BBa_K318030 1 "Lock" lox66 + rpCons + hixC + lox71 + SapI + T + T + Hin enhancer + HindIII + hixC 2010-07-12T11:00:00Z 2015-05-08T01:11:58Z From Synthesis This composite part is a prefix ???lock??? cassette requiring consecutive recombination by Hin and Cre, in that order, to express a gene downstream of the hixC site. The SapI restriction site can be used to insert DNA with ATC sticky ends in order to modify the length of recombination. After Hin recombination, the part becomes: lox66 + rpCons + hixC + HindIII + rHin enhancer + T + T + SapI + lox71 + hixC. When Cre is used after Hin, the part becomes: mloxP + SapI + T + T + Hin enhancer + HindIII + hixC + pCons + loxP + hixC. false false _438_ 0 6061 9 Not in stock true Note that the constitutive promoter is oriented to express whatever gene is downstream of the final hixC site. false Justin Vrana, Nate Pantalone component2084236 1 BBa_I718016 component2084245 1 BBa_B1006 component2084250 1 BBa_B1006 component2084252 1 BBa_J44000 component2084237 1 BBa_K318050 component2084240 1 BBa_K318051 component2084238 1 BBa_J44000 component2084251 1 BBa_K318052 component2084239 1 BBa_I718017 annotation2084236 1 BBa_I718016 range2084236 1 1 34 annotation2084251 1 BBa_K318052 range2084251 1 275 357 annotation2084252 1 BBa_J44000 range2084252 1 366 391 annotation2084237 1 BBa_K318050 range2084237 1 43 77 annotation2084238 1 BBa_J44000 range2084238 1 86 111 annotation2084245 1 BBa_B1006 range2084245 1 181 219 annotation2084250 1 BBa_B1006 range2084250 1 228 266 annotation2084239 1 BBa_I718017 range2084239 1 120 153 annotation2084240 1 BBa_K318051 range2084240 1 162 172 BBa_K318052 1 BBa_K318052 RE+HindIII 2010-08-26T11:00:00Z 2015-05-08T01:11:58Z 42 BBa_J3101 with a HindIII site added to the end. false false _438_ 0 4845 9 Not in stock false None false Nathaniel Pantalone BBa_J44000 1 hixC hixC binding site for Salmonella typhimurium Hin recombinase 2006-06-05T11:00:00Z 2015-08-31T04:08:48Z Nanassy and Hughes. 1998. In Vivo Identification of Intermediate Stages of the DNA Inversion Reaction Catlyzed by the Salmonella Hin Recombinase [http://www.genetics.org/cgi/content/abstract/149/4/1649] A 26 bp sequence of DNA composed of 12 bp inverted repeats and a 2 bp core that operates in Salmonella paired with a hixR binding site to recombine DNA. A second hix site is required for recombination to occur. The two sites bind Hin recombinase in the formation of an invertasome. false true _71_ 0 606 61 In stock true Standard BioBrick prefix and suffix were added to the 26 bp sequence. true Missouri Western and Davidson Groups, Todd Eckdahl BBa_B1006 1 BBa_B1006 Terminator (artificial, large, %T~>90) 2006-08-30T11:00:00Z 2015-08-31T04:07:21Z modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs Released HQ 2013 Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90% false true _41_ 0 745 41 In stock false Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues. true Haiyao Huang annotation1898430 1 PolyA range1898430 1 32 39 annotation1898431 1 PolyA range1898431 1 1 9 annotation1898428 1 B1006 range1898428 1 1 39 annotation1898429 1 modified thr terminator range1898429 1 10 31 BBa_I718017 1 lox71 lox71 2007-10-25T11:00:00Z 2015-08-31T04:07:52Z This part was generated in the form of a forward & a reverse primer. After annealing these primers EcoRI & PstI compatible cohesive ends at the 5' & 3' ends of the dsDNA were generated. Next, the dsDNA was subcloned in a pSB1A2 open plasmid (digested with EcoRI & PstI) You can follow the construction process by following the links available in the Paris iGEM 2007 wiki: http://parts.mit.edu/igem07/index.php/Paris "freezer" section plasmids table. A links sends you to the corresponding notebook date when the ligation reaction was performed Released HQ 2013 Lox71 is a site specific recombination cassette. It belongs to the loxP family frequently used in genetics, particularly in mouse genetics. lox site recombination is catalysed by a Site specific recombinase, Cre. lox sequences are composed of an 8 bp Core sequence surrounded by two Arms. The particularity of lox66 is that it has an altered sequence at the end of it's left arm compared to loxP. This sequence variation reduces affinity of the Cre recombinase for the arm. As a consequence, after a recombination between a lox71 and a lox66 (altered right arm sequence), one of the two resulting generated lox sites has very low recombination potential as it inherited both mutated arms. Use of lox71 & lox66 sites is potentially interesting when the recombination reaction must be "irreversible". false false _141_ 0 1568 9 In stock false No modifaication was made on lox71 sequence true Eimad Shotar BBa_K318050 1 BBa_K318050 rPcons (J23100) 2010-08-26T11:00:00Z 2015-05-08T01:11:58Z Registry The reverse compliment of BBa_J23100 false false _438_ 0 4845 9 Not in stock false This part expresses very strongly. Pick smaller colonies when screening. false Nathaniel Pantalone BBa_K318051 1 BBa_K318051 SapI site 2010-08-26T11:00:00Z 2015-05-08T01:11:58Z Life SapI site for junk DNA addition by multimerization of oligonucleotides. false false _438_ 0 4845 9 Not in stock false The three bases that make up the sticky ends after digestion are ATC false Nathaniel Pantalone BBa_I718016 1 lox66 lox66 2007-10-25T11:00:00Z 2015-08-31T04:07:52Z This part was generated in the form of a forward & a reverse primer. After annealing these primers EcoRI & PstI compatible cohesive ends at the 5' & 3' ends of the dsDNA were generated. Next, the dsDNA was subcloned in a pSB1A2 open plasmid (digested with EcoRI & PstI) You can follow the construction process by following the links available in the Paris iGEM 2007 wiki: http://parts.mit.edu/igem07/index.php/Paris "freezer" section plasmids table. A links sends you to the corresponding notebook date when the ligation reaction was performed lox66 is a site specific recombination cassette. It belongs to the loxP family frequently used in genetics, particularily in mouse genetics. lox site recombination is catalysed by a Site specific recombinase, Cre. lox sequences are composed of an 8 bp Core sequence surrounded by two Arms. The particularity of lox66 is that it has an altered sequence at the end of it's left arm compared to loxP. This sequence variation reduces affinity of the Cre recombinase for the arm. As a consequence, after a recombination between a lox66 and a lox71 (altered right arm sequence), one of the two resulting generated lox sites has very low recombination potential as it inherited both mutated arms. Use of lox66 & lox71 sites is potentially interresting when the recombination reaction must be "irreversible". false false _141_ 0 1568 9 In stock false No modidification was made on the lox66 sequence true Eimad Shotar BBa_K318052_sequence 1 ttcgggtgtcaacaattgaccaaaatattgatttacagcgtaatgcgctttctagtgcaaattgtgaccgcattttgaagctt BBa_I718017_sequence 1 taccgttcgtatacgatacattatacgaagttat BBa_J44000_sequence 1 ttatcaaaaaccatggtttttgataa BBa_K318030_sequence 1 ataacttggtatagcatacattatacgaacggtatactagaggctagcactgtacctaggactgagctagccgtcaatactagagttatcaaaaaccatggtttttgataatactagagtaccgttcgtatacgatacattatacgaagttattactagaggctcttcaatctactagagaaaaaaaaaccccgcccctgacagggcggggtttttttttactagagaaaaaaaaaccccgcccctgacagggcggggtttttttttactagagttcgggtgtcaacaattgaccaaaatattgatttacagcgtaatgcgctttctagtgcaaattgtgaccgcattttgaagctttactagagttatcaaaaaccatggtttttgataa BBa_B1006_sequence 1 aaaaaaaaaccccgcccctgacagggcggggtttttttt BBa_I718016_sequence 1 ataacttggtatagcatacattatacgaacggta BBa_K318050_sequence 1 gctagcactgtacctaggactgagctagccgtcaa BBa_K318051_sequence 1 gctcttcaatc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z