcactatag ggcgagccgc catcatggag gagcagaagc 1261 tgatctcaga ggaggacctg catatggcca tggaggccga attcccgggg atccgtcgac 1321 ctgcagcggc cgcataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt 1381 ttttgcgcgc ttgcagccaa gctaattccg ggcgaatttc ttatgattta tgatttttat 1441 tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt 1501 aaaacgaaaa ttcttattct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt 1561 atagcatgag gtcgctctta ttgaccacac ctctaccggc atgcaagctt ggcgtaatca 1621 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga 1681 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgaggtaact cacattaatt 1741 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga 1801 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 1861 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 1921 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 1981 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 2041 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 2101 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 2161 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 2221 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 2281 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 2341 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 2401 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 2461 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 2521 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 2581 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 2641 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 2701 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 2761 tatgagtaac ctgaggctat ggcagggcct gccgccccga cgttggctgc gagccctggg 2821 ccttcacccg aacttggggg gtggggtggg gaaaaggaag aaacgcgggc gtattggccc 2881 caatggggtc tcggtggggt atcgacagag tgccagccct gggaccgaac cccgcgttta 2941 tgaacaaacg acccaacacc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg 3001 gttccttccg gtattgtctc cttccgtgtt tcagttagcc tccccctagg gtgggcgaag 3061 aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg gaaaacgatt 3121 ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga tggcaggttg 3181 ggcgtcgctt ggtcggttca tttcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga 3241 aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag 3301 cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc 3361 tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt 3421 tccaccatga tattcggcaa gcaggcatcg tcatgggtca cgacgagatc ctcgccgtcg 3481 ggcatgctcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg 3541 tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga 3601 tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt 3661 gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc 3721 cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca 3781 gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcttgcagt 3841 tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac 3901 agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat 3961 agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatactc 4021 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 4081 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 4141 ccacctgaac gaagcatctg tgcttcattt tgtagaacaa aaatgcaacg cgagagcgct 4201 aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgaaagc 4261 gctattttac caacgaagaa tctgtgcttc atttttgtaa aacaaaaatg caacgcgaga 4321 gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg 4381 agagcgctat tttaccaaca aagaatctat acttcttttt tgttctacaa aaatgcatcc 4441 cgagagcgct atttttctaa caaagcatct tagattactt tttttctcct ttgtgcgctc 4501 tataatgcag tctcttgata actttttgca ctgtaggtcc gttaaggtta gaagaaggct 4561 actttggtgt ctattttctc ttccataaaa aaagcctgac tccacttccc gcgtttactg 4621 attactagcg aagctgcggg tgcatttttt caagataaag gcatccccga ttatattcta 4681 taccgatgtg gattgcgcat actttgtgaa cagaaagtga tagcgttgat gattcttcat 4741 tggtcagaaa attatgaacg gtttcttcta ttttgtctct atatactacg tataggaaat 4801 gtttacattt tcgtattgtt ttcgattcac tctatgaata gttcttacta caattttttt 4861 gtctaaagag taatactaga gataaacata aaaaatgtag aggtcgagtt tagatgcaag 4921 ttcaaggagc gaaaggtgga tgggtaggtt atatagggat atagcacaga gatatatagc 4981 aaagagatac ttttgagcaa tgtttgtgga agcggtattc gcaatatttt agtagctcgt 5041 tacagtccgg tgcgtttttg gttttttgaa agtgcgtctt cagagcgctt ttggttttca 5101 aaagcgctct gaagttccta tactttctag agaataggaa cttcggaata ggaacttcaa 5161 agcgtttccg aaaacgagcg cttccgaaaa tgcaacgcga gctgcgcaca tacagctcac 5221 tgttcacgtc gcacctatat ctgcgtgttg cctgtatata tatatacatg agaagaacgg 5281 catagtgcgt gtttatgctt aaatgcgtac ttatatgcgt ctatttatgt aggatgaaag 5341 gtagtctagt acctcctgtg atattatccc attccatgcg gggtatcgta tgcttccttc 5401 agcactaccc tttagctgtt ctatatgctg ccactcctca attggattag tctcatcctt 5461 caatgctatc atttcctttg atattggatc atattaagaa accattatta tcatgacatt 5521 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg 5581 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc 5641 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct 5701 taactatgcg gcatcagagc agattgtact gagagtgcac catagatcaa cgacattact 5761 atatatataa tataggaagc atttaataga acagcatcgt aatatatgtg tactttgcag 5821 ttatgacgcc agatggcagt agtggaagat attctttatt gaaaaatagc ttgtcacctt 5881 acgtacaatc ttgatccgga gcttttcttt ttttgccgat taagaattaa ttcggtcgaa 5941 aaaagaaaag gagagggcca agagggaggg cattggtgac tattgagcac gtgagtatac 6001 gtgattaagc acacaaaggc agcttggagt atgtctgtta ttaatttcac aggtagttct 6061 ggtccattgg tgaaagtttg cggcttgcag agcacagagg ccgcagaatg tgctctagat 6121 tccgatgctg acttgctggg tattatatgt gtgcccaata gaaagagaac aattgacccg 6181 gttattgcaa ggaaaatttc aagtcttgta aaagcatata aaaatagttc aggcactccg 6241 aaatacttgg ttggcgtgtt tcgtaatcaa cctaaggagg atgttttggc tctggtcaat 6301 gattacggca ttgatatcgt ccaactgcat ggagatgagt cgtggcaaga ataccaagag 6361 ttcctcggtt tgccagttat taaaagactc gtatttccaa aagactgcaa catactactc 6421 agtgcagctt cacagaaacc tcattcgttt attcccttgt ttgattcaga agcaggtggg 6481 acaggtgaac ttttggattg gaactcgatt tctgactggg ttggaaggca agagagcccc 6541 gaaagcttac attttatgtt agctggtgga ctgacgccag aaaatgttgg tgatgcgctt 6601 agattaaatg gcgttattgg tgttgatgta agcggaggtg tggagacaaa tggtgtaaaa 6661 gactctaaca aaatagcaaa tttcgtcaaa aatgctaaga aataggttat tactgagtag 6721 tatttattta agtattgttt gtgcacttgc cgatctatgc ggtgtgaaat accgcacaga 6781 tgcgtaagga gaaaataccg catcaggaaa ttgtaaacgt taatattttg ttaaaattcg 6841 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc 6901 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga 6961 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg 7021 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag 7081 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga 7141 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg 7201 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg 7261 cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg cgat //