LOCUS       BBa_K316022             2547 bp    DNA     linear       10-May-2024
DEFINITION  B.subtilis transformation vector, targets amyE locus
ACCESSION   BBa_K316022
VERSION     BBa_K316022.1  
FEATURES             Location/Qualifiers
     misc_feature    1..522
                     /label=5' AmyE homologous sequence
     misc_feature    1..522
                     /label=BBa_K143001
     misc_feature    531..558
                     /label=BBa_K316002
     promoter        629..634
                     /label=Sigma A-35
     promoter        652..657
                     /label=Sigma A -10
     promoter        567..663
                     /label=BBa_K143012
     RBS             673..679
                     /label=rbs
     RBS             672..682
                     /label=BBa_K143020
     misc_feature    567..682
                     /label=BBa_K143052
     misc_feature    689..1348
                     /label=CmR gene
     CDS             689..1348
                     /label=BBa_J31005
     stem_loop       1368..1411
                     /label=stem_loop
     misc_feature    1357..1436
                     /label=BBa_B0010
     terminator      1357..1436
                     /label=BBa_B0010
     stem_loop       1452..1471
                     /label=T7 TE
     misc_feature    1478..1478
                     /label=stop
     polyA_site      1472..1485
                     /label=polya
     misc_feature    1445..1485
                     /label=BBa_B0012
     terminator      1445..1485
                     /label=BBa_B0012
     misc_feature    689..1485
                     /label=BBa_K143064
     misc_feature    1494..1521
                     /label=BBa_K316002
     misc_feature    1530..1533
                     /label=5' product
     misc_feature    1534..1537
                     /label=3' product
     misc_feature    1530..1537
                     /label=BBa_K316013
     misc_feature    1494..1537
                     /label=BBa_K316014
     misc_feature    1546..2550
                     /label=3' AmyE homologous sequence
     misc_feature    1546..2547
                     /label=BBa_K143002
ORIGIN
        1 atgtttgcaa aacgattcaa aacctcttta ctgccgttat tcgctggatt tttattgctg
       61 tttcatttgg ttctggcagg accggcggct gcgagtgctg aaacggcgaa caaatcgaat
      121 gagcttacag caccgtcgat caaaagcgga accattcttc atgcatggaa ttggtcgttc
      181 aatacgttaa aacacaatat gaaggatatt catgatgcag gatatacagc cattcagaca
      241 tctccgatta accaagtaaa ggaagggaat caaggagata aaagcatgtc gaactggtac
      301 tggctgtatc agccgacatc gtatcaaatt ggcaaccgtt acttaggtac tgaacaagaa
      361 tttaaagaaa tgtgtgcagc cgctgaagaa tatggcataa aggtcattgt tgacgcggtc
      421 atcaatcata ccaccagtga ttatgccgcg atttccaatg aggttaagag tattccaaac
      481 tggacacatg gaaacacaca aattaaaaac tggtctgatc gatactagag atctcctaga
      541 atatatatta tgtaaactta ctagagaatt ttgtcaaaat aattttattg acaacgtctt
      601 attaacgttg atataattta aattttattt gacaaaaatg ggctcgtgtt gtacaataaa
      661 tgttactaga gtaaaggagg aatactagat ggagaaaaaa atcactggat ataccaccgt
      721 tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg
      781 tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa
      841 taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc
      901 ggaatttcgt atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg
      961 ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga
     1021 cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct
     1081 ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt
     1141 gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt
     1201 caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt
     1261 tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta
     1321 ctgcgatgag tggcagggcg gggcgtaata ctagagccag gcatcaaata aaacgaaagg
     1381 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     1441 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagatctcct
     1501 agaatatata ttatgtaaac ttactagagg aaatttctac tagagatccg tttaggctgg
     1561 gcggtgatag cttctcgttc aggcagtacg cctcttttct tttccagacc tgagggaggc
     1621 ggaaatggtg tgaggttccc ggggaaaagc caaataggcg atcgcgggag tgctttattt
     1681 gaagatcagg ctatcactgc ggtcaataga tttcacaatg tgatggctgg acagcctgag
     1741 gaactctcga acccgaatgg aaacaaccag atatttatga atcagcgcgg ctcacatggc
     1801 gttgtgctgg caaatgcagg ttcatcctct gtctctatca atacggcaac aaaattgcct
     1861 gatggcaggt atgacaataa agctggagcg ggttcatttc aagtgaacga tggtaaactg
     1921 acaggcacga tcaatgccag gtctgtagct gtgctttatc ctgatgatat tgcaaaagcg
     1981 cctcatgttt tccttgagaa ttacaaaaca ggtgtaacac attctttcaa tgatcaactg
     2041 acgattacct tgcgtgcaga tgcgaataca acaaaagccg tttatcaaat caataatgga
     2101 ccagagacgg cgtttaagga tggagatcaa ttcacaatcg gaaaaggaga tccatttggc
     2161 aaaacataca ccatcatgtt aaaaggaacg aacagtgatg gtgtaacgag gaccgagaaa
     2221 tacagttttg ttaaaagaga tccagcgtcg gccaaaacca tcggctatca aaatccgaat
     2281 cattggagcc aggtaaatgc ttatatctat aaacatgatg ggagccgagt aattgaattg
     2341 accggatctt ggcctggaaa accaatgact aaaaatgcag acggaattta cacgctgacg
     2401 ctgcctgcgg acacggatac aaccaacgca aaagtgattt ttaataatgg cagcgcccaa
     2461 gtgcccggtc agaatcagcc tggctttgat tacgtgctaa atggtttata taatgactcg
     2521 ggcttaagcg gttctcttcc ccattga
//