LOCUS       BBa_K318021              654 bp    DNA     linear       17-May-2024
DEFINITION  pLac + RBS + Hin
ACCESSION   BBa_K318021
VERSION     BBa_K318021.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    151..156
                     /label=Former SpeI site
     misc_feature    153..153
                     /label=Mutation to delete the SpeI site
     CDS             82..654
                     /label=Hin sequence
     primer_bind     82..84
                     /label=Fwd primer J31001
     primer_bind     632..651
                     /label=Rev primer J31001
     CDS             82..654
                     /label=BBa_J31000
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatggctact attgggtata ttcgggtgtc aacaattgac
      121 caaaatatcg atttacagcg taatgcgctt accagtgcaa attgtgaccg catttttgaa
      181 gaccgtatca gtggcaagat tgcaaaccgc cccggcctga aacgggcgtt aaagtatgta
      241 aataaaggcg atactcttgt cgtctggaaa ttagacagac tgggccgtag cgtgaaaaat
      301 ctggtggcgt taatatcaga attacatgaa cgtggagctc acttccattc tttaaccgat
      361 agtattgata ccagtagcgc gatggggcga ttcttttttc atgtaatgtc agcactggcc
      421 gagatggagc gagaattaat cgtcgagcga acccttgccg gactggctgc cgccagagcg
      481 caaggacgac tgggagggcg ccctcgggcg atcaacaaac atgaacagga acagattagt
      541 cggctattag agaaaggcca tcctcggcag caattagcta ttatttttgg tattggcgta
      601 tccaccttat acagatactt tccggcaagc agtataaaaa aacgaatgaa ttaa
//