LOCUS BBa_K318000 4395 bp DNA linear 01-Jun-2024 DEFINITION pBad + Cre + pLac + Hin + pCons + lacI ACCESSION BBa_K318000 VERSION BBa_K318000.1 FEATURES Location/Qualifiers misc_feature 1..39 /label=B1006 stem_loop 10..31 /label=modified thr terminator polyA_site 32..39 /label=PolyA polyA_site 1..9 /label=PolyA terminator 1..39 /label=BBa_B1006 promoter 1127..1257 /label=Pbad promoter 1090..1161 /label=PC misc_feature 48..1126 /label=AraC promoter 48..1257 /label=BBa_I0500 RBS 1266..1280 /label=RBS-1\Strong RBS 1273..1277 /label=RBS misc_feature 1266..1280 /label=BBa_B0030 RBS 1266..1280 /label=BBa_B0030 CDS 1287..2323 /label=BBa_J61047 misc_feature 48..2323 /label=BBa_I718008 misc_feature 2332..2370 /label=B1006 stem_loop 2341..2362 /label=modified thr terminator polyA_site 2363..2370 /label=PolyA polyA_site 2332..2340 /label=PolyA terminator 2332..2370 /label=BBa_B1006 misc_feature 2379..2417 /label=B1006 stem_loop 2388..2409 /label=modified thr terminator polyA_site 2410..2417 /label=PolyA polyA_site 2379..2387 /label=PolyA terminator 2379..2417 /label=BBa_B1006 misc_feature 2428..2444 /label=lac O1 promoter 2445..2450 /label=-35 misc_feature 2451..2467 /label=lac O1 promoter 2468..2473 /label=-10 misc_feature 2426..2479 /label=BBa_R0011 promoter 2426..2479 /label=BBa_R0011 misc_feature 2493..2496 /label=conserved RBS 2489..2500 /label=BBa_B0034 misc_feature 2426..2500 /label=BBa_K200021 misc_feature 2576..2581 /label=Former SpeI site misc_feature 2578..2578 /label=Mutation to delete the SpeI site CDS 2507..3079 /label=Hin sequence primer_bind 2507..2509 /label=Fwd primer J31001 primer_bind 3057..3076 /label=Rev primer J31001 CDS 2507..3079 /label=BBa_J31000 misc_feature 3088..3126 /label=B1006 stem_loop 3097..3118 /label=modified thr terminator polyA_site 3119..3126 /label=PolyA polyA_site 3088..3096 /label=PolyA terminator 3088..3126 /label=BBa_B1006 promoter 3135..3169 /label=BBa_J23100 misc_feature 3182..3185 /label=conserved RBS 3178..3189 /label=BBa_B0034 misc_feature 4285..4323 /label=LVA CDS 3196..4323 /label=lacI-LVA misc_feature 3196..4323 /label=BBa_C0012 misc_feature 4324..4348 /label=Help:Barcodes CDS 3196..4323 /label=BBa_C0012 misc_feature 3178..4348 /label=BBa_S03520 misc_feature 4357..4395 /label=B1006 stem_loop 4366..4387 /label=modified thr terminator polyA_site 4388..4395 /label=PolyA polyA_site 4357..4365 /label=PolyA terminator 4357..4395 /label=BBa_B1006 ORIGIN 1 aaaaaaaaac cccgcccctg acagggcggg gttttttttt actagagtta tgacaacttg 61 acggctacat cattcacttt ttcttcacaa ccggcacgga actcgctcgg gctggccccg 121 gtgcattttt taaatacccg cgagaaatag agttgatcgt caaaaccaac attgcgaccg 181 acggtggcga taggcatccg ggtggtgctc aaaagcagct tcgcctggct gatacgttgg 241 tcctcgcgcc agcttaagac gctaatccct aactgctggc ggaaaagatg tgacagacgc 301 gacggcgaca agcaaacatg ctgtgcgacg ctggcgatat caaaattgct gtctgccagg 361 tgatcgctga tgtactgaca agcctcgcgt acccgattat ccatcggtgg atggagcgac 421 tcgttaatcg cttccatgcg ccgcagtaac aattgctcaa gcagatttat cgccagcagc 481 tccgaatagc gcccttcccc ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa 541 tgcggctggt gcgcttcatc cgggcgaaag aaccccgtat tggcaaatat tgacggccag 601 ttaagccatt catgccagta ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg 661 cgagcctccg gatgacgacc gtagtgatga atctctcctg gcgggaacag caaaatatca 721 cccggtcggc aaacaaattc tcgtccctga tttttcacca ccccctgacc gcgaatggtg 781 agattgagaa tataaccttt cattcccagc ggtcggtcga taaaaaaatc gagataaccg 841 ttggcctcaa tcggcgttaa acccgccacc agatgggcat taaacgagta tcccggcagc 901 aggggatcat tttgcgcttc agccatactt ttcatactcc cgccattcag agaagaaacc 961 aattgtccat attgcatcag acattgccgt cactgcgtct tttactggct cttctcgcta 1021 accaaaccgg taaccccgct tattaaaagc attctgtaac aaagcgggac caaagccatg 1081 acaaaaacgc gtaacaaaag tgtctataat cacggcagaa aagtccacat tgattatttg 1141 cacggcgtca cactttgcta tgccatagca tttttatcca taagattagc ggatcctacc 1201 tgacgctttt tatcgcaact ctctactgtt tctccatacc cgtttttttg ggctagctac 1261 tagagattaa agaggagaaa tactagatgt ccaatttact gaccgtacac caaaatttgc 1321 ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg gacatgttca 1381 gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt tgccggtcgt 1441 gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct gaagatgttc 1501 gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaactatc cagcaacatt 1561 tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt gacagcaatg 1621 ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt gaacgtgcaa 1681 aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc atggaaaata 1741 gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat aacaccctgt 1801 tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact gacggtggga 1861 gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt gtagagaagg 1921 cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct ggtgtagctg 1981 atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc gcgccatctg 2041 ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact catcgattga 2101 tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga cacagtgccc 2161 gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag atcatgcaag 2221 ctggtggctg gaccaatgta aatattgtca tgaactatat ccgtaacctg gatagtgaaa 2281 caggggcaat ggtgcgcctg ctggaagatg gcgattaaga atttactaga gaaaaaaaaa 2341 ccccgcccct gacagggcgg ggtttttttt tactagagaa aaaaaaaccc cgcccctgac 2401 agggcggggt ttttttttac tagagaattg tgagcggata acaattgaca ttgtgagcgg 2461 ataacaagat actgagcaca tactagagaa agaggagaaa tactagatgg ctactattgg 2521 gtatattcgg gtgtcaacaa ttgaccaaaa tatcgattta cagcgtaatg cgcttaccag 2581 tgcaaattgt gaccgcattt ttgaagaccg tatcagtggc aagattgcaa accgccccgg 2641 cctgaaacgg gcgttaaagt atgtaaataa aggcgatact cttgtcgtct ggaaattaga 2701 cagactgggc cgtagcgtga aaaatctggt ggcgttaata tcagaattac atgaacgtgg 2761 agctcacttc cattctttaa ccgatagtat tgataccagt agcgcgatgg ggcgattctt 2821 ttttcatgta atgtcagcac tggccgagat ggagcgagaa ttaatcgtcg agcgaaccct 2881 tgccggactg gctgccgcca gagcgcaagg acgactggga gggcgccctc gggcgatcaa 2941 caaacatgaa caggaacaga ttagtcggct attagagaaa ggccatcctc ggcagcaatt 3001 agctattatt tttggtattg gcgtatccac cttatacaga tactttccgg caagcagtat 3061 aaaaaaacga atgaattaat actagagaaa aaaaaacccc gcccctgaca gggcggggtt 3121 tttttttact agagttgacg gctagctcag tcctaggtac agtgctagct actagagaaa 3181 gaggagaaat actagatggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat 3241 gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg 3301 aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg 3361 gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc 3421 ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc 3481 agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac 3541 aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat 3601 gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac 3661 cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag 3721 catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc 3781 tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg 3841 atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg 3901 ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc 3961 gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga 4021 tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat 4081 tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg 4141 gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc 4201 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag 4261 gtttcccgac tggaaagcgg gcaggctgca aacgacgaaa actacgcttt agtagcttaa 4321 taactctgat agtgctagtg tagatctcta ctagagaaaa aaaaaccccg cccctgacag 4381 ggcggggttt ttttt //