LOCUS BBa_K300092 3690 bp DNA linear 17-May-2024 DEFINITION 3OC6HSL inducible generator for BBa_K300084 ACCESSION BBa_K300092 VERSION BBa_K300092.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..82 /label=prefix misc_feature 254..254 /label=T misc_feature 572..572 /label=A CDS 81..830 /label=luxR misc_feature 81..836 /label=BBa_C0062 misc_feature 837..861 /label=Help:Barcodes CDS 81..836 /label=BBa_C0062 stem_loop 881..924 /label=stem_loop misc_feature 870..949 /label=BBa_B0010 terminator 870..949 /label=BBa_B0010 stem_loop 965..984 /label=T7 TE misc_feature 991..991 /label=stop polyA_site 985..998 /label=polya misc_feature 958..998 /label=BBa_B0012 terminator 958..998 /label=BBa_B0012 terminator 870..998 /label=BBa_B0015 misc_feature 1007..1026 /label=LuxR/HSL promoter 1026..1031 /label=-35 promoter 1048..1053 /label=-10 misc_feature 1059..1059 /label=start misc_feature 1007..1061 /label=BBa_R0062 promoter 1007..1061 /label=BBa_R0062 misc_feature 1..1061 /label=BBa_F2620 misc_feature 1074..1077 /label=conserved RBS 1070..1081 /label=BBa_B0034 misc_feature 1088..1090 /label=Start misc_feature 1088..1663 /label=BBa_K300002 misc_feature 1..1663 /label=BBa_K300082 misc_feature 1664..1669 /label=BBa_K300077 misc_feature 1670..1699 /label=BBa_K105012 misc_feature 1700..1705 /label=BBa_K300077 misc_feature 1706..2281 /label=BBa_K300003 misc_feature 2282..2287 /label=BBa_K300077 misc_feature 2288..2317 /label=BBa_K105012 misc_feature 2318..2323 /label=BBa_K300077 misc_feature 2324..2830 /label=BBa_K300004 misc_feature 2831..2836 /label=BBa_K300077 CDS 2837..3554 /label=GFP fusion brick misc_feature 2837..2837 /label=deletion of TG CDS 2837..3554 /label=BBa_K125500 misc_feature 3555..3561 /label=BBa_K300076 stem_loop 3573..3616 /label=stem_loop misc_feature 3562..3641 /label=BBa_B0010 terminator 3562..3641 /label=BBa_B0010 stem_loop 3657..3676 /label=T7 TE misc_feature 3683..3683 /label=stop polyA_site 3677..3690 /label=polya misc_feature 3650..3690 /label=BBa_B0012 terminator 3650..3690 /label=BBa_B0012 terminator 3562..3690 /label=BBa_B0015 misc_feature 2837..3690 /label=BBa_K300005 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt 1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa 1081 atactagatg atcctcaccc cggaacaagt tgcagcagcg caaaaggcca acctcgaaac 1141 gctgttcggc ctgaccacca aggcgtttga aggcgtcgaa aagctcgtcg agctgaacct 1201 tcaggtcgtc aagacttcgt tcgcagaagg cgttgacaac gccaagaagg cgctgtcggc 1261 caaggacgca caggaactgc tggccatcca ggccgcagcc gtgcagccgg ttgccgaaaa 1321 gaccctggcc tacacccgcc acctgtatga aatcgcttcg gaaacccaga gcgagttcac 1381 caaggtagcc gaggctcaac tggccgaagg ctcgaagaac gtgcaagcgc tggtcgagaa 1441 cctcgccaag aacgccccgg ccggttcgga atcgaccgtg gccatcgtga agtcggcgat 1501 ctccgctgcc aacaacgcct acgagtcggt gcagaaggcg accaagcaag cggtcgaaat 1561 cgctgaaacc aacttccagg ctgcggctac ggctgccacc aaggctgccc agcaagccag 1621 cgccacggcc cgtacggcca cggcaaagaa gacgacggct gccactagag gtgaaaattt 1681 gtattttcaa tctggtggta ctagaatgat cctcaccccg gaacaagttg cagcagcgca 1741 aaaggccaac ctcgaaacgc tgttcggcct gaccaccaag gcgtttgaag gcgtcgaaaa 1801 gctcgtcgag ctgaaccttc aggtcgtcaa gacttcgttc gcagaaggcg ttgacaacgc 1861 caagaaggcg ctgtcggcca aggacgcaca ggaactgctg gccatccagg ccgcagccgt 1921 gcagccggtt gccgaaaaga ccctggccta cacccgccac ctgtatgaaa tcgcttcgga 1981 aacccagagc gagttcacca aggtagccga ggctcaactg gccgaaggct cgaagaacgt 2041 gcaagcgctg gtcgagaacc tcgccaagaa cgccccggcc ggttcggaat cgaccgtggc 2101 catcgtgaag tcggcgatct ccgctgccaa caacgcctac gagtcggtgc agaaggcgac 2161 caagcaagcg gtcgaaatcg ctgaaaccaa cttccaggct gcggctacgg ctgccaccaa 2221 ggctgcccag caagccagcg ccacggcccg tacggccacg gcaaagaaga cgacggctgc 2281 cactagaggt gaaaatttgt attttcaatc tggtggtact agagcactgg ctgaaggaac 2341 ccgtattttt gatccggtga caggcacaac tcatcgtatc gaggatgtgg ttggtgggcg 2401 taaaccgatt catgtggtgg cagctgctaa agatggtaca ctgcatgcac gtccggtagt 2461 aagctggttc gatcagggta cacgtgatgt gattggactg cgtattgccg gtggcgctat 2521 tctgtgggct actcctgacc ataaagtcct gacggaatat ggatggcgtg ctgctggtga 2581 actgcgcaaa ggtgaccgtg ttgctcaacc tcgtcgtttc gatggttttg gcgattctgc 2641 cccaattcct gctcgtgttc aggctctggc tgatgccctg gatgacaaat ttctgcacga 2701 tatgctggcc gaagaactgc gttattcagt gatccgtgaa gtcctgccta cacgtcgtgc 2761 tcgtacattt ggtctggaag tggaagagct gcatacactg gttgctgagg gtgttgtagt 2821 acacaattgc actagacgta aaggagaaga acttttcact ggagttgtcc caattcttgt 2881 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga 2941 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc 3001 atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga 3061 tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag 3121 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg 3181 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat 3241 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa 3301 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt 3361 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc 3421 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga 3481 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact 3541 atacaaataa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 3601 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 3661 caccttcggg tgggcctttc tgcgtttata //