LOCUS BBa_K317040 3285 bp DNA linear 10-Jun-2024 DEFINITION Pconst.(L)-RBS-LuxI-Double term-Plac-Lysis ACCESSION BBa_K317040 VERSION BBa_K317040.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23109 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 62..1147 /label=BBa_I732100 stem_loop 1167..1210 /label=stem_loop misc_feature 1156..1235 /label=BBa_B0010 terminator 1156..1235 /label=BBa_B0010 stem_loop 1251..1270 /label=T7 TE misc_feature 1277..1277 /label=stop polyA_site 1271..1284 /label=polya misc_feature 1244..1284 /label=BBa_B0012 terminator 1244..1284 /label=BBa_B0012 misc_feature 44..1284 /label=BBa_I732820 CDS 1293..1380 /label=end of LacI coding region (inactive) misc_feature 1293..1492 /label=BBa_R0010 misc_feature 1381..1418 /label=CAP binding site promoter 1429..1434 /label=-35 promoter 1453..1458 /label=-10 misc_feature 1458..1492 /label=LacI binding site misc_feature 1465..1465 /label=start promoter 1293..1492 /label=BBa_R0010 misc_feature 1..1492 /label=BBa_K317042 CDS 1517..2173 /label=T4 holin CDS 1501..2176 /label=BBa_K112805 CDS 2201..2695 /label=T4 Endolysin CDS 2185..2698 /label=BBa_K112806 stem_loop 2718..2761 /label=stem_loop misc_feature 2707..2786 /label=BBa_B0010 terminator 2707..2786 /label=BBa_B0010 stem_loop 2802..2821 /label=T7 TE misc_feature 2828..2828 /label=stop polyA_site 2822..2835 /label=polya misc_feature 2795..2835 /label=BBa_B0012 terminator 2795..2835 /label=BBa_B0012 promoter 2844..2878 /label=BBa_J23116 CDS 2901..3194 /label=T4 antiholin CDS 2887..3197 /label=BBa_K112807 stem_loop 3217..3260 /label=stem_loop misc_feature 3206..3285 /label=BBa_B0010 terminator 3206..3285 /label=BBa_B0010 misc_feature 1501..3285 /label=BBa_K112808 ORIGIN 1 tttacagcta gctcagtcct agggactgtg ctagctacta gagaaagagg agaaatacta 61 gatgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt 121 ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc 181 ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca 241 gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt 301 cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga 361 acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag 421 tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg 481 cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat 541 tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca 601 gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg 661 ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg 721 gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac 781 tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc 841 cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc 901 atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag 961 cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc 1021 cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg 1081 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca 1141 gtaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 1201 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt 1261 cgggtgggcc tttctgcgtt tatatactag agcaatacgc aaaccgcctc tccccgcgcg 1321 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga 1381 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat 1441 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca catactagag 1501 cttaaaagga gggtctatgg cagcacctag aatatcattt tcgccctctg atattctatt 1561 tggtgttctc gatcgcttgt tcaaagataa cgctaccggg aaggttcttg cttcccgggt 1621 agctgtcgta attcttttgt ttataatggc gattgtttgg tataggggag atagtttctt 1681 tgagtactat aagcaatcaa agtatgaaac atacagtgaa attattgaaa aggaaagaac 1741 tgcacgcttt gaatctgtcg ccctggaaca actccagata gttcatatat catctgaggc 1801 agactttagt gcggtgtatt ctttccgccc taaaaactta aactattttg ttgatattat 1861 agcatacgaa ggaaaattac cttcaacaat aagtgaaaaa tcacttggag gatatcctgt 1921 tgataaaact atggatgaat atacagttca tttaaatgga cgtcattatt attccaactc 1981 aaaatttgct tttttaccaa ctaaaaagcc tactcccgaa ataaactaca tgtacagttg 2041 tccatatttt aatttggata atatctatgc tggaacgata accatgtact ggtatagaaa 2101 tgatcatata agtaatgacc gccttgaatc aatatgtgct caggcggcca gaatattagg 2161 aagggctaaa taattatact agagatactt aggaggtatt atgaatatat ttgaaatgtt 2221 acgtatagat gaaggtctta gacttaaaat ctataaagac acagaaggct attacactat 2281 tggcatcggt catttgctta caaaaagtcc atcacttaat gctgctaaat ctgaattaga 2341 taaagctatt gggcgtaatt gcaatggtgt aattacaaaa gatgaggctg aaaaactctt 2401 taatcaggat gttgatgctg ctgttcgcgg aatcctgaga aatgctaaat taaaaccggt 2461 ttatgattct cttgatgcgg ttcgtcgctg tgcattgatt aatatggttt tccaaatggg 2521 agaaaccggt gtggcaggat ttactaactc tttacgtatg cttcaacaaa aacgctggga 2581 tgaagcagca gttaacttag ctaaaagtag atggtataat caaacaccta atcgcgcaaa 2641 acgagtcatt acaacgttta gaactggcac ttgggacgcg tataaaaatc tataaagcta 2701 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 2761 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 2821 ctttctgcgt ttatatacta gagttgacag ctagctcagt cctagggact atgctagcta 2881 ctagaggata ggaggccttt atggccttaa aagcaacagc actttttgcc atgctaggat 2941 tgtcatttgt tttatctcca tcgattgaag cgaatgtcga tcctcatttt gataaattta 3001 tggaatctgg tattaggcac gtttatatgc tttttgaaaa taaaagcgta gaatcgtctg 3061 aacaattcta tagttttatg agaacgacct ataaaaatga cccgtgctct tctgattttg 3121 aatgtataga gcgaggcgcg gagatggcac aatcatacgc tagaattatg aacattaaat 3181 tggagactga atgaaattac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 3241 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctc //