LOCUS       BBa_K305011             3162 bp    DNA     linear       01-Jun-2024
DEFINITION  Subtilin inducible expression plasmid for Bacillus subtilis
ACCESSION   BBa_K305011
VERSION     BBa_K305011.1  
FEATURES             Location/Qualifiers
     misc_feature    1..21
                     /label=BioBrick suffix
     misc_feature    3141..3162
                     /label=BioBrick prefix
     CDS             1158..1856
                     /label=repA
     CDS             2315..2965
                     /label=Chloramphenicol resistance
     CDS             681..890
                     /label=repC
     promoter        3061..3138
                     /label=spaS
     stem_loop       103..155
                     /label=Terminator
     primer_bind     2925..2944
                     /label=Brick sequencing primer (3CF) binding site
     primer_bind     60..78
                     /label=Brick sequencing primer (3BR) binding site
     misc_feature    3163..3168
                     /label=BamHI site for circularization
ORIGIN
        1 tactagtagc ggccgctgca gcaaattaca gcacgtgttg ctttgattga tagccaaaaa
       61 gcagcagttg ataaagcaat tactgatatt gctgaaaaat tgtaatttat aaataaaaat
      121 caccttttag aggtggtttt tttatttata aattattcgt ttgatttcgc tttcgataga
      181 acaatcaaag cgagaataag gaagataaat cccataaggg cgggagcaga atgtccgaga
      241 ctaatgtaaa tttgtccacc aattaaagga ccgataacgc gagcttctcg agtgcatatt
      301 ttcggcaatc ttctcaatga gatgctcttc agcatgttca atgatgtcga ttttttatta
      361 aaacgtctca aaatcgtttc tgagacgttt tagcgtttat ttcgtttagt tatcggcata
      421 atcgttaaaa caggcgttat cgtagcgtaa aagcccttga gcgtagcgtg ctttgcagcg
      481 aagatgttgt ctgttagatt atgaaagccg atgactgaat gaaataataa gcgcagcgtc
      541 cttctatttc ggttggagga ggctcaaggg agtttgaggg aatgaaattc cctcatgggt
      601 ttgattttaa aaattgcttg caattttgcc gagcggtagc gctggaaaat ttttgaaaaa
      661 aatttggaat ttggaaaaaa atggggggaa aggaagcgaa ttttgcttcc gtactacgac
      721 cccccattaa gtgccgagtg ccaatttttg tgccaaaaac gctctatccc aactggctca
      781 agggtttgag gggtttttca atcgccaacg aatcgccaac gttttcgcca acgtttttta
      841 taaatctata tttaagtagc tttattgttg tttttatgat tacaaagtga tacactaatt
      901 ttataaaatt atttgattgg agttttttaa atggtgattt cagaatcgaa aaaaagagtt
      961 atgatttctc tgacaaaaga gcaagataaa aaattaacag atatggcgaa acaaaaaggt
     1021 ttttcaaaat ctgcggttgc ggcgttagct atagaagaat atgcaagaaa ggaatcagaa
     1081 caaaaaaaat aagcgaaagc tcgcgttttt agaaggatac gagttttcgc tacttgtttt
     1141 tgataaggta atatatcatg gctattaaaa atactaaagc tagaaatttt ggatttttat
     1201 tatatcctga ctcaattcct aatgattgga aagaaaaatt agagagtttg ggcgtatcta
     1261 tggctgtcag tcctttacac gatatggacg aaaaaaaaga taaagataca tggaatagta
     1321 gtgatgttat acgaaatgga aagcactata aaaaaccaca ctatcacgtt atatatattg
     1381 cacgaaatcc tgtaacaata gaaagcgtta ggaacaagat taagcgaaaa ttggggaata
     1441 gttcagttgc tcatgttgag atacttgatt atatcaaagg ttcatatgaa tatttgactc
     1501 atgaatcaaa ggacgctatt gctaagaata aacatatata cgacaaaaaa gatattttga
     1561 acattaatga ttttgatatt gaccgctata taacacttga tgaaagccaa aaaagagaat
     1621 tgaagaattt acttttagat atagtggatg actataattt ggtaaataca aaagatttaa
     1681 tggcttttat tcgccttagg ggagcggagt ttggaatttt aaatacgaat gatgtaaaag
     1741 atattgtttc aacaaactct agcgccttta gattatggtt tgagggcaat tatcagtgtg
     1801 gatatagagc aagttatgca aaggttcttg atgctgaaac gggggaaata aaatgacaaa
     1861 caaagaaaaa gagttatttg ctgaaaatga ggaattaaaa aaagaaatta aggacttaaa
     1921 agagcgtatt gaaagataca gagaaatgga agttgaatta agtacaacaa tagatttatt
     1981 gagaggaggg attattgaat aaataaaagc ccccctgacg aaagtcgacg gcaatagtta
     2041 cccttattat caagataaga aagaaaagga tttttcgcta cgctcaaatc ctttaaaaaa
     2101 acacaaaaga ccacattttt taatgtggtc tttattcttc aactaaagca cccattagtt
     2161 caacaaacga aaattggata aagtgggata tttttaaaat atatatttat gttacagtaa
     2221 tattgacttt taaaaaagga ttgattctaa tgaagaaagc agacaagtaa gcctcctaaa
     2281 ttcactttag ataaaaattt aggaggcata tcaaatgaac tttaataaaa ttgatttaga
     2341 caattggaag agaaaagaga tatttaatca ttatttgaac caacaaacga cttttagtat
     2401 aaccacagaa attgatatta gtgttttata ccgaaacata aaacaagaag gatataaatt
     2461 ttaccctgca tttattttct tagtgacaag ggtgataaac tcaaatacag cttttagaac
     2521 tggttacaat agcgacggag agttaggtta ttgggataag ttagagccac tttatacaat
     2581 ttttgatggt gtatctaaaa cattctctgg tatttggact cctgtaaaga atgacttcaa
     2641 agagttttat gatttatacc tttctgatgt agagaaatat aatggttcgg ggaaattgtt
     2701 tcccaaaaca cctatacctg aaaatgcttt ttctctttct attattcctt ggacttcatt
     2761 tactgggttt aacttaaata tcaataataa tagtaattac cttctaccca ttattacagc
     2821 aggaaaattc attaataaag gtaattcaat atatttaccg ctatctttac aggtacatca
     2881 ttctgtttgt gatggttatc atgcaggatt gtttatgaac tctattcagg aattgtcaga
     2941 taggcctaat gactggcttt tataatatga gataatgccg actgtacttt ttacagtcgg
     3001 ttttctaatg tcactaacct gccccgttag ttgaagaagg tttttatatt acagctccaa
     3061 gatcttaaaa aaaggaaaaa attgataaaa tcttgatatt tgtctgttac tatttaggta
     3121 ttgaaaggag gtgaccacca gaattcgcgg ccgcttctag ag
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