LOCUS BBa_K316027 3977 bp DNA linear 11-May-2024 DEFINITION B.subtilis transformation vector with LacI ACCESSION BBa_K316027 VERSION BBa_K316027.1 FEATURES Location/Qualifiers misc_feature 1..522 /label=5' AmyE homologous sequence misc_feature 1..522 /label=BBa_K143001 promoter 593..598 /label=Sigma A-35 promoter 616..621 /label=Sigma A -10 promoter 531..627 /label=BBa_K143012 RBS 636..647 /label=rbs RBS 636..647 /label=BBa_K143021 misc_feature 531..647 /label=BBa_K143053 CDS 654..1418 /label=Aad9 Spectinomycin Acetyltransferase misc_feature 1419..1421 /label=stop misc_feature 1422..1424 /label=stop misc_feature 654..656 /label=start CDS 654..1424 /label=BBa_K143031 stem_loop 1444..1487 /label=stem_loop misc_feature 1433..1512 /label=BBa_B0010 terminator 1433..1512 /label=BBa_B0010 stem_loop 1528..1547 /label=T7 TE misc_feature 1554..1554 /label=stop polyA_site 1548..1561 /label=polya misc_feature 1521..1561 /label=BBa_B0012 terminator 1521..1561 /label=BBa_B0012 misc_feature 654..1561 /label=BBa_K143065 promoter 1632..1637 /label=Sigma A-35 promoter 1655..1660 /label=Sigma A -10 promoter 1570..1666 /label=BBa_K143012 RBS 1675..1686 /label=rbs RBS 1675..1686 /label=BBa_K143021 misc_feature 1570..1686 /label=BBa_K143053 CDS 1693..2772 /label=LacI (Lva-, N-terminal deletion) regulatory protein misc_feature 1693..1695 /label=start misc_feature 2773..2775 /label=stop misc_feature 2776..2778 /label=stop CDS 1693..2778 /label=BBa_K143033 stem_loop 2798..2841 /label=stem_loop misc_feature 2787..2866 /label=BBa_B0010 terminator 2787..2866 /label=BBa_B0010 stem_loop 2882..2901 /label=T7 TE misc_feature 2908..2908 /label=stop polyA_site 2902..2915 /label=polya misc_feature 2875..2915 /label=BBa_B0012 terminator 2875..2915 /label=BBa_B0012 misc_feature 1693..2915 /label=BBa_K143062 misc_feature 2924..2951 /label=BBa_K316002 misc_feature 2960..2963 /label=5' product misc_feature 2964..2967 /label=3' product misc_feature 2960..2967 /label=BBa_K316013 misc_feature 2924..2967 /label=BBa_K316014 misc_feature 2976..3980 /label=3' AmyE homologous sequence misc_feature 2976..3977 /label=BBa_K143002 ORIGIN 1 atgtttgcaa aacgattcaa aacctcttta ctgccgttat tcgctggatt tttattgctg 61 tttcatttgg ttctggcagg accggcggct gcgagtgctg aaacggcgaa caaatcgaat 121 gagcttacag caccgtcgat caaaagcgga accattcttc atgcatggaa ttggtcgttc 181 aatacgttaa aacacaatat gaaggatatt catgatgcag gatatacagc cattcagaca 241 tctccgatta accaagtaaa ggaagggaat caaggagata aaagcatgtc gaactggtac 301 tggctgtatc agccgacatc gtatcaaatt ggcaaccgtt acttaggtac tgaacaagaa 361 tttaaagaaa tgtgtgcagc cgctgaagaa tatggcataa aggtcattgt tgacgcggtc 421 atcaatcata ccaccagtga ttatgccgcg atttccaatg aggttaagag tattccaaac 481 tggacacatg gaaacacaca aattaaaaac tggtctgatc gatactagag aattttgtca 541 aaataatttt attgacaacg tcttattaac gttgatataa tttaaatttt atttgacaaa 601 aatgggctcg tgttgtacaa taaatgttac tagagaaagg tggtgaatac tagatgagga 661 ggatatattt gaatacatac gaacaaatta ataaagtgaa aaaaatactt cggaaacatt 721 taaaaaataa ccttattggt acttacatgt ttggatcagg agttgagagt ggactaaaac 781 caaatagtga tcttgacttt ttagtcgtcg tatctgaacc attgacagat caaagtaaag 841 aaatacttat acaaaaaatt agacctattt caaaaaaaat aggagataaa agcaacttac 901 gatatattga attaacaatt attattcagc aagaaatggt accgtggaat catcctccca 961 aacaagaatt tatttatgga gaatggttac aagagcttta tgaacaagga tacattcctc 1021 agaaggaatt aaattcagat ttaaccataa tgctttacca agcaaaacga aaaaataaaa 1081 gaatatacgg aaattatgac ttagaggaat tactacctga tattccattt tctgatgtga 1141 gaagagccat tatggattcg tcagaggaat taatagataa ttatcaggat gatgaaacca 1201 actctatatt aactttatgc cgtatgattt taactatgga cacgggtaaa atcataccaa 1261 aagatattgc gggaaatgca gtggctgaat cttctccatt agaacatagg gagagaattt 1321 tgttagcagt tcgtagttat cttggagaga atattgaatg gactaatgaa aatgtaaatt 1381 taactataaa ctatttaaat aacagattaa aaaaattata ataatactag agccaggcat 1441 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 1501 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 1561 atactagaga attttgtcaa aataatttta ttgacaacgt cttattaacg ttgatataat 1621 ttaaatttta tttgacaaaa atgggctcgt gttgtacaat aaatgttact agagaaaggt 1681 ggtgaatact agatgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct 1741 tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa 1801 aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg 1861 gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg 1921 tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg 1981 tcgatggtag aacgaagcgg cgtcgaagcc tgtaaaacgg cggtgcacaa tcttctcgcg 2041 caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg 2101 gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc 2161 aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca 2221 ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg 2281 cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg 2341 gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc 2401 atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc 2461 attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata cgacgatacc 2521 gaagacagct catgttatat cccgccgtca accaccatca aacaggattt tcgcctgctg 2581 gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat 2641 cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc 2701 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg 2761 gaaagcgggc agtaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 2821 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 2881 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagatctcct agaatatata 2941 ttatgtaaac ttactagagg aaatttctac tagagatccg tttaggctgg gcggtgatag 3001 cttctcgttc aggcagtacg cctcttttct tttccagacc tgagggaggc ggaaatggtg 3061 tgaggttccc ggggaaaagc caaataggcg atcgcgggag tgctttattt gaagatcagg 3121 ctatcactgc ggtcaataga tttcacaatg tgatggctgg acagcctgag gaactctcga 3181 acccgaatgg aaacaaccag atatttatga atcagcgcgg ctcacatggc gttgtgctgg 3241 caaatgcagg ttcatcctct gtctctatca atacggcaac aaaattgcct gatggcaggt 3301 atgacaataa agctggagcg ggttcatttc aagtgaacga tggtaaactg acaggcacga 3361 tcaatgccag gtctgtagct gtgctttatc ctgatgatat tgcaaaagcg cctcatgttt 3421 tccttgagaa ttacaaaaca ggtgtaacac attctttcaa tgatcaactg acgattacct 3481 tgcgtgcaga tgcgaataca acaaaagccg tttatcaaat caataatgga ccagagacgg 3541 cgtttaagga tggagatcaa ttcacaatcg gaaaaggaga tccatttggc aaaacataca 3601 ccatcatgtt aaaaggaacg aacagtgatg gtgtaacgag gaccgagaaa tacagttttg 3661 ttaaaagaga tccagcgtcg gccaaaacca tcggctatca aaatccgaat cattggagcc 3721 aggtaaatgc ttatatctat aaacatgatg ggagccgagt aattgaattg accggatctt 3781 ggcctggaaa accaatgact aaaaatgcag acggaattta cacgctgacg ctgcctgcgg 3841 acacggatac aaccaacgca aaagtgattt ttaataatgg cagcgcccaa gtgcccggtc 3901 agaatcagcc tggctttgat tacgtgctaa atggtttata taatgactcg ggcttaagcg 3961 gttctcttcc ccattga //