LOCUS       BBa_K300089             2054 bp    DNA     linear       17-May-2024
DEFINITION  BBa_K300090 construction intermediate
ACCESSION   BBa_K300089
VERSION     BBa_K300089.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=Start
     misc_feature    1..576
                     /label=BBa_K300002
     misc_feature    577..582
                     /label=BBa_K300077
     misc_feature    583..612
                     /label=BBa_K105012
     misc_feature    613..618
                     /label=BBa_K300077
     misc_feature    619..1194
                     /label=BBa_K300003
     misc_feature    1195..1200
                     /label=BBa_K300077
     CDS             1201..1918
                     /label=GFP fusion brick
     misc_feature    1201..1201
                     /label=deletion of TG
     CDS             1201..1918
                     /label=BBa_K125500
     misc_feature    1919..1925
                     /label=BBa_K300076
     stem_loop       1937..1980
                     /label=stem_loop
     misc_feature    1926..2005
                     /label=BBa_B0010
     terminator      1926..2005
                     /label=BBa_B0010
     stem_loop       2021..2040
                     /label=T7 TE
     misc_feature    2047..2047
                     /label=stop
     polyA_site      2041..2054
                     /label=polya
     misc_feature    2014..2054
                     /label=BBa_B0012
     terminator      2014..2054
                     /label=BBa_B0012
     terminator      1926..2054
                     /label=BBa_B0015
     misc_feature    1201..2054
                     /label=BBa_K300005
ORIGIN
        1 atgatcctca ccccggaaca agttgcagca gcgcaaaagg ccaacctcga aacgctgttc
       61 ggcctgacca ccaaggcgtt tgaaggcgtc gaaaagctcg tcgagctgaa ccttcaggtc
      121 gtcaagactt cgttcgcaga aggcgttgac aacgccaaga aggcgctgtc ggccaaggac
      181 gcacaggaac tgctggccat ccaggccgca gccgtgcagc cggttgccga aaagaccctg
      241 gcctacaccc gccacctgta tgaaatcgct tcggaaaccc agagcgagtt caccaaggta
      301 gccgaggctc aactggccga aggctcgaag aacgtgcaag cgctggtcga gaacctcgcc
      361 aagaacgccc cggccggttc ggaatcgacc gtggccatcg tgaagtcggc gatctccgct
      421 gccaacaacg cctacgagtc ggtgcagaag gcgaccaagc aagcggtcga aatcgctgaa
      481 accaacttcc aggctgcggc tacggctgcc accaaggctg cccagcaagc cagcgccacg
      541 gcccgtacgg ccacggcaaa gaagacgacg gctgccacta gaggtgaaaa tttgtatttt
      601 caatctggtg gtactagaat gatcctcacc ccggaacaag ttgcagcagc gcaaaaggcc
      661 aacctcgaaa cgctgttcgg cctgaccacc aaggcgtttg aaggcgtcga aaagctcgtc
      721 gagctgaacc ttcaggtcgt caagacttcg ttcgcagaag gcgttgacaa cgccaagaag
      781 gcgctgtcgg ccaaggacgc acaggaactg ctggccatcc aggccgcagc cgtgcagccg
      841 gttgccgaaa agaccctggc ctacacccgc cacctgtatg aaatcgcttc ggaaacccag
      901 agcgagttca ccaaggtagc cgaggctcaa ctggccgaag gctcgaagaa cgtgcaagcg
      961 ctggtcgaga acctcgccaa gaacgccccg gccggttcgg aatcgaccgt ggccatcgtg
     1021 aagtcggcga tctccgctgc caacaacgcc tacgagtcgg tgcagaaggc gaccaagcaa
     1081 gcggtcgaaa tcgctgaaac caacttccag gctgcggcta cggctgccac caaggctgcc
     1141 cagcaagcca gcgccacggc ccgtacggcc acggcaaaga agacgacggc tgccactaga
     1201 cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
     1261 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
     1321 cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc
     1381 actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat
     1441 gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa
     1501 gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat
     1561 agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg
     1621 gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc
     1681 aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat
     1741 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg
     1801 tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt
     1861 gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa ataataatac
     1921 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     1981 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     2041 tttctgcgtt tata
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