BBa_K300994 1 scar SpeI/AvrII mixed site (when SpeI comes from RFC10 Prefix) - rev 2010-10-01T11:00:00Z 2015-05-08T01:11:52Z - Reverse complement of <partinfo>BBa_K300996</partinfo>. false false _420_ 0 2621 9 Not in stock false See <partinfo>BBa_K300996</partinfo> false Lorenzo Pasotti, Matteo Meroso, Nicolo' Politi, Paolo Magni BBa_B0033 1 BBa_B0033 RBS.4 (weaker) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weaker RBS based on Ron Weiss thesis. Strengths relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-3&quot; in figure 4-14 of thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7028 1 BBa_B0033 range7028 1 1 11 annotation1713 1 RBS-4\Weaker range1713 1 1 11 annotation1714 1 RBS range1714 1 7 10 BBa_B0025 1 BBa_B0025 double terminator (B0015), reversed 2003-12-02T12:00:00Z 2015-08-31T04:07:20Z -- No description -- false true _1_ 0 24 7 In stock false true Caitlin Conboy annotation369703 1 B0010 range369703 1 50 129 annotation369702 1 B0012 range369702 1 1 41 BBa_G00100 1 VF2 Forward primer for sequencing/amplifying BioBrick parts (VF2) 2004-05-24T11:00:00Z 2015-08-31T04:07:28Z Originally named VF2, primer binds upstream of the standard BBa_MCS on biobrick vectors. false false _11_1_ 0 60 7 Not in stock false false Tom Knight annotation1934505 1 VF2 range1934505 1 1 20 BBa_B0053 1 BBa_B0053 Terminator (His) 2004-01-29T12:00:00Z 2015-08-31T04:07:20Z Terminator from the E.coli His operon false true _1_ 0 24 7 Not in stock false false Caitlin Conboy annotation318602 1 stem_loop range318602 1 9 43 BBa_J72001 1 FRT {FRT} recombination site for flp recombinase in BBb 2008-07-14T11:00:00Z 2015-05-08T01:08:26Z Later Later false false _171_ 0 483 171 Not in stock false N/A false John Anderson BBa_B0055 1 BBa_B0055 -- No description -- 2005-11-16T12:00:00Z 2015-08-31T04:07:20Z Terminator to be placed upstream of multiple cloning site in new pSB series plasmids. false false _41_6_ 0 126 44 Not in stock false false Reshma Shetty annotation1783311 1 identical to bacteriophage T3 range1783311 1 28 66 annotation1783301 1 stem loop from bacteriophage T3 range1783301 1 36 57 BBa_K300981 1 PstI PstI restriction enzyme site 2010-10-08T11:00:00Z 2015-05-08T01:11:51Z none EcoRI restriction enzyme site false false _420_ 0 2621 9 Not in stock false none false Lorenzo Pasotti, Paolo Magni BBa_B0054 1 BBa_B0054 Transcriptional terminator 2005-11-16T12:00:00Z 2015-08-31T04:07:20Z Terminator to be placed downstream of multiple cloning site in new pSB series plasmids. Similar to BBa_B0051. false false _41_6_ 0 126 44 Not in stock false false Reshma Shetty annotation1783342 1 identical to E. coli MG1655 between tonB and yciA range1783342 1 4 43 annotation1747359 1 stem loop range1747359 1 11 42 BBa_G00102 1 VR Reverse BioBrick primer annealing site (VR binding site) 2006-02-03T12:00:00Z 2015-08-31T04:07:28Z This is the VR sequence to be used in construction of plasmids which include the VR primer site. It is the reverse complement of BBa_G00101. false false _41_6_ 0 126 45 Not in stock false false Reshma Shetty annotation1961229 1 VR range1961229 1 1 20 BBa_K300991 1 phi80 attP phi80 phage attP integration site 2010-10-01T11:00:00Z 2015-05-08T01:11:52Z - This part is equal to <partinfo>BBa_J72004</partinfo>, but it lacks the NheI restriction site (GCTAGC) at the 3' end. false false _420_ 0 2621 9 Not in stock true See <partinfo>BBa_J72004</partinfo> for more information. false Lorenzo Pasotti, Manuel Lupotto, Paolo Magni BBa_K300982 1 BBa_K300982 BBa_K300000 construction intermediate 2010-10-08T11:00:00Z 2015-05-08T01:11:52Z <partinfo>BBa_K300983</partinfo> - Mr Gene synthesis service; <partinfo>BBa_K300008</partinfo> - UNIPV-Pavia iGEM 2010 Team. This part was used to construct <partinfo>BBa_K300000</partinfo> BioBrick integrative base vector. false false _420_ 0 2621 9 Not in stock false See <partinfo>BBa_K300000</partinfo> for details about the design of this part. false Lorenzo Pasotti, Matteo Meroso, Nicolo' Politi, Paolo Magni component2084559 1 BBa_B0055 component2084571 1 BBa_B0062 component2084546 1 BBa_K300995 component2084543 1 BBa_G0001 component2084554 1 BBa_B0053 component2084560 1 BBa_M31983 component2084562 1 BBa_B0033 component2084545 1 BBa_K300999 component2084537 1 BBa_K300994 component2084567 1 BBa_B0054 component2084542 1 BBa_B0025 component2084538 1 BBa_K300998 component2084552 1 BBa_B0045 component2084556 1 BBa_G00100 component2084569 1 BBa_G00102 component2084549 1 BBa_B0045 component2084550 1 BBa_K300991 component2084547 1 BBa_J72001 component2084564 1 BBa_K300981 component2084572 1 BBa_J72001 component2084539 1 BBa_G0001 annotation2084567 1 BBa_B0054 range2084567 1 1986 2054 annotation2084569 1 BBa_G00102 range2084569 1 2055 2074 annotation2084562 1 BBa_B0033 range2084562 1 1969 1979 annotation2084556 1 BBa_G00100 range2084556 1 1865 1884 annotation2084539 1 BBa_G0001 range2084539 1 414 421 annotation2084546 1 BBa_K300995 range2084546 1 1328 1334 annotation2084560 1 BBa_M31983 range2084560 1 1963 1968 annotation2084571 1 BBa_B0062 range2084571 1 2075 2115 annotation2084547 1 BBa_J72001 range2084547 1 1335 1370 annotation2084559 1 BBa_B0055 range2084559 1 1885 1962 annotation2084549 1 BBa_B0045 range2084549 1 1371 1376 annotation2084545 1 BBa_K300999 range2084545 1 559 1327 annotation2084537 1 BBa_K300994 range2084537 1 1 7 annotation2084542 1 BBa_B0025 range2084542 1 422 550 annotation2084554 1 BBa_B0053 range2084554 1 1793 1864 annotation2084572 1 BBa_J72001 range2084572 1 2116 2151 annotation2084552 1 BBa_B0045 range2084552 1 1787 1792 annotation2084564 1 BBa_K300981 range2084564 1 1980 1985 annotation2084550 1 BBa_K300991 range2084550 1 1377 1786 annotation2084538 1 BBa_K300998 range2084538 1 8 413 annotation2084543 1 BBa_G0001 range2084543 1 551 558 BBa_M31983 1 EcoRI EcoRI restriction enzyme site 2007-02-28T12:00:00Z 2015-05-08T01:14:01Z none EcoRI restriction enzyme site false true _102_ 0 1373 102 Not in stock false none false Tiffany Guo BBa_B0045 1 NheI NheI restriction enzyme site (XbaI, SpeI compatible overhang) 2006-03-13T12:00:00Z 2015-08-31T04:07:20Z NheI recognition site. Digestion with NheI generates a compatible cohesive end to XbaI and SpeI. false true _41_6_ 0 126 45 Not in stock false false Reshma Shetty annotation1822724 1 NheI site range1822724 1 1 6 BBa_K300999 1 CmR chloramphenicol resistance cassette (rev) 2010-10-01T11:00:00Z 2015-05-08T01:11:52Z <partinfo>BBa_P1004</partinfo> Reverse complement of <partinfo>BBa_P1004</partinfo> (chloramphenicol resistance cassette without transcriptional terminator). false false _420_ 0 2621 9 Not in stock false see <partinfo>BBa_P1004</partinfo> for detailed information. false Lorenzo Pasotti, Manuel Lupotto, Paolo Magni annotation2081291 1 cmR(rev) range2081291 1 1 663 BBa_G0001 1 scar XbaI/SpeI mixed site, reverse 2007-02-26T12:00:00Z 2015-08-31T04:07:27Z XbaI and SpeI XbaI/SpeI mixed site, reverse false false _41_ 0 126 70 Not in stock false None. false Reshma Shetty BBa_K300998 1 R6K ori [R6K] Origin of replication (rev) 2010-10-01T11:00:00Z 2015-05-08T01:11:52Z See <partinfo>BBa_J61001</partinfo> for detailed information. Reverse complement of <partinfo>BBa_J61001</partinfo> (R6K conditional replication origin). false false _420_ 0 2621 9 Not in stock false <partinfo>BBa_J61001</partinfo> false Lorenzo Pasotti, Manuel Lupotto, Paolo Magni BBa_B0062 1 BBa_B0062 Terminator (Reverse B0052) 2004-01-29T12:00:00Z 2015-08-31T04:07:21Z Reverse of B0052, E.coli transcriptional terminator from the ribosomal RNA rrnC operon. false false _1_ 0 24 7 Not in stock false false Caitlin Conboy annotation318637 1 stem_loop range318637 1 14 33 BBa_K300995 1 scar AvrII/XbaI mixed site (when XbaI comes from RFC10 Prefix) - rev 2010-10-01T11:00:00Z 2015-05-08T01:11:52Z - Reverse complement of <partinfo>BBa_K300997</partinfo> false false _420_ 0 2621 9 Not in stock false See <partinfo>BBa_K300997</partinfo> false Lorenzo Pasotti, Matteo Meroso, Nicolo' Politi, Paolo Magni BBa_B0033_sequence 1 tcacacaggac BBa_K300981_sequence 1 ctgcag BBa_B0045_sequence 1 gctagc BBa_K300995_sequence 1 ctctagg BBa_J72001_sequence 1 gcgaagttcctatactttctagagaataggaacttc BBa_K300998_sequence 1 gcagttcaacctgttgatagtacgtactaagctctcatgtttcacgtactaagctctcatgtttaacgtactaagctctcatgtttaacgaactaaaccctcatggctaacgtactaagctctcatggctaacgtactaagctctcatgtttcacgtactaagctctcatgtttgaacaataaaattaatataaatcagcaacttaaatagcctctaaggttttaagttttataagaaaaaaaagaatatataaggcttttaaagcttttaaggtttaacggttgtggacaacaagccagggatgtaacgcactgagaagcccttagagcctctcaaagcaattttgagtgacacaggaacacttaacggctgacatgggaattagccatgggcccgtgcgaatca BBa_B0062_sequence 1 cagataaaaaaaatccttagctttcgctaaggatgatttct BBa_M31983_sequence 1 gaattc BBa_B0025_sequence 1 tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctgg BBa_B0055_sequence 1 aaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaa BBa_B0054_sequence 1 attagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggg BBa_K300991_sequence 1 catgggcccgtgcgaatcagaaataatctcgatatcatctcttttcttgccgagcgccttcctgagctttgtgtcggtgatcattgaatgggtacacatttttgtttttgggtacacaaaagtgtacacaaagttgcccactcaaagctacacgcaatgtaacactagttcgcagagtgttatggtttacatccttgaaagcctgctggataagggtttagcgtaacagaacgtttttacgcggaattgttcgtaatatgccaaatgacaatttaagaaagtgttctaatttattagaaatttgcacttaaatcaaaaagttacggacaattcaaccaccaatcaataaattaaagggcacattaaagtacacaatatttgtgcccttctctgtttctttcccgttatca BBa_K300999_sequence 1 ttattacgccccgccctgccactcatcacagtactgttgtaattcattaagcatgcggccgacatggaagccatcacaaacggcatgatgaacctggatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatcgtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacaaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtggagaaactgccggaaatcgtcgtggtattcactccagagggacgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccagatcaccagctcaccgtctttcatggccatacgaaactccgggtgagcgttcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatcccgtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaac BBa_B0053_sequence 1 tccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtt BBa_G00100_sequence 1 tgccacctgacgtctaagaa BBa_G0001_sequence 1 ctctagta BBa_K300982_sequence 1 cctagtagcagttcaacctgttgatagtacgtactaagctctcatgtttcacgtactaagctctcatgtttaacgtactaagctctcatgtttaacgaactaaaccctcatggctaacgtactaagctctcatggctaacgtactaagctctcatgtttcacgtactaagctctcatgtttgaacaataaaattaatataaatcagcaacttaaatagcctctaaggttttaagttttataagaaaaaaaagaatatataaggcttttaaagcttttaaggtttaacggttgtggacaacaagccagggatgtaacgcactgagaagcccttagagcctctcaaagcaattttgagtgacacaggaacacttaacggctgacatgggaattagccatgggcccgtgcgaatcactctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtattattacgccccgccctgccactcatcacagtactgttgtaattcattaagcatgcggccgacatggaagccatcacaaacggcatgatgaacctggatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatcgtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacaaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtggagaaactgccggaaatcgtcgtggtattcactccagagggacgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccagatcaccagctcaccgtctttcatggccatacgaaactccgggtgagcgttcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatcccgtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaacctctagggcgaagttcctatactttctagagaataggaacttcgctagccatgggcccgtgcgaatcagaaataatctcgatatcatctcttttcttgccgagcgccttcctgagctttgtgtcggtgatcattgaatgggtacacatttttgtttttgggtacacaaaagtgtacacaaagttgcccactcaaagctacacgcaatgtaacactagttcgcagagtgttatggtttacatccttgaaagcctgctggataagggtttagcgtaacagaacgtttttacgcggaattgttcgtaatatgccaaatgacaatttaagaaagtgttctaatttattagaaatttgcacttaaatcaaaaagttacggacaattcaaccaccaatcaataaattaaagggcacattaaagtacacaatatttgtgcccttctctgtttctttcccgttatcagctagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaagaattctcacacaggacctgcagattagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagataaaaaaaatccttagctttcgctaaggatgatttctgcgaagttcctatactttctagagaataggaacttc BBa_K300994_sequence 1 cctagta BBa_G00102_sequence 1 gctcactcaaaggcggtaat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z