LOCUS       BBa_K316008             1948 bp    DNA     linear       20-May-2024
DEFINITION  Cleavable GFP-XylE fusion with Pveg promoter and terminator
ACCESSION   BBa_K316008
VERSION     BBa_K316008.1  
FEATURES             Location/Qualifiers
     promoter        63..68
                     /label=Sigma A-35
     promoter        86..91
                     /label=Sigma A -10
     promoter        1..97
                     /label=BBa_K143012
     RBS             106..117
                     /label=rbs
     RBS             106..117
                     /label=BBa_K143021
     misc_feature    1..117
                     /label=BBa_K143053
     misc_feature    127..144
                     /label=His tag
     misc_feature    124..126
                     /label=Start
     CDS             145..855
                     /label=GFP E0040
     misc_feature    856..879
                     /label=Flag Tag
     misc_feature    880..900
                     /label=Tev Protease Cleavage Site
     misc_feature    901..918
                     /label=GGS linker
     CDS             919..1845
                     /label=XylE J33204
     misc_feature    124..1845
                     /label=BBa_K316006
     stem_loop       1861..1880
                     /label=T7 TE
     misc_feature    1887..1887
                     /label=stop
     polyA_site      1881..1894
                     /label=polya
     misc_feature    1854..1894
                     /label=BBa_B0012
     terminator      1854..1894
                     /label=BBa_B0012
     stem_loop       1915..1937
                     /label=stem_loop
     misc_feature    1903..1948
                     /label=BBa_B0011
     terminator      1903..1948
                     /label=BBa_B0011
     terminator      1854..1948
                     /label=BBa_B0014
ORIGIN
        1 aattttgtca aaataatttt attgacaacg tcttattaac gttgatataa tttaaatttt
       61 atttgacaaa aatgggctcg tgttgtacaa taaatgttac tagagaaagg tggtgaatac
      121 tagatgcatc accatcatca ccatcgtaaa ggagaagaac ttttcactgg agttgtccca
      181 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
      241 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
      301 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga
      361 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta
      421 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
      481 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
      541 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
      601 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat
      661 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
      721 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
      781 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
      841 gatgaactat acaaagatta taaagatgat gacgacaagg aaaatcttta ttttcaaggg
      901 ggaggttcag gaggcagcat gaacaaaggt gtaatgcgac cgggccatgt gcagctgcgt
      961 gtactggaca tgagcaaggc cctggaacac tacgtcgagt tgctgggcct gatcgagatg
     1021 gaccgtgacg accagggccg tgtctatctg aaggcttgga ccgaagtgga taagttttcc
     1081 ctggtgctac gcgaggctga cgagccgggc atggatttta tgggtttcaa ggttgtggat
     1141 gaggatgctc tccggcaact ggagcgggat ctgatggcat atggctgtgc cgttgagcag
     1201 ctacccgcag gtgaactgaa cagttgtggc cggcgcgtgc gcttccaggc cccctccggg
     1261 catcacttcg agttgtatgc agacaaggaa tatactggaa agtggggttt gaatgacgtc
     1321 aatcccgagg catggccgcg cgatctgaaa ggtatggcgg ctgtgcgttt cgaccacgcc
     1381 ctcatgtatg gcgacgaatt gccggcgacc tatgacctgt tcaccaaggt gctcggtttc
     1441 tatctggccg aacaggtgct ggacgaaaat ggcacgcgcg tcgcccagtt tctcagtctg
     1501 tcgaccaagg cccacgacgt ggccttcatt caccatccgg aaaaaggccg cctccatcat
     1561 gtgtccttcc acctcgaaac ctgggaagac ttgcttcgcg ccgccgacct gatctccatg
     1621 accgacacat ctatcgatat cggcccaacc cgccacggcc tcactcacgg caagaccatc
     1681 tacttcttcg acccgtccgg taaccgcaac gaagtgttct gcgggggaga ttacaactac
     1741 ccggaccaca aaccggtgac ctggaccacc gaccagctgg gcaaggcgat cttttaccac
     1801 gaccgcattc tcaacgaacg attcatgacc gtgctgacct aataatacta gagtcacact
     1861 ggctcacctt cgggtgggcc tttctgcgtt tatatactag agagagaata taaaaagcca
     1921 gattattaat ccggcttttt tattattt
//