LOCUS       BBa_K316024             1381 bp    DNA     linear       10-May-2024
DEFINITION  Chloraphenicol resistance gene with dif and 5amyE
ACCESSION   BBa_K316024
VERSION     BBa_K316024.1  
FEATURES             Location/Qualifiers
     misc_feature    1..522
                     /label=5' AmyE homologous sequence
     misc_feature    1..522
                     /label=BBa_K143001
     misc_feature    531..558
                     /label=BBa_K316002
     RBS             567..578
                     /label=rbs
     RBS             567..578
                     /label=BBa_K143021
     misc_feature    585..1244
                     /label=CmR gene
     CDS             585..1244
                     /label=BBa_J31005
     stem_loop       1264..1307
                     /label=stem_loop
     misc_feature    1253..1332
                     /label=BBa_B0010
     terminator      1253..1332
                     /label=BBa_B0010
     stem_loop       1348..1367
                     /label=T7 TE
     misc_feature    1374..1374
                     /label=stop
     polyA_site      1368..1381
                     /label=polya
     misc_feature    1341..1381
                     /label=BBa_B0012
     terminator      1341..1381
                     /label=BBa_B0012
     misc_feature    585..1381
                     /label=BBa_K143064
ORIGIN
        1 atgtttgcaa aacgattcaa aacctcttta ctgccgttat tcgctggatt tttattgctg
       61 tttcatttgg ttctggcagg accggcggct gcgagtgctg aaacggcgaa caaatcgaat
      121 gagcttacag caccgtcgat caaaagcgga accattcttc atgcatggaa ttggtcgttc
      181 aatacgttaa aacacaatat gaaggatatt catgatgcag gatatacagc cattcagaca
      241 tctccgatta accaagtaaa ggaagggaat caaggagata aaagcatgtc gaactggtac
      301 tggctgtatc agccgacatc gtatcaaatt ggcaaccgtt acttaggtac tgaacaagaa
      361 tttaaagaaa tgtgtgcagc cgctgaagaa tatggcataa aggtcattgt tgacgcggtc
      421 atcaatcata ccaccagtga ttatgccgcg atttccaatg aggttaagag tattccaaac
      481 tggacacatg gaaacacaca aattaaaaac tggtctgatc gatactagag atctcctaga
      541 atatatatta tgtaaactta ctagagaaag gtggtgaata ctagatggag aaaaaaatca
      601 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
      661 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
      721 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
      781 tgatgaatgc tcatccggaa tttcgtatgg caatgaaaga cggtgagctg gtgatatggg
      841 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
      901 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
      961 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct
     1021 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact
     1081 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc
     1141 cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta
     1201 atgaattaca acagtactgc gatgagtggc agggcggggc gtaatactag agccaggcat
     1261 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     1321 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     1381 a
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