BBa_K318030
1
"Lock"
lox66 + rpCons + hixC + lox71 + SapI + T + T + Hin enhancer + HindIII + hixC
2010-07-12T11:00:00Z
2015-05-08T01:11:58Z
From Synthesis
This composite part is a prefix ???lock??? cassette requiring consecutive recombination by Hin and Cre, in that order, to express a gene downstream of the hixC site. The SapI restriction site can be used to insert DNA with ATC sticky ends in order to modify the length of recombination. After Hin recombination, the part becomes: lox66 + rpCons + hixC + HindIII + rHin enhancer + T + T + SapI + lox71 + hixC. When Cre is used after Hin, the part becomes: mloxP + SapI + T + T + Hin enhancer + HindIII + hixC + pCons + loxP + hixC.
false
false
_438_
0
6061
9
Not in stock
true
Note that the constitutive promoter is oriented to express whatever gene is downstream of the final hixC site.
false
Justin Vrana, Nate Pantalone
component2084236
1
BBa_I718016
component2084245
1
BBa_B1006
component2084250
1
BBa_B1006
component2084252
1
BBa_J44000
component2084237
1
BBa_K318050
component2084240
1
BBa_K318051
component2084238
1
BBa_J44000
component2084251
1
BBa_K318052
component2084239
1
BBa_I718017
annotation2084236
1
BBa_I718016
range2084236
1
1
34
annotation2084251
1
BBa_K318052
range2084251
1
275
357
annotation2084252
1
BBa_J44000
range2084252
1
366
391
annotation2084237
1
BBa_K318050
range2084237
1
43
77
annotation2084238
1
BBa_J44000
range2084238
1
86
111
annotation2084245
1
BBa_B1006
range2084245
1
181
219
annotation2084250
1
BBa_B1006
range2084250
1
228
266
annotation2084239
1
BBa_I718017
range2084239
1
120
153
annotation2084240
1
BBa_K318051
range2084240
1
162
172
BBa_K318052
1
BBa_K318052
RE+HindIII
2010-08-26T11:00:00Z
2015-05-08T01:11:58Z
42
BBa_J3101 with a HindIII site added to the end.
false
false
_438_
0
4845
9
Not in stock
false
None
false
Nathaniel Pantalone
BBa_J44000
1
hixC
hixC binding site for Salmonella typhimurium Hin recombinase
2006-06-05T11:00:00Z
2015-08-31T04:08:48Z
Nanassy and Hughes. 1998. In Vivo Identification of Intermediate Stages of the DNA Inversion Reaction Catlyzed by the Salmonella Hin Recombinase
[http://www.genetics.org/cgi/content/abstract/149/4/1649]
A 26 bp sequence of DNA composed of 12 bp inverted repeats and a 2 bp core that operates in Salmonella paired with a hixR binding site to recombine DNA. A second hix site is required for recombination to occur. The two sites bind Hin recombinase in the formation of an invertasome.
false
true
_71_
0
606
61
In stock
true
Standard BioBrick prefix and suffix were added to the 26 bp sequence.
true
Missouri Western and Davidson Groups, Todd Eckdahl
BBa_B1006
1
BBa_B1006
Terminator (artificial, large, %T~>90)
2006-08-30T11:00:00Z
2015-08-31T04:07:21Z
modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
Released HQ 2013
Artificial terminator, estimated %T~>90%
*8bp stem, 6nt loop
*Bidirectional, estimated reverse %T~>90%
false
true
_41_
0
745
41
In stock
false
Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
true
Haiyao Huang
annotation1898430
1
PolyA
range1898430
1
32
39
annotation1898431
1
PolyA
range1898431
1
1
9
annotation1898428
1
B1006
range1898428
1
1
39
annotation1898429
1
modified thr terminator
range1898429
1
10
31
BBa_I718017
1
lox71
lox71
2007-10-25T11:00:00Z
2015-08-31T04:07:52Z
This part was generated in the form of a forward & a reverse primer. After annealing these primers EcoRI & PstI compatible cohesive ends at the 5' & 3' ends of the dsDNA were generated.
Next, the dsDNA was subcloned in a pSB1A2 open plasmid (digested with EcoRI & PstI)
You can follow the construction process by following the links available in the Paris iGEM 2007 wiki:
http://parts.mit.edu/igem07/index.php/Paris
"freezer" section
plasmids table. A links sends you to the corresponding notebook date when the ligation reaction was performed
Released HQ 2013
Lox71 is a site specific recombination cassette. It belongs to the loxP family frequently used in genetics, particularly in mouse genetics.
lox site recombination is catalysed by a Site specific recombinase, Cre.
lox sequences are composed of an 8 bp Core sequence surrounded by two Arms.
The particularity of lox66 is that it has an altered sequence at the end of it's left arm compared to loxP. This sequence variation reduces affinity of the Cre recombinase for the arm.
As a consequence, after a recombination between a lox71 and a lox66 (altered right arm sequence), one of the two resulting generated lox sites has very low recombination potential as it inherited both mutated arms. Use of lox71 & lox66 sites is potentially interesting when the recombination reaction must be "irreversible".
false
false
_141_
0
1568
9
In stock
false
No modifaication was made on lox71 sequence
true
Eimad Shotar
BBa_K318050
1
BBa_K318050
rPcons (J23100)
2010-08-26T11:00:00Z
2015-05-08T01:11:58Z
Registry
The reverse compliment of BBa_J23100
false
false
_438_
0
4845
9
Not in stock
false
This part expresses very strongly. Pick smaller colonies when screening.
false
Nathaniel Pantalone
BBa_K318051
1
BBa_K318051
SapI site
2010-08-26T11:00:00Z
2015-05-08T01:11:58Z
Life
SapI site for junk DNA addition by multimerization of oligonucleotides.
false
false
_438_
0
4845
9
Not in stock
false
The three bases that make up the sticky ends after digestion are ATC
false
Nathaniel Pantalone
BBa_I718016
1
lox66
lox66
2007-10-25T11:00:00Z
2015-08-31T04:07:52Z
This part was generated in the form of a forward & a reverse primer. After annealing these primers EcoRI & PstI compatible cohesive ends at the 5' & 3' ends of the dsDNA were generated.
Next, the dsDNA was subcloned in a pSB1A2 open plasmid (digested with EcoRI & PstI)
You can follow the construction process by following the links available in the Paris iGEM 2007 wiki:
http://parts.mit.edu/igem07/index.php/Paris
"freezer" section
plasmids table. A links sends you to the corresponding notebook date when the ligation reaction was performed
lox66 is a site specific recombination cassette. It belongs to the loxP family frequently used in genetics, particularily in mouse genetics.
lox site recombination is catalysed by a Site specific recombinase, Cre.
lox sequences are composed of an 8 bp Core sequence surrounded by two Arms.
The particularity of lox66 is that it has an altered sequence at the end of it's left arm compared to loxP. This sequence variation reduces affinity of the Cre recombinase for the arm.
As a consequence, after a recombination between a lox66 and a lox71 (altered right arm sequence), one of the two resulting generated lox sites has very low recombination potential as it inherited both mutated arms. Use of lox66 & lox71 sites is potentially interresting when the recombination reaction must be "irreversible".
false
false
_141_
0
1568
9
In stock
false
No modidification was made on the lox66 sequence
true
Eimad Shotar
BBa_K318052_sequence
1
ttcgggtgtcaacaattgaccaaaatattgatttacagcgtaatgcgctttctagtgcaaattgtgaccgcattttgaagctt
BBa_I718017_sequence
1
taccgttcgtatacgatacattatacgaagttat
BBa_J44000_sequence
1
ttatcaaaaaccatggtttttgataa
BBa_K318030_sequence
1
ataacttggtatagcatacattatacgaacggtatactagaggctagcactgtacctaggactgagctagccgtcaatactagagttatcaaaaaccatggtttttgataatactagagtaccgttcgtatacgatacattatacgaagttattactagaggctcttcaatctactagagaaaaaaaaaccccgcccctgacagggcggggtttttttttactagagaaaaaaaaaccccgcccctgacagggcggggtttttttttactagagttcgggtgtcaacaattgaccaaaatattgatttacagcgtaatgcgctttctagtgcaaattgtgaccgcattttgaagctttactagagttatcaaaaaccatggtttttgataa
BBa_B1006_sequence
1
aaaaaaaaaccccgcccctgacagggcggggtttttttt
BBa_I718016_sequence
1
ataacttggtatagcatacattatacgaacggta
BBa_K318050_sequence
1
gctagcactgtacctaggactgagctagccgtcaa
BBa_K318051_sequence
1
gctcttcaatc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z