LOCUS       BBa_K316044             1182 bp    DNA     linear       19-May-2024
DEFINITION  LytC - Helical Linker - TEV Cleavage Site - AIP - His Tag
ACCESSION   BBa_K316044
VERSION     BBa_K316044.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=sRBS
     misc_feature    22..24
                     /label=start
     CDS             22..976
                     /label=LytC Cell Wall Binding Domain
     CDS             977..1053
                     /label=Helical Linker
     CDS             1054..1107
                     /label=TEV Cleavage Site
     CDS             1108..1158
                     /label=Auto Inducing Peptide
     misc_feature    1159..1176
                     /label=His Tag
     misc_feature    1177..1179
                     /label=Stop Codon
     misc_feature    1180..1182
                     /label=Stop Codon
ORIGIN
        1 tcttcagaaa ggagttacta gatgcgttct tatataaaag tcctaacaat gtgttttctg
       61 gggctcatac tttttgtgcc aacagctttg gccgataact cagtgaaaag agttggggga
      121 agcaatagat acggcactgc tgtacaaata tcaaagcaaa tgtattcaac agcaagtaca
      181 gctgtaattg ttggtgggag ttcctatgca gatgctattt cagcagcacc tcttgcttac
      241 cagaagaatg cgccattgct ttacactaat tctgataagc tttcatatga aacgaaaaca
      301 agattgaaag agatgcagac taaaaatgta attattgtag gcggaacacc tgctgtttct
      361 tctaacactg ctaaccagat taaaagcttg gggataagta ttaaacgaat tgcaggaagc
      421 aaccgttatg atacggctgc acgggtggca aaagcgatgg gtgcgacttc aaaagctgtt
      481 attttgaacg gcttcttata tgcagacgct ccggccgtca tcccttatgc agcgaaaaac
      541 gggtatccaa ttctttttac aaataaaaca tctataaata gtgcgactac gtctgtgata
      601 aaagataagg gaatttcgag taccgttgtt gtaggaggca ctggaagtat cagcaatacg
      661 gtatacaaca agttaccttc tcctacaaga attagcggtt caaacagata tgagcttgct
      721 gcaaatatcg tacaaaaact taatttatca acaagcaccg tatatgtaag caatggattc
      781 agctaccctg actctattgc aggagctaca ctggcagcta agaagaagca atctcttatt
      841 cttacaaatg gtgaaaattt atctacagga gcccgtaaaa ttattggaag taaaaacatg
      901 tcaaacttta tgattatcgg aaacactcct gccgtaagca caaaggttgc taatcagcta
      961 aagaatccag ttgtaagcag aggctcacgc gcactggcag aagccgcagc taaggaagct
     1021 gctgcaaaag aggcggcggc gaaagccgcg gccggaggtg gaggtgaaaa tctttatttt
     1081 caaggaggca aactgggagg cggcggagaa atgcgtctgt caaaattctt tcgcgatttt
     1141 attcttcaaa gaaaaaagca tcaccatcat caccattaat aa
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