BBa_K300003
1
BBa_K300003
Phasin (PhaP) - internal domain
2010-10-02T11:00:00Z
2015-05-08T01:11:51Z
It is identical to <partinfo>BBa_K208001</partinfo>, but it lacks the stop codon in order to support protein fusions.
It has been designed as a head domain:
The Prefix sequence is 5'-GAATTCGCGGCCGCTTCTAG-3' (RFC10 Prefix)
The Suffix sequence is 5'-ACTAGTAGCGGCCGCTGCAG-3' (RFC23 Suffix)
For these reasons, a tail domain or an internal domain (compatible with RFC23) are ready to be assembled downstream to create protein fusions.
'''CONSTRUCTION METHOD:'''
<partinfo>BBa_K208001</partinfo> (provided by iGEM HQ in pSB3K3 in RFC23 standard) was PCR-amplified/mutagenized with primers:
phaPSF: 5'GCTTCTAGAATGATCCTCACCCCGGAACA-3'
phaPSR: 5'-GCTACTAGTGGCAGCCGTCGTCTTCTTTG-3'
in order to delete the stop codon.
The PCR product was ran on a 1% agarose gel, gel-extracted, cut with XbaI-SpeI and ligated with <partinfo>pSB1A3</partinfo> (previously cut with XbaI-SpeI and dephosphorylated).
Positive clones were found through digestion screening/sequencing.
-
false
true
_420_
0
2621
9
It's complicated
true
Ralstonia eutropha genomic DNA.
false
Giacomo Zambianchi, Alessandro Ranieri, Manuel Lupotto, Paolo Magni
BBa_K300077
1
scar
RFC23 scar sequence
2010-10-16T11:00:00Z
2015-05-08T01:11:51Z
RFC23 scar sequence (Silver-Silver mixed site)
false
true
_420_
0
4140
9
Not in stock
false
false
Giacomo Zambianchi, Lorenzo Pasotti, Paolo Magni
BBa_K300080
1
BBa_K300080
Phasin (PhaP) - internal domain - with flexible protein domain linker downstream
2010-10-16T11:00:00Z
2015-05-08T01:11:51Z
This is an internal domain phasin that is ligated to a 10aa flexible protein domain linker (<partinfo>BBa_K105012<partinfo>). Phasin works as reported in <partinfo>BBa_K300003</partinfo>; while the linker has the function to improve, enhance and ease the folding of fusion proteins.
false
true
_420_
0
4140
9
It's complicated
true
false
Giacomo Zambianchi, Sara Bisi, Paolo Magni
component2088063
1
BBa_K300003
component2088064
1
BBa_K300077
component2088065
1
BBa_K105012
annotation2088065
1
BBa_K105012
range2088065
1
583
612
annotation2088064
1
BBa_K300077
range2088064
1
577
582
annotation2088063
1
BBa_K300003
range2088063
1
1
576
BBa_K105012
1
BBa_K105012
10 aa flexible protein domain linker
2008-10-18T11:00:00Z
2015-05-08T01:08:52Z
Oligos with a yeast optimized coding for the peptide GENLYFQSGG have been ordered
<br\n>
The amino acide sequence corresponds to the interdomaine linker of xxx.
''paper''
This is a 10 amino acides long linker peptide which can be used to join protein domains together in a flexible way. So fusion proteins with variable DNA-binding and activation or repression domains might be assembled.
<br\n>
false
true
_253_
0
3313
9
In stock
true
To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction side and the BioBrick. Furthermore, this coding sequence does not include a start codon.<br\n>
For more information about this issus, see:<br\n>
Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." <br\n>
DSpace http://hdl.handle.net/1721.1/32535 (2006).
true
Manuel Gersbacher, Katja Karstens
BBa_K300080_sequence
1
atgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaaccttcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgccactagaggtgaaaatttgtattttcaatctggtggt
BBa_K300003_sequence
1
atgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaaccttcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgcc
BBa_K300077_sequence
1
actaga
BBa_K105012_sequence
1
ggtgaaaatttgtattttcaatctggtggt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z