LOCUS       BBa_K318026              954 bp    DNA     linear       17-May-2024
DEFINITION  pCons + attB + RBS + GFP + T
ACCESSION   BBa_K318026
VERSION     BBa_K318026.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    44..70
                     /label=BBa_I11032
     RBS             79..91
                     /label=RBS-3\Weak
     RBS             85..88
                     /label=RBS
     misc_feature    79..91
                     /label=BBa_B0032
     RBS             79..91
                     /label=BBa_B0032
     CDS             98..817
                     /label=GFP protein
     CDS             98..817
                     /label=BBa_E0040
     stem_loop       837..880
                     /label=stem_loop
     misc_feature    826..905
                     /label=BBa_B0010
     terminator      826..905
                     /label=BBa_B0010
     stem_loop       921..940
                     /label=T7 TE
     misc_feature    947..947
                     /label=stop
     polyA_site      941..954
                     /label=polya
     misc_feature    914..954
                     /label=BBa_B0012
     terminator      914..954
                     /label=BBa_B0012
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagacgacct tcgcattacg
       61 aatgcgctgc tactagagtc acacaggaaa gtactagatg cgtaaaggag aagaactttt
      121 cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc
      181 tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg
      241 cactactgga aaactacctg ttccatggcc aacacttgtc actactttcg gttatggtgt
      301 tcaatgcttt gcgagatacc cagatcatat gaaacagcat gactttttca agagtgccat
      361 gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac
      421 acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat
      481 tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca
      541 caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag
      601 acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat
      661 tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc
      721 gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg
      781 gattacacat ggcatggatg aactatacaa ataataatac tagagccagg catcaaataa
      841 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
      901 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//