LOCUS       BBa_K316009             2830 bp    DNA     linear       21-May-2024
DEFINITION  LacI inducible Fast Response Module, using cleavable XylE
ACCESSION   BBa_K316009
VERSION     BBa_K316009.1  
FEATURES             Location/Qualifiers
     CDS             1..717
                     /label=TEV protease
     CDS             1..717
                     /label=BBa_K316012
     RBS             726..737
                     /label=sRBS
     misc_feature    738..745
                     /label=scar
     misc_feature    746..776
                     /label=LacI binding
     misc_feature    836..865
                     /label=LacI binding
     promoter        726..874
                     /label=BBa_K316000
     CDS             1..874
                     /label=BBa_K316017
     promoter        945..950
                     /label=Sigma A-35
     promoter        968..973
                     /label=Sigma A -10
     promoter        883..979
                     /label=BBa_K143012
     RBS             988..999
                     /label=rbs
     RBS             988..999
                     /label=BBa_K143021
     misc_feature    883..999
                     /label=BBa_K143053
     misc_feature    1009..1026
                     /label=His tag
     misc_feature    1006..1008
                     /label=Start
     CDS             1027..1737
                     /label=GFP E0040
     misc_feature    1738..1761
                     /label=Flag Tag
     misc_feature    1762..1782
                     /label=Tev Protease Cleavage Site
     misc_feature    1783..1800
                     /label=GGS linker
     CDS             1801..2727
                     /label=XylE J33204
     misc_feature    1006..2727
                     /label=BBa_K316006
     stem_loop       2743..2762
                     /label=T7 TE
     misc_feature    2769..2769
                     /label=stop
     polyA_site      2763..2776
                     /label=polya
     misc_feature    2736..2776
                     /label=BBa_B0012
     terminator      2736..2776
                     /label=BBa_B0012
     stem_loop       2797..2819
                     /label=stem_loop
     misc_feature    2785..2830
                     /label=BBa_B0011
     terminator      2785..2830
                     /label=BBa_B0011
     terminator      2736..2830
                     /label=BBa_B0014
ORIGIN
        1 ttattagttc attaactgtg tggcctcttt aaccggttga aacggttctt ccggtttatc
       61 catgaacact ttgtgacctc cccaaagaac ggaatctgca ttcagtctcc agcctgaaac
      121 ccattgttga gcttcttggt tcgtcagtaa ctccatgaag ttcttgggaa cactcgtaaa
      181 gtaattattc gtattggtga agtttgacgc agagtgtatg cccacaataa acccatcccg
      241 tgttgacaca agaggagagc cacattgccc atcttttgtc tgaatccaat gcttccaaaa
      301 gatgccatca gagctaggga atgtacagct agtgtctgat accattgagg acatagattt
      361 ggtctgaaag tttgttgtga ctaagcagat ccgttcttca cgctgaggtt cgcgaaactt
      421 gagtttctga ggaaacggtg gaaagtcttt cggcattcga atgataatca tgtctcttcc
      481 atctatcaga tgttgctgaa gcgtcgttgt gtttttgacc ttaaagactc catgaaggga
      541 ttgtaccaac agtgtaccat tattgcgcct aaagaggtgt ttgttcgtga tgatgaatgg
      601 cccaaagcca atgccataca agctagtggt atgaccatca ctctcattgg tcagatggca
      661 aatagtcgag ctaatcggat tatagtcacg tggtccttta aacaacgatc ttcccattac
      721 tagagttcac ctcctttctc tagtatgtga attgttatcc gctcacaatt ccacacacac
      781 attatgccac accttgtaga taaagtcaac aacttttgca actttctcgg caaaatgtgg
      841 aattgtgagc gctcacaatt ccacaaccct cgagtactag agaattttgt caaaataatt
      901 ttattgacaa cgtcttatta acgttgatat aatttaaatt ttatttgaca aaaatgggct
      961 cgtgttgtac aataaatgtt actagagaaa ggtggtgaat actagatgca tcaccatcat
     1021 caccatcgta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
     1081 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac
     1141 ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca
     1201 cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa
     1261 cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt
     1321 ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt
     1381 gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac
     1441 aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat
     1501 ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca
     1561 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat
     1621 tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc
     1681 cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaagat
     1741 tataaagatg atgacgacaa ggaaaatctt tattttcaag ggggaggttc aggaggcagc
     1801 atgaacaaag gtgtaatgcg accgggccat gtgcagctgc gtgtactgga catgagcaag
     1861 gccctggaac actacgtcga gttgctgggc ctgatcgaga tggaccgtga cgaccagggc
     1921 cgtgtctatc tgaaggcttg gaccgaagtg gataagtttt ccctggtgct acgcgaggct
     1981 gacgagccgg gcatggattt tatgggtttc aaggttgtgg atgaggatgc tctccggcaa
     2041 ctggagcggg atctgatggc atatggctgt gccgttgagc agctacccgc aggtgaactg
     2101 aacagttgtg gccggcgcgt gcgcttccag gccccctccg ggcatcactt cgagttgtat
     2161 gcagacaagg aatatactgg aaagtggggt ttgaatgacg tcaatcccga ggcatggccg
     2221 cgcgatctga aaggtatggc ggctgtgcgt ttcgaccacg ccctcatgta tggcgacgaa
     2281 ttgccggcga cctatgacct gttcaccaag gtgctcggtt tctatctggc cgaacaggtg
     2341 ctggacgaaa atggcacgcg cgtcgcccag tttctcagtc tgtcgaccaa ggcccacgac
     2401 gtggccttca ttcaccatcc ggaaaaaggc cgcctccatc atgtgtcctt ccacctcgaa
     2461 acctgggaag acttgcttcg cgccgccgac ctgatctcca tgaccgacac atctatcgat
     2521 atcggcccaa cccgccacgg cctcactcac ggcaagacca tctacttctt cgacccgtcc
     2581 ggtaaccgca acgaagtgtt ctgcggggga gattacaact acccggacca caaaccggtg
     2641 acctggacca ccgaccagct gggcaaggcg atcttttacc acgaccgcat tctcaacgaa
     2701 cgattcatga ccgtgctgac ctaataatac tagagtcaca ctggctcacc ttcgggtggg
     2761 cctttctgcg tttatatact agagagagaa tataaaaagc cagattatta atccggcttt
     2821 tttattattt
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