LOCUS       BBa_K301006             3082 bp    DNA     linear       01-Jun-2024
DEFINITION  Low Oxygen Level Self-induction Genetic Circuit. NarK promoter
ACCESSION   BBa_K301006
VERSION     BBa_K301006.1  
FEATURES             Location/Qualifiers
     misc_feature    4..16
                     /label=IHF
     promoter        126..133
                     /label=-10
     promoter        98..106
                     /label=-35
     misc_feature    92..105
                     /label=Fnr
     misc_feature    88..106
                     /label=Fis
     misc_feature    70..76
                     /label=NarL
     misc_feature    34..45
                     /label=NarL
     misc_feature    54..67
                     /label=Fnr
     misc_feature    57..63
                     /label=NarL
     misc_feature    47..53
                     /label=NarL
     promoter        1..139
                     /label=BBa_K239005
     CDS             146..1145
                     /label=BBa_K301005
     promoter        1154..1176
                     /label=promoter
     promoter        1154..1176
                     /label=BBa_K301003
     CDS             1183..2182
                     /label=BBa_K301005
     promoter        2191..2213
                     /label=promoter
     promoter        2191..2213
                     /label=BBa_K301003
     misc_feature    2226..2229
                     /label=conserved
     RBS             2222..2233
                     /label=BBa_B0034
     CDS             2240..2914
                     /label=mrfp1
     misc_feature    2921..2945
                     /label=Help:Barcodes
     CDS             2240..2920
                     /label=BBa_E1010
     stem_loop       2965..3008
                     /label=stem_loop
     misc_feature    2954..3033
                     /label=BBa_B0010
     terminator      2954..3033
                     /label=BBa_B0010
     stem_loop       3049..3068
                     /label=T7 TE
     misc_feature    3075..3075
                     /label=stop
     polyA_site      3069..3082
                     /label=polya
     misc_feature    3042..3082
                     /label=BBa_B0012
     terminator      3042..3082
                     /label=BBa_B0012
     misc_feature    2222..3082
                     /label=BBa_I13507
ORIGIN
        1 gcaccgtgaa aaatctcata atttttatga agtcactgta ctcactatgg gtaatgataa
       61 atatcaatga tagataaagt tatcttatcg tttgatttac atcaaattgc ctttagctac
      121 agacactaag gtggcagact actagatgaa cacgattaac atcgctaaga acgacttctc
      181 tgacatcgaa ctggctgcta tcccgttcaa cactctggct gaccattacg gtgagcgttt
      241 agctcgcgaa cagttggccc ttgagcatga gtcttacgag atgggtgaag cacgcttccg
      301 caagatgttt gagcgtcaac ttaaagctgg tgaggttgcg gataacgctg ccgccaagcc
      361 tctcatcact accctactcc ctaagatgat tgcacgcatc aacgactggt ttgaggaagt
      421 gaaagctaag cgcggcaagc gcccgacagc cttccagttc ctgcaagaaa tcaagccgga
      481 agccgtagcg tacatcacca ttaagaccac tctggcttgc ctaaccagtg ctgacaatac
      541 aaccgttcag gctgtagcaa gcgcaatcgg tcgggccatt gaggacgagg ctcgcttcgg
      601 tcgtatccgt gaccttgaag ctaagcactt caagaaaaac gttgaggaac aactcaacaa
      661 gcgcgtaggg cacgtctaca agaaagcatt tatgcaagtt gtcgaggctg acatgctctc
      721 taagggtcta ctcggtggcg aggcgtggtc ttcgtggcat aaggaagact ctattcatgt
      781 aggagtacgc tgcatcgaga tgctcattga gtcaaccgga atggttagct tacaccgcca
      841 aaatgctggc gtagtaggtc aagactctga gactatcgaa ctcgcacctg aatacgctga
      901 ggctatcgca acccgtgcag gtgcgctggc tggcatctct ccgatgttcc aaccttgcgt
      961 agttcctcct aagccgtgga ctggcattac tggtggtggc tattgggcta acggtcgtcg
     1021 tcctctggcg ctggtgcgta ctcacagtaa gaaagcactg atgcgctacg aagacgttta
     1081 catgcctgag gtgtacaaag cgattaacat tgcgcaaaac accgcatgga aaatcaacaa
     1141 gaaagtacta gagtaatacg actcactata gggagatact agatgaacac gattaacatc
     1201 gctaagaacg acttctctga catcgaactg gctgctatcc cgttcaacac tctggctgac
     1261 cattacggtg agcgtttagc tcgcgaacag ttggcccttg agcatgagtc ttacgagatg
     1321 ggtgaagcac gcttccgcaa gatgtttgag cgtcaactta aagctggtga ggttgcggat
     1381 aacgctgccg ccaagcctct catcactacc ctactcccta agatgattgc acgcatcaac
     1441 gactggtttg aggaagtgaa agctaagcgc ggcaagcgcc cgacagcctt ccagttcctg
     1501 caagaaatca agccggaagc cgtagcgtac atcaccatta agaccactct ggcttgccta
     1561 accagtgctg acaatacaac cgttcaggct gtagcaagcg caatcggtcg ggccattgag
     1621 gacgaggctc gcttcggtcg tatccgtgac cttgaagcta agcacttcaa gaaaaacgtt
     1681 gaggaacaac tcaacaagcg cgtagggcac gtctacaaga aagcatttat gcaagttgtc
     1741 gaggctgaca tgctctctaa gggtctactc ggtggcgagg cgtggtcttc gtggcataag
     1801 gaagactcta ttcatgtagg agtacgctgc atcgagatgc tcattgagtc aaccggaatg
     1861 gttagcttac accgccaaaa tgctggcgta gtaggtcaag actctgagac tatcgaactc
     1921 gcacctgaat acgctgaggc tatcgcaacc cgtgcaggtg cgctggctgg catctctccg
     1981 atgttccaac cttgcgtagt tcctcctaag ccgtggactg gcattactgg tggtggctat
     2041 tgggctaacg gtcgtcgtcc tctggcgctg gtgcgtactc acagtaagaa agcactgatg
     2101 cgctacgaag acgtttacat gcctgaggtg tacaaagcga ttaacattgc gcaaaacacc
     2161 gcatggaaaa tcaacaagaa agtactagag taatacgact cactataggg agatactaga
     2221 gaaagaggag aaatactaga tggcttcctc cgaagacgtt atcaaagagt tcatgcgttt
     2281 caaagttcgt atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga
     2341 aggtcgtccg tacgaaggta cccagaccgc taaactgaaa gttaccaaag gtggtccgct
     2401 gccgttcgct tgggacatcc tgtccccgca gttccagtac ggttccaaag cttacgttaa
     2461 acacccggct gacatcccgg actacctgaa actgtccttc ccggaaggtt tcaaatggga
     2521 acgtgttatg aacttcgaag acggtggtgt tgttaccgtt acccaggact cctccctgca
     2581 agacggtgag ttcatctaca aagttaaact gcgtggtacc aacttcccgt ccgacggtcc
     2641 ggttatgcag aaaaaaacca tgggttggga agcttccacc gaacgtatgt acccggaaga
     2701 cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg gtcactacga
     2761 cgctgaagtt aaaaccacct acatggctaa aaaaccggtt cagctgccgg gtgcttacaa
     2821 aaccgacatc aaactggaca tcacctccca caacgaagac tacaccatcg ttgaacagta
     2881 cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa cgctgatagt gctagtgtag
     2941 atcgctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     3001 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
     3061 ggtgggcctt tctgcgttta ta
//