BBa_B0011
1
BBa_B0011
LuxICDABEG (+/-)
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Released HQ 2013
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P>
true
Reshma Shetty
annotation1683
1
stem_loop
range1683
1
13
35
annotation7019
1
BBa_B0011
range7019
1
1
46
BBa_K302000
1
BBa_K302000
rocF RBS and coding sequence
2010-08-11T11:00:00Z
2015-05-08T01:11:52Z
''Bacillus subtilis'' 168 genome.
BioBrick-compatible ''rocF'' coding sequence, which codes for the enzyme arginase.
false
false
_442_
0
6009
9
Not in stock
false
Removal of two EcoR1 sites.
false
Alan Koh, Steven Woodhouse, Harsh Sheth
annotation2077659
1
start
range2077659
1
27
29
annotation2077660
1
Double stop
range2077660
1
915
919
annotation2077770
1
Single base mutation to remove EcoR1 site
range2077770
1
227
227
annotation2077771
1
Single base mutation to remove EcoR1 site
range2077771
1
824
824
BBa_B0014
1
BBa_B0014
double terminator (B0012-B0011)
2003-07-15T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0012 and BBa_B0011
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component939303
1
BBa_B0012
component939311
1
BBa_B0011
annotation939311
1
BBa_B0011
range939311
1
50
95
annotation939303
1
BBa_B0012
range939303
1
1
41
BBa_K302014
1
BBa_K302014
IPTG-inducible arginase
2010-08-12T11:00:00Z
2015-05-08T01:11:52Z
The ''rocF'' sequence was taken from ''Bacillus subtilis'' 168 genomic DNA, by PCR. Pspac oid,. .... assembled by the Gibson method...
IPTG-inducible arginase production for ''Bacillus subtilis''.
false
false
_442_
0
6009
9
Not in stock
true
Two EcoR1 restriction sites were removed from the ''rocF'' coding sequence.
false
Alan Koh, Steven Woodhouse, Harsh Sheth
component2220643
1
BBa_B0014
component2220636
1
BBa_K302000
component2220631
1
BBa_K174004
annotation2220636
1
BBa_K302000
range2220636
1
107
1036
annotation2220631
1
BBa_K174004
range2220631
1
1
106
annotation2220643
1
BBa_B0014
range2220643
1
1037
1131
BBa_K174004
1
BBa_K174004
Pspac promoter
2009-10-09T11:00:00Z
2015-05-08T01:10:58Z
This sequence is taken from pmutin4 integration vector. pmutin2's lacI binding site was changed with the "oid" sequence to create pmutin4.
This promoter can be induced by IPTG. Wild type LacI binding site is replaced with a perfect palindromic "oid" operator sequence which increases the repression by LacI.
This biobrick is an enhancement of Biobrick BBa_K090501 which has the sequence from pmutin2.
false
false
_277_
0
3942
9
Not in stock
false
pmutin2's genbank accession number is AF072806. Although there is no sequence information for pmutin4, it can be derived from pmutin2's sequence information by changing the O1 lacI binding site with the "oid" sequence.
false
The Newcastle 2009 iGEM team
annotation2033093
1
oid - Lac operator
range2033093
1
81
100
annotation2033094
1
pspac promoter
range2033094
1
43
80
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
BBa_K174004_sequence
1
agaacaacctctgctaaaattcctgaaaaattttgcaaaaagttgttgactttatctacaaggtgtggcataatgtgtggaattgtgagcgctcacaattaagctt
BBa_B0014_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_K302014_sequence
1
agaacaacctctgctaaaattcctgaaaaattttgcaaaaagttgttgactttatctacaaggtgtggcataatgtgtggaattgtgagcgctcacaattaagctttcttaaatgttgaggtggaatcacagatggataaaacgatttcggttattggaatgccaatggatttaggacaagcacgacgcggagtggatatgggcccgagtgccatccggtacgctcatctgatcgagaggctgtcagacatggggtatacggttgaagatctcggtgacattccgatcaatcgcgaaaaaatcaaaaatgacgaggaactgaaaaacctgaactccgttttggcgggaaatgaaaaactcgcgcaaaaggtcaacaaagtcattgaagagaaaaaattcccgcttgtcctgggcggtgaccacagtattgcgatcggcacgcttgcaggcacagcgaagcattacgataatctcggcgtcatctggtatgacgcgcacggcgatttgaatacacttgaaacttcaccatcgggcaatattcacggcatgccgctcgcggtcagcctaggcattggccacgagtcactggttaaccttgaaggctacgcgcctaaaatcaaaccggaaaacgtcgtcatcattggcgcccggtcacttgatgaaggggagcgcaagtacattaaggaaagcggcatgaaggtgtacacaatgcacgaaatcgatcgtcttggcatgacaaaggtcattgaagaaacccttgattatttatcagcatgtgatggcgtccatctgagccttgatctggacggacttgatccgaacgacgcaccgggtgtcggaacccctgtcgtcggcggcatcagctaccgggagagccatttggctatggaaatgctgtatgacgcaggcatcattacctcagccgaatttgttgaggttaacccgatccttgatcacaaaaacaaaacgggcaaaacagcagtagagctcgtagaatccctgttagggaagaagctgctgtaataagaaaaccccctcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0011_sequence
1
agagaatataaaaagccagattattaatccggcttttttattattt
BBa_K302000_sequence
1
tcttaaatgttgaggtggaatcacagatggataaaacgatttcggttattggaatgccaatggatttaggacaagcacgacgcggagtggatatgggcccgagtgccatccggtacgctcatctgatcgagaggctgtcagacatggggtatacggttgaagatctcggtgacattccgatcaatcgcgaaaaaatcaaaaatgacgaggaactgaaaaacctgaactccgttttggcgggaaatgaaaaactcgcgcaaaaggtcaacaaagtcattgaagagaaaaaattcccgcttgtcctgggcggtgaccacagtattgcgatcggcacgcttgcaggcacagcgaagcattacgataatctcggcgtcatctggtatgacgcgcacggcgatttgaatacacttgaaacttcaccatcgggcaatattcacggcatgccgctcgcggtcagcctaggcattggccacgagtcactggttaaccttgaaggctacgcgcctaaaatcaaaccggaaaacgtcgtcatcattggcgcccggtcacttgatgaaggggagcgcaagtacattaaggaaagcggcatgaaggtgtacacaatgcacgaaatcgatcgtcttggcatgacaaaggtcattgaagaaacccttgattatttatcagcatgtgatggcgtccatctgagccttgatctggacggacttgatccgaacgacgcaccgggtgtcggaacccctgtcgtcggcggcatcagctaccgggagagccatttggctatggaaatgctgtatgacgcaggcatcattacctcagccgaatttgttgaggttaacccgatccttgatcacaaaaacaaaacgggcaaaacagcagtagagctcgtagaatccctgttagggaagaagctgctgtaataagaaaaccccc
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z