LOCUS       BBa_K300982             2151 bp    DNA     linear       09-May-2024
DEFINITION  BBa_K300000 construction intermediate
ACCESSION   BBa_K300982
VERSION     BBa_K300982.1  
FEATURES             Location/Qualifiers
     misc_feature    1..7
                     /label=BBa_K300994
     misc_feature    8..413
                     /label=BBa_K300998
     misc_feature    414..421
                     /label=BBa_G0001
     misc_feature    422..462
                     /label=B0012
     misc_feature    471..550
                     /label=B0010
     terminator      422..550
                     /label=BBa_B0025
     misc_feature    551..558
                     /label=BBa_G0001
     CDS             559..1221
                     /label=cmR(rev)
     misc_feature    559..1327
                     /label=BBa_K300999
     misc_feature    1328..1334
                     /label=BBa_K300995
     misc_feature    1335..1370
                     /label=BBa_J72001
     misc_feature    1371..1376
                     /label=NheI site
     misc_feature    1371..1376
                     /label=BBa_B0045
     misc_feature    1377..1786
                     /label=BBa_K300991
     misc_feature    1787..1792
                     /label=NheI site
     misc_feature    1787..1792
                     /label=BBa_B0045
     stem_loop       1801..1835
                     /label=stem_loop
     terminator      1793..1864
                     /label=BBa_B0053
     primer_bind     1865..1884
                     /label=VF2
     primer          1865..1884
                     /label=BBa_G00100
     stem_loop       1920..1941
                     /label=stem loop from bacteriophage T3
     misc_feature    1912..1950
                     /label=identical to bacteriophage T3
     terminator      1885..1962
                     /label=BBa_B0055
     misc_feature    1963..1968
                     /label=BBa_M31983
     RBS             1969..1979
                     /label=RBS-4\Weaker
     RBS             1975..1978
                     /label=RBS
     misc_feature    1969..1979
                     /label=BBa_B0033
     RBS             1969..1979
                     /label=BBa_B0033
     misc_feature    1980..1985
                     /label=BBa_K300981
     stem_loop       1996..2027
                     /label=stem loop
     misc_feature    1989..2028
                     /label=identical to E. coli MG1655 between tonB and yciA
     terminator      1986..2054
                     /label=BBa_B0054
     primer_bind     2055..2074
                     /label=VR
     misc_feature    2055..2074
                     /label=BBa_G00102
     stem_loop       2088..2107
                     /label=stem_loop
     terminator      2075..2115
                     /label=BBa_B0062
     misc_feature    2116..2151
                     /label=BBa_J72001
ORIGIN
        1 cctagtagca gttcaacctg ttgatagtac gtactaagct ctcatgtttc acgtactaag
       61 ctctcatgtt taacgtacta agctctcatg tttaacgaac taaaccctca tggctaacgt
      121 actaagctct catggctaac gtactaagct ctcatgtttc acgtactaag ctctcatgtt
      181 tgaacaataa aattaatata aatcagcaac ttaaatagcc tctaaggttt taagttttat
      241 aagaaaaaaa agaatatata aggcttttaa agcttttaag gtttaacggt tgtggacaac
      301 aagccaggga tgtaacgcac tgagaagccc ttagagcctc tcaaagcaat tttgagtgac
      361 acaggaacac ttaacggctg acatgggaat tagccatggg cccgtgcgaa tcactctagt
      421 atataaacgc agaaaggccc acccgaaggt gagccagtgt gactctagta gagagcgttc
      481 accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt
      541 tgatgcctgg ctctagtatt attacgcccc gccctgccac tcatcacagt actgttgtaa
      601 ttcattaagc atgcggccga catggaagcc atcacaaacg gcatgatgaa cctggatcgc
      661 cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatcgtga aaacgggggc
      721 gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt
      781 ggctgagaca aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc
      841 gtaacacgcc acatcttgcg aatatatgtg gagaaactgc cggaaatcgt cgtggtattc
      901 actccagagg gacgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac
      961 actatcccag atcaccagct caccgtcttt catggccata cgaaactccg ggtgagcgtt
     1021 catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac
     1081 ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac
     1141 tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata
     1201 tcccgtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa
     1261 aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccc
     1321 gatcaacctc tagggcgaag ttcctatact ttctagagaa taggaacttc gctagccatg
     1381 ggcccgtgcg aatcagaaat aatctcgata tcatctcttt tcttgccgag cgccttcctg
     1441 agctttgtgt cggtgatcat tgaatgggta cacatttttg tttttgggta cacaaaagtg
     1501 tacacaaagt tgcccactca aagctacacg caatgtaaca ctagttcgca gagtgttatg
     1561 gtttacatcc ttgaaagcct gctggataag ggtttagcgt aacagaacgt ttttacgcgg
     1621 aattgttcgt aatatgccaa atgacaattt aagaaagtgt tctaatttat tagaaatttg
     1681 cacttaaatc aaaaagttac ggacaattca accaccaatc aataaattaa agggcacatt
     1741 aaagtacaca atatttgtgc ccttctctgt ttctttcccg ttatcagcta gctccggcaa
     1801 aaaaacgggc aaggtgtcac caccctgccc tttttcttta aaaccgaaaa gattacttcg
     1861 cgtttgccac ctgacgtcta agaaaaggaa tattcagcaa tttgcccgtg ccgaagaaag
     1921 gcccacccgt gaaggtgagc cagtgagttg attgctacgt aagaattctc acacaggacc
     1981 tgcagattag cagaaagtca aaagcctccg accggaggct tttgactaaa acttcccttg
     2041 gggttatcat tggggctcac tcaaaggcgg taatcagata aaaaaaatcc ttagctttcg
     2101 ctaaggatga tttctgcgaa gttcctatac tttctagaga ataggaactt c
//