LOCUS BBa_K300001 3748 bp DNA linear 28-May-2024 DEFINITION BioBrick integrative base vector for S. cerevisiae ACCESSION BBa_K300001 VERSION BBa_K300001.1 FEATURES Location/Qualifiers misc_feature 1..1 /label=BBa_G0012 misc_feature 2..7 /label=BBa_M31992 misc_feature 8..41 /label=BBa_J61046 (loxP) promoter 42..439 /label=TEF2 (A. gossypii)+RBS CDS 440..1249 /label=kanR(Tn903) misc_feature 715..715 /label=A->T misc_feature 982..982 /label=T->C misc_feature 1250..1514 /label=TEF2 terminator (A. gossypii) misc_feature 1515..1548 /label=BBa_J61046 (loxP) misc_feature 42..1514 /label=kanMX cassette misc_feature 8..1548 /label=BBa_K300989 misc_feature 1549..1554 /label=NheI site misc_feature 1549..1554 /label=BBa_B0045 misc_feature 1555..1599 /label=BBa_K300987 misc_feature 1600..1605 /label=NheI site misc_feature 1600..1605 /label=BBa_B0045 misc_feature 1606..1613 /label=BBa_K300990 misc_feature 1614..1619 /label=BBa_K300992 primer_bind 1677..1696 /label=VR CDS 1781..2395 /label=rep (pMB1) (rev) misc_feature 1796..1796 /label=ORI CDS 2555..3415 /label=APr (rev) misc_feature 3450..3450 /label=pBLA (rev) primer_bind 3538..3557 /label=VF2 stem_loop 3627..3646 /label=terminator misc_feature 1620..3655 /label=BBa_K300988 misc_feature 3656..3661 /label=BBa_K300993 misc_feature 3662..3669 /label=BBa_K300990 misc_feature 3670..3675 /label=AvrII site misc_feature 3670..3675 /label=BBa_B0048 misc_feature 3676..3720 /label=BBa_K300986 misc_feature 3721..3726 /label=AvrII site misc_feature 3721..3726 /label=BBa_B0048 misc_feature 3727..3732 /label=EcoRI site misc_feature 3742..3747 /label=XbaI site misc_feature 3727..3748 /label=Prefix misc_feature 3733..3740 /label=NotI site misc_feature 3727..3748 /label=BBa_G00000 ORIGIN 1 tactagtata acttcgtata atgtatgcta tacgaagtta ttaggtctag agatctgttt 61 agcttgcctc gtccccgccg ggtcacccgg ccagcgacat ggaggcccag aataccctcc 121 ttgacagtct tgacgtgcgc agctcagggg catgatgtga ctgtcgcccg tacatttagc 181 ccatacatcc ccatgtataa tcatttgcat ccatacattt tgatggccgc acggcgcgaa 241 gcaaaaatta cggctcctcg ctgcagacct gcgagcaggg aaacgctccc ctcacagacg 301 cgttgaattg tccccacgcc gcgcccctgt agagaaatat aaaaggttag gatttgccac 361 tgaggttctt ctttcatata cttcctttta aaatcttgct aggatacagt tctcacatca 421 catccgaaca taaacaacca tgggtaagga aaagactcac gtttcgaggc cgcgattaaa 481 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc 541 aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca 601 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac 661 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcttggtt 721 actcaccact gcgatccccg gcaaaacagc attccaggta ttagaagaat atcctgattc 781 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt 841 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat 901 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga 961 acaagtctgg aaagaaatgc acaagctttt gccattctca ccggattcag tcgtcactca 1021 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga 1081 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct 1141 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc 1201 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagtactgac 1261 aataaaaaga ttcttgtttt caagaacttg tcatttgtat agttttttta tattgtagtt 1321 gttctatttt aatcaaatgt tagcgtgatt tatatttttt ttcgcctcga catcatctgc 1381 ccagatgcga agttaagtgc gcagaaagta atatcatgcg tcaatcgtat gtgaatgctg 1441 gtcgctatac tgctgtcgat tcgatactaa cgccgccatc cagtgtcgaa aacgagctct 1501 cgagaaccct taatataact tcgtataatg tatgctatac gaagttatgc tagccctgag 1561 ttcaattcta gcgcaaagga attaccaaga ccattggccg ctagccctgc aggatgcagg 1621 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 1681 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 1741 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 1801 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 1861 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 1921 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 1981 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 2041 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 2101 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 2161 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 2221 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 2281 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 2341 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 2401 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 2461 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 2521 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 2581 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 2641 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 2701 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 2761 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 2821 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 2881 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 2941 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 3001 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 3061 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 3121 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 3181 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 3241 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 3301 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 3361 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 3421 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 3481 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 3541 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca 3601 cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat ttctggaatt 3661 gcctgcaggc ctagggtgtg aaccaatgta tccagcacca cctgtaacca aaacaatttt 3721 cctagggaat tcgcggccgc ttctagag //