LOCUS       BBa_K300983              829 bp    DNA     linear       09-May-2024
DEFINITION  BBa_K300000 construction intermediate (provided by Mr Gene)
ACCESSION   BBa_K300983
VERSION     BBa_K300983.1  
FEATURES             Location/Qualifiers
     misc_feature    1..6
                     /label=AvrII site
     misc_feature    1..6
                     /label=BBa_B0048
     misc_feature    7..42
                     /label=BBa_J72001
     misc_feature    43..48
                     /label=NheI site
     misc_feature    43..48
                     /label=BBa_B0045
     misc_feature    49..458
                     /label=BBa_K300991
     misc_feature    459..464
                     /label=NheI site
     misc_feature    459..464
                     /label=BBa_B0045
     stem_loop       473..507
                     /label=stem_loop
     terminator      465..536
                     /label=BBa_B0053
     primer_bind     537..556
                     /label=VF2
     primer          537..556
                     /label=BBa_G00100
     stem_loop       592..613
                     /label=stem loop from bacteriophage T3
     misc_feature    584..622
                     /label=identical to bacteriophage T3
     terminator      557..634
                     /label=BBa_B0055
     misc_feature    635..640
                     /label=BBa_M31983
     RBS             641..651
                     /label=RBS-4\Weaker
     RBS             647..650
                     /label=RBS
     misc_feature    641..651
                     /label=BBa_B0033
     RBS             641..651
                     /label=BBa_B0033
     misc_feature    652..657
                     /label=BBa_K300981
     stem_loop       668..699
                     /label=stem loop
     misc_feature    661..700
                     /label=identical to E. coli MG1655 between tonB and yciA
     terminator      658..726
                     /label=BBa_B0054
     primer_bind     727..746
                     /label=VR
     misc_feature    727..746
                     /label=BBa_G00102
     stem_loop       760..779
                     /label=stem_loop
     terminator      747..787
                     /label=BBa_B0062
     misc_feature    788..823
                     /label=BBa_J72001
     misc_feature    824..829
                     /label=AvrII site
     misc_feature    824..829
                     /label=BBa_B0048
ORIGIN
        1 cctagggcga agttcctata ctttctagag aataggaact tcgctagcca tgggcccgtg
       61 cgaatcagaa ataatctcga tatcatctct tttcttgccg agcgccttcc tgagctttgt
      121 gtcggtgatc attgaatggg tacacatttt tgtttttggg tacacaaaag tgtacacaaa
      181 gttgcccact caaagctaca cgcaatgtaa cactagttcg cagagtgtta tggtttacat
      241 ccttgaaagc ctgctggata agggtttagc gtaacagaac gtttttacgc ggaattgttc
      301 gtaatatgcc aaatgacaat ttaagaaagt gttctaattt attagaaatt tgcacttaaa
      361 tcaaaaagtt acggacaatt caaccaccaa tcaataaatt aaagggcaca ttaaagtaca
      421 caatatttgt gcccttctct gtttctttcc cgttatcagc tagctccggc aaaaaaacgg
      481 gcaaggtgtc accaccctgc cctttttctt taaaaccgaa aagattactt cgcgtttgcc
      541 acctgacgtc taagaaaagg aatattcagc aatttgcccg tgccgaagaa aggcccaccc
      601 gtgaaggtga gccagtgagt tgattgctac gtaagaattc tcacacagga cctgcagatt
      661 agcagaaagt caaaagcctc cgaccggagg cttttgacta aaacttccct tggggttatc
      721 attggggctc actcaaaggc ggtaatcaga taaaaaaaat ccttagcttt cgctaaggat
      781 gatttctgcg aagttcctat actttctaga gaataggaac ttccctagg
//