BBa_B0011
1
BBa_B0011
LuxICDABEG (+/-)
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Released HQ 2013
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P>
true
Reshma Shetty
annotation7019
1
BBa_B0011
range7019
1
1
46
annotation1683
1
stem_loop
range1683
1
13
35
BBa_K302010
1
BBa_K302010
sfp
2010-08-11T11:00:00Z
2015-05-08T01:11:52Z
''Bacillus subtilis'' 3610 genomic DNA, by PCR.
''Bacillus subtilis'' 168 is unable to produce surfactin, a natural surfactant which helps in reducing surface tension and aids bacteria to swarm on a solid or semi solid medium. The ''sfp'' gene restores the capability to produce surfactin.
false
false
_442_
0
6009
9
Not in stock
false
Second TAA stop codon was added.
false
Harsh Sheth, Alan Koh, Steven Woodhouse
annotation2080974
1
Added TAA
range2080974
1
676
678
BBa_K143012
1
Pveg
Promoter veg a constitutive promoter for B. subtilis
2008-09-10T11:00:00Z
2015-05-08T01:10:23Z
The Pveg promoter was suggested to us by Dr. Jan-Willem Veening of Newcastle University. This sequence supplied was compared to that of the DBTBS database<cite>#3</cite> then a section containing the binding site synthesised by Geneart.
Released HQ 2013
Pveg is a constitutive promoter that constitutively expresses the P43 protein in ''B.subtilis''. Pveg contains binding sites for the ''B.sutbilis'' major sigma factor<cite>#1</cite>. Pveg in ''B.subtilis'' utilises two binding sites to cause high expression of genes<cite>#2</cite>, however our Pveg is lacking the upstream site to give a medium level of gene expression. It has been noted that the sporulation master regulatoion factor spoOA interacts with Pveg though it is not known how<cite>#3</cite>. The context with which we used the promoter Pveg is as a '''Polymerase Per Second''' (PoPS) generator.
false
true
_199_
0
2090
9
In stock
false
The biobrick part was designed to include a single binding site for the ''B.subtilis major sigma factor. In addition the biobrick standard was applied to the promoter Pveg sequence.
false
James Chappell
annotation1975704
1
Sigma A-35
range1975704
1
63
68
annotation1975705
1
Sigma A -10
range1975705
1
86
91
BBa_K302011
1
BBa_K302011
swrA
2010-08-11T11:00:00Z
2015-05-08T01:11:52Z
''Bacillius subtilis'' 3610 genomic DNA, by PCR.
''Bacillus subtilis'' 168 is unable to biosynthesise flagella. The ''swrA'' gene restores this capability.
false
false
_442_
0
6009
9
Not in stock
false
Extra TAA stop codon added.
false
Harsh Sheth, Alan Koh, Steven Woodhouse
annotation2077615
1
Added TAA
range2077615
1
430
432
BBa_K143053
1
Pveg-spoVG
Promoter Pveg and RBS spoVG for B. subtilis
2008-10-07T11:00:00Z
2015-05-08T01:10:24Z
Pveg-spoVG was synthesised by GeneArt
Released HQ 2013
Constitutive promoter veg(<bbpart>BBa_K143012</bbpart>) coupled to the strong Ribosome Binding Site spoVG(<bbpart>BBa_K143021</bbpart>) from ''B. subtilis''.
Pveg-spoVG can be used in the context of a '''Ribosomes per second''' (RiPS) output generator
'''To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix<cite>1</cite>. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.'''
false
true
_199_
0
3475
9
In stock
false
The sequence of Pveg was obtained from the DBTBS<cite>1</cite> and RBS-spoVG were obtained from papers<cite>2</cite> and the sequence synthesised by GeneArt
true
Chris Hirst
component1979397
1
BBa_K143021
component1979395
1
BBa_K143012
annotation1979395
1
BBa_K143012
range1979395
1
1
97
annotation1979397
1
BBa_K143021
range1979397
1
106
117
BBa_B0014
1
BBa_B0014
double terminator (B0012-B0011)
2003-07-15T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0012 and BBa_B0011
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component939311
1
BBa_B0011
component939303
1
BBa_B0012
annotation939311
1
BBa_B0011
range939311
1
50
95
annotation939303
1
BBa_B0012
range939303
1
1
41
BBa_K143021
1
RBS-spoVG
SpoVG ribosome binding site (RBS) for B. subtilis
2008-09-16T11:00:00Z
2015-05-08T01:10:23Z
The sequence was taken from a previous research paper [1] and was constructed by Geneart.
Released HQ 2013
Description: SpoVG is an endogenous ribosome binding site from B.subtilis. The sequence of the spoVG ribosome binding site is AAAGGUGGUGA which is complementary to the sequence UUUCCUCCACU from the 3' region of the 16s rRNA from B.subtilis. Previous research showed that the predicted binding energy of the 16s rRNA to the RBS is -19kcal <cite>1</cite>
false
true
_199_
0
2090
9
In stock
false
In order to ensure that the RBS is functional the actual ribosome binding site was maintained and the distance between the RBS and the start codon maintained. In order to conform to the biobrick standard the sequence flanking the RBS had to be changed but the distance between the promoter and RBS, and start codon and RBS was maintained.
false
James Chappell
annotation1975997
1
rbs
range1975997
1
1
12
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
BBa_K302016
1
BBa_K302016
Swarming
2010-08-11T11:00:00Z
2015-05-08T01:11:52Z
Composite
Swarming
false
false
_442_
0
6009
9
Not in stock
true
None.
false
Harsh Sheth, Alan Koh, Steven Woodhouse
component2227547
1
BBa_B0014
component2227532
1
BBa_K143053
component2227537
1
BBa_K143021
component2227535
1
BBa_K302010
component2227540
1
BBa_K302011
component2227538
1
BBa_G0000
component2227533
1
BBa_G0000
annotation2227533
1
BBa_G0000
range2227533
1
118
123
annotation2227547
1
BBa_B0014
range2227547
1
1252
1346
annotation2227537
1
BBa_K143021
range2227537
1
802
813
annotation2227535
1
BBa_K302010
range2227535
1
124
801
annotation2227532
1
BBa_K143053
range2227532
1
1
117
annotation2227540
1
BBa_K302011
range2227540
1
820
1251
annotation2227538
1
BBa_G0000
range2227538
1
814
819
BBa_G0000
1
scar
SpeI/XbaI scar for RBS-CDS junctions
2007-07-22T11:00:00Z
2015-08-31T04:07:27Z
SpeI/XbaI scar
This is the sequence of the SpeI/XbaI scar for RBS-CDS junctions in BioBricks standard assembly.
false
true
_41_
0
126
162
Not in stock
false
This is a shorter scar to ensure proper spacing between the RBS and CDS.
false
Reshma Shetty
BBa_K143053_sequence
1
aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagaaaggtggtgaa
BBa_B0014_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_K143021_sequence
1
aaaggtggtgaa
BBa_G0000_sequence
1
tactag
BBa_K302011_sequence
1
atgcttgttagcactccagtactgctgcttatactggcccgtgcttatctgtataaagaaaatgggggttcacaattgaagagggcaagtattgtgcgtgaaaaaaaatattatgaattagtggaacaattaaaagacagaacacaagacgtaacattttcagctacaaaagcactaagtcttcttatgctgttcagcagatatttggtcaattacaccaatgtcgaatcagtaaatgacattaatgaggaatgcgccaaacattattttaactacttaatgaaaaaccataagcgattaggaattaatctgacagatataaaaaggtcgatgcatctaatcagcgggttattggatgtggatgtaaaccactatttaaaggatttttcactatcgaatgtcacgctgtggatgacgcaagagagataataa
BBa_K143012_sequence
1
aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgt
BBa_B0011_sequence
1
agagaatataaaaagccagattattaatccggcttttttattattt
BBa_K302016_sequence
1
aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagaaaggtggtgaatactagatgaagatttacggaatttatatggaccgcccgctttcacaggaagaaaatgaacggttcatgtctttcatatcacctgaaaaacgggagaaatgccggagattttatcataaagaagatgctcaccgcaccctgctgggagatgtgctcgttcgctcagtcataagcaggcagtatcagttggacaaatccgatatccgctttagcacgcaggaatacgggaagccgtgcatccctgatcttcccgacgctcatttcaacatttctcactccggacgctgggtcatttgcgcgtttgattcacagccgatcggcatagatatcgaaaaaacgaaaccgatcagccttgagatcgccaagcgcttcttttcaaaaacagagtacagcgaccttttagcaaaagacaaggacgagcagacagactatttttatcatctatggtcaatgaaagaaagctttatcaaacaggaaggcaaaggcttatcgcttccgcttgattccttttcagtgcgcctgcaccaggacggacaagtatccattgagcttccggacagccattccccatgctatatcaaaacgtatgaggtcgatcccggctacaaaatggctgtatgcgccgtacaccctgatttccccgaggatatcacaatggtctcgtacgaagagcttttataataaaaaggtggtgaatactagatgcttgttagcactccagtactgctgcttatactggcccgtgcttatctgtataaagaaaatgggggttcacaattgaagagggcaagtattgtgcgtgaaaaaaaatattatgaattagtggaacaattaaaagacagaacacaagacgtaacattttcagctacaaaagcactaagtcttcttatgctgttcagcagatatttggtcaattacaccaatgtcgaatcagtaaatgacattaatgaggaatgcgccaaacattattttaactacttaatgaaaaaccataagcgattaggaattaatctgacagatataaaaaggtcgatgcatctaatcagcgggttattggatgtggatgtaaaccactatttaaaggatttttcactatcgaatgtcacgctgtggatgacgcaagagagataataatcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K302010_sequence
1
atgaagatttacggaatttatatggaccgcccgctttcacaggaagaaaatgaacggttcatgtctttcatatcacctgaaaaacgggagaaatgccggagattttatcataaagaagatgctcaccgcaccctgctgggagatgtgctcgttcgctcagtcataagcaggcagtatcagttggacaaatccgatatccgctttagcacgcaggaatacgggaagccgtgcatccctgatcttcccgacgctcatttcaacatttctcactccggacgctgggtcatttgcgcgtttgattcacagccgatcggcatagatatcgaaaaaacgaaaccgatcagccttgagatcgccaagcgcttcttttcaaaaacagagtacagcgaccttttagcaaaagacaaggacgagcagacagactatttttatcatctatggtcaatgaaagaaagctttatcaaacaggaaggcaaaggcttatcgcttccgcttgattccttttcagtgcgcctgcaccaggacggacaagtatccattgagcttccggacagccattccccatgctatatcaaaacgtatgaggtcgatcccggctacaaaatggctgtatgcgccgtacaccctgatttccccgaggatatcacaatggtctcgtacgaagagcttttataataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z