BBa_K300983
1
BBa_K300983
BBa_K300000 construction intermediate (provided by Mr Gene)
2010-10-08T11:00:00Z
2015-05-08T01:11:52Z
Mr Gene synthesis service (www.mrgene.com).
This part has been provided by Mr Gene synthesis service and it has been used to construct <partinfo>BBa_K300982</partinfo> plasmid. On the other hand, <partinfo>BBa_K300982</partinfo> was used to construct <partinfo>BBa_K300000</partinfo> BioBrick integrative base vector.
false
false
_420_
0
2621
9
Not in stock
false
See <partinfo>BBa_K300000</partinfo> for details about the design of this part.
false
Lorenzo Pasotti, Matteo Meroso, Nicolo' Politi, Paolo Magni
component2084440
1
BBa_K300991
component2084454
1
BBa_K300981
component2084461
1
BBa_B0062
component2084450
1
BBa_M31983
component2084457
1
BBa_B0054
component2084464
1
BBa_B0048
component2084436
1
BBa_B0048
component2084462
1
BBa_J72001
component2084459
1
BBa_G00102
component2084439
1
BBa_B0045
component2084452
1
BBa_B0033
component2084444
1
BBa_B0053
component2084437
1
BBa_J72001
component2084442
1
BBa_B0045
component2084449
1
BBa_B0055
component2084446
1
BBa_G00100
annotation2084440
1
BBa_K300991
range2084440
1
49
458
annotation2084457
1
BBa_B0054
range2084457
1
658
726
annotation2084464
1
BBa_B0048
range2084464
1
824
829
annotation2084444
1
BBa_B0053
range2084444
1
465
536
annotation2084442
1
BBa_B0045
range2084442
1
459
464
annotation2084452
1
BBa_B0033
range2084452
1
641
651
annotation2084439
1
BBa_B0045
range2084439
1
43
48
annotation2084450
1
BBa_M31983
range2084450
1
635
640
annotation2084459
1
BBa_G00102
range2084459
1
727
746
annotation2084446
1
BBa_G00100
range2084446
1
537
556
annotation2084449
1
BBa_B0055
range2084449
1
557
634
annotation2084461
1
BBa_B0062
range2084461
1
747
787
annotation2084462
1
BBa_J72001
range2084462
1
788
823
annotation2084436
1
BBa_B0048
range2084436
1
1
6
annotation2084437
1
BBa_J72001
range2084437
1
7
42
annotation2084454
1
BBa_K300981
range2084454
1
652
657
BBa_B0055
1
BBa_B0055
-- No description --
2005-11-16T12:00:00Z
2015-08-31T04:07:20Z
Terminator to be placed upstream of multiple cloning site in new pSB series plasmids.
false
false
_41_6_
0
126
44
Not in stock
false
false
Reshma Shetty
annotation1783311
1
identical to bacteriophage T3
range1783311
1
28
66
annotation1783301
1
stem loop from bacteriophage T3
range1783301
1
36
57
BBa_B0062
1
BBa_B0062
Terminator (Reverse B0052)
2004-01-29T12:00:00Z
2015-08-31T04:07:21Z
Reverse of B0052, E.coli transcriptional terminator from the ribosomal RNA rrnC operon.
false
false
_1_
0
24
7
Not in stock
false
false
Caitlin Conboy
annotation318637
1
stem_loop
range318637
1
14
33
BBa_B0048
1
AvrII
AvrII restriction enzyme site (XbaI, SpeI compatible overhang)
2006-03-13T12:00:00Z
2015-08-31T04:07:20Z
AvrII recognition site. Digestion with AvrII generates a compatible cohesive end to XbaI and SpeI.
false
true
_41_6_
0
126
45
Not in stock
false
false
Reshma Shetty
annotation1822744
1
AvrII site
range1822744
1
1
6
BBa_G00100
1
VF2
Forward primer for sequencing/amplifying BioBrick parts (VF2)
2004-05-24T11:00:00Z
2015-08-31T04:07:28Z
Originally named VF2, primer binds upstream of the standard BBa_MCS on biobrick vectors.
false
false
_11_1_
0
60
7
Not in stock
false
false
Tom Knight
annotation1934505
1
VF2
range1934505
1
1
20
BBa_J72001
1
FRT
{FRT} recombination site for flp recombinase in BBb
2008-07-14T11:00:00Z
2015-05-08T01:08:26Z
Later
Later
false
false
_171_
0
483
171
Not in stock
false
N/A
false
John Anderson
BBa_G00102
1
VR
Reverse BioBrick primer annealing site (VR binding site)
2006-02-03T12:00:00Z
2015-08-31T04:07:28Z
This is the VR sequence to be used in construction of plasmids which include the VR primer site. It is the reverse complement of BBa_G00101.
false
false
_41_6_
0
126
45
Not in stock
false
false
Reshma Shetty
annotation1961229
1
VR
range1961229
1
1
20
BBa_B0045
1
NheI
NheI restriction enzyme site (XbaI, SpeI compatible overhang)
2006-03-13T12:00:00Z
2015-08-31T04:07:20Z
NheI recognition site. Digestion with NheI generates a compatible cohesive end to XbaI and SpeI.
false
true
_41_6_
0
126
45
Not in stock
false
false
Reshma Shetty
annotation1822724
1
NheI site
range1822724
1
1
6
BBa_K300981
1
PstI
PstI restriction enzyme site
2010-10-08T11:00:00Z
2015-05-08T01:11:51Z
none
EcoRI restriction enzyme site
false
false
_420_
0
2621
9
Not in stock
false
none
false
Lorenzo Pasotti, Paolo Magni
BBa_K300991
1
phi80 attP
phi80 phage attP integration site
2010-10-01T11:00:00Z
2015-05-08T01:11:52Z
-
This part is equal to <partinfo>BBa_J72004</partinfo>, but it lacks the NheI restriction site (GCTAGC) at the 3' end.
false
false
_420_
0
2621
9
Not in stock
true
See <partinfo>BBa_J72004</partinfo> for more information.
false
Lorenzo Pasotti, Manuel Lupotto, Paolo Magni
BBa_B0054
1
BBa_B0054
Transcriptional terminator
2005-11-16T12:00:00Z
2015-08-31T04:07:20Z
Terminator to be placed downstream of multiple cloning site in new pSB series plasmids. Similar to BBa_B0051.
false
false
_41_6_
0
126
44
Not in stock
false
false
Reshma Shetty
annotation1747359
1
stem loop
range1747359
1
11
42
annotation1783342
1
identical to E. coli MG1655 between tonB and yciA
range1783342
1
4
43
BBa_M31983
1
EcoRI
EcoRI restriction enzyme site
2007-02-28T12:00:00Z
2015-05-08T01:14:01Z
none
EcoRI restriction enzyme site
false
true
_102_
0
1373
102
Not in stock
false
none
false
Tiffany Guo
BBa_B0033
1
BBa_B0033
RBS.4 (weaker) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weaker RBS based on Ron Weiss thesis. Strengths relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-3" in figure 4-14 of thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1713
1
RBS-4\Weaker
range1713
1
1
11
annotation7028
1
BBa_B0033
range7028
1
1
11
annotation1714
1
RBS
range1714
1
7
10
BBa_B0053
1
BBa_B0053
Terminator (His)
2004-01-29T12:00:00Z
2015-08-31T04:07:20Z
Terminator from the E.coli His operon
false
true
_1_
0
24
7
Not in stock
false
false
Caitlin Conboy
annotation318602
1
stem_loop
range318602
1
9
43
BBa_B0033_sequence
1
tcacacaggac
BBa_K300981_sequence
1
ctgcag
BBa_B0045_sequence
1
gctagc
BBa_J72001_sequence
1
gcgaagttcctatactttctagagaataggaacttc
BBa_B0062_sequence
1
cagataaaaaaaatccttagctttcgctaaggatgatttct
BBa_M31983_sequence
1
gaattc
BBa_B0055_sequence
1
aaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaa
BBa_B0048_sequence
1
cctagg
BBa_B0054_sequence
1
attagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggg
BBa_K300991_sequence
1
catgggcccgtgcgaatcagaaataatctcgatatcatctcttttcttgccgagcgccttcctgagctttgtgtcggtgatcattgaatgggtacacatttttgtttttgggtacacaaaagtgtacacaaagttgcccactcaaagctacacgcaatgtaacactagttcgcagagtgttatggtttacatccttgaaagcctgctggataagggtttagcgtaacagaacgtttttacgcggaattgttcgtaatatgccaaatgacaatttaagaaagtgttctaatttattagaaatttgcacttaaatcaaaaagttacggacaattcaaccaccaatcaataaattaaagggcacattaaagtacacaatatttgtgcccttctctgtttctttcccgttatca
BBa_B0053_sequence
1
tccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtt
BBa_K300983_sequence
1
cctagggcgaagttcctatactttctagagaataggaacttcgctagccatgggcccgtgcgaatcagaaataatctcgatatcatctcttttcttgccgagcgccttcctgagctttgtgtcggtgatcattgaatgggtacacatttttgtttttgggtacacaaaagtgtacacaaagttgcccactcaaagctacacgcaatgtaacactagttcgcagagtgttatggtttacatccttgaaagcctgctggataagggtttagcgtaacagaacgtttttacgcggaattgttcgtaatatgccaaatgacaatttaagaaagtgttctaatttattagaaatttgcacttaaatcaaaaagttacggacaattcaaccaccaatcaataaattaaagggcacattaaagtacacaatatttgtgcccttctctgtttctttcccgttatcagctagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaagaattctcacacaggacctgcagattagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagataaaaaaaatccttagctttcgctaaggatgatttctgcgaagttcctatactttctagagaataggaacttccctagg
BBa_G00100_sequence
1
tgccacctgacgtctaagaa
BBa_G00102_sequence
1
gctcactcaaaggcggtaat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z