LOCUS       BBa_K318519             3163 bp    DNA     linear       07-May-2024
DEFINITION  Bile Salt Induced Lysis Cassette
ACCESSION   BBa_K318519
VERSION     BBa_K318519.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     CDS             44..390
                     /label=BBa_K318516
     stem_loop       410..453
                     /label=stem_loop
     misc_feature    399..478
                     /label=BBa_B0010
     terminator      399..478
                     /label=BBa_B0010
     stem_loop       494..513
                     /label=T7 TE
     misc_feature    520..520
                     /label=stop
     polyA_site      514..527
                     /label=polya
     misc_feature    487..527
                     /label=BBa_B0012
     terminator      487..527
                     /label=BBa_B0012
     promoter        536..1370
                     /label=BBa_K318514
     CDS             1395..2051
                     /label=T4 holin
     CDS             1379..2054
                     /label=BBa_K112805
     CDS             2079..2573
                     /label=T4 Endolysin
     CDS             2063..2576
                     /label=BBa_K112806
     stem_loop       2596..2639
                     /label=stem_loop
     misc_feature    2585..2664
                     /label=BBa_B0010
     terminator      2585..2664
                     /label=BBa_B0010
     stem_loop       2680..2699
                     /label=T7 TE
     misc_feature    2706..2706
                     /label=stop
     polyA_site      2700..2713
                     /label=polya
     misc_feature    2673..2713
                     /label=BBa_B0012
     terminator      2673..2713
                     /label=BBa_B0012
     promoter        2722..2756
                     /label=BBa_J23116
     CDS             2779..3072
                     /label=T4 antiholin
     CDS             2765..3075
                     /label=BBa_K112807
     stem_loop       3095..3138
                     /label=stem_loop
     misc_feature    3084..3163
                     /label=BBa_B0010
     terminator      3084..3163
                     /label=BBa_B0010
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gaggatgacc atttccgctc
       61 aggttatcga cacgattgtc gagtggattg atgataattt gaatcagccg ttacgtattg
      121 atgatattgc ccgtcatgcg gggtattcca agtggcacct gcagcgcctt tttatgcagt
      181 acaaagggga gagtctggga cgctacgtgc gtgaacggaa gctaaaactg gcggcgcgcg
      241 acctgctcga caccgaccag aaggtgtatg atatttgtct caagtatggt tttgattcgc
      301 agcaaacctt tacgcgcatt tttacccgca cgttcaacct gccgccaggc gcttatcgta
      361 aagaaaagca tggccgtacg cattgaaatg tactagagcc aggcatcaaa taaaacgaaa
      421 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
      481 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagttatc
      541 agggggagcc gttgaccgtc gacgcgcgca gcgtcggaca caattgatac atctccagca
      601 ggatcgtgac gtaggcgcga gcttcttttt ttaaatcaaa cgattgcgga gcaaataacc
      661 agttttccat gagccctgaa ataaagctgc gcatcagtat cgccgcacga cgggtcagca
      721 gattttcagg cagcatttta gcattaatac aatgttttaa cgtttgttca atccgatcgt
      781 aactttccag acaaaggcta cgttgcgcct gttgaaccac aaccatttct ccgacaaact
      841 cacatttatg gaatataatc tccatcaata agcggcgtcg ttcttctgtt acagtagctt
      901 caagaatatg aactaaaatt tctcttaata cagatagtgg atcgtcgggg aattttgcct
      961 gatactcaat ctcaagctca ccaatattgg attctgatag ctcccagatc tcactgaata
     1021 aatccgactt atttttgaaa tgccaataga ttgcgccgcg cgtcacgcca gcagcgttcg
     1081 caatctccgc cagcgaggtt gccgatacgc cttgctgcga gaacaaacgc agggccacat
     1141 ccaggatgtg ttgtcgtgtc tccagcgctt gttgtttggt ttttcgtgcc atatgtcggt
     1201 gaatttacag gcgttagatt tacatacatt tatggatgta tgtaccatag cacgacgata
     1261 atataaacgc agcaatgggt ttaaggacct ttgaccattg accaatttga aatcggacac
     1321 tcgaggttta catatgaaca aaaacagagg gttaacgcct ctggcggtcg tactagagct
     1381 taaaaggagg gtctatggca gcacctagaa tatcattttc gccctctgat attctatttg
     1441 gtgttctcga tcgcttgttc aaagataacg ctaccgggaa ggttcttgct tcccgggtag
     1501 ctgtcgtaat tcttttgttt ataatggcga ttgtttggta taggggagat agtttctttg
     1561 agtactataa gcaatcaaag tatgaaacat acagtgaaat tattgaaaag gaaagaactg
     1621 cacgctttga atctgtcgcc ctggaacaac tccagatagt tcatatatca tctgaggcag
     1681 actttagtgc ggtgtattct ttccgcccta aaaacttaaa ctattttgtt gatattatag
     1741 catacgaagg aaaattacct tcaacaataa gtgaaaaatc acttggagga tatcctgttg
     1801 ataaaactat ggatgaatat acagttcatt taaatggacg tcattattat tccaactcaa
     1861 aatttgcttt tttaccaact aaaaagccta ctcccgaaat aaactacatg tacagttgtc
     1921 catattttaa tttggataat atctatgctg gaacgataac catgtactgg tatagaaatg
     1981 atcatataag taatgaccgc cttgaatcaa tatgtgctca ggcggccaga atattaggaa
     2041 gggctaaata attatactag agatacttag gaggtattat gaatatattt gaaatgttac
     2101 gtatagatga aggtcttaga cttaaaatct ataaagacac agaaggctat tacactattg
     2161 gcatcggtca tttgcttaca aaaagtccat cacttaatgc tgctaaatct gaattagata
     2221 aagctattgg gcgtaattgc aatggtgtaa ttacaaaaga tgaggctgaa aaactcttta
     2281 atcaggatgt tgatgctgct gttcgcggaa tcctgagaaa tgctaaatta aaaccggttt
     2341 atgattctct tgatgcggtt cgtcgctgtg cattgattaa tatggttttc caaatgggag
     2401 aaaccggtgt ggcaggattt actaactctt tacgtatgct tcaacaaaaa cgctgggatg
     2461 aagcagcagt taacttagct aaaagtagat ggtataatca aacacctaat cgcgcaaaac
     2521 gagtcattac aacgtttaga actggcactt gggacgcgta taaaaatcta taaagctact
     2581 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     2641 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     2701 ttctgcgttt atatactaga gttgacagct agctcagtcc tagggactat gctagctact
     2761 agaggatagg aggcctttat ggccttaaaa gcaacagcac tttttgccat gctaggattg
     2821 tcatttgttt tatctccatc gattgaagcg aatgtcgatc ctcattttga taaatttatg
     2881 gaatctggta ttaggcacgt ttatatgctt tttgaaaata aaagcgtaga atcgtctgaa
     2941 caattctata gttttatgag aacgacctat aaaaatgacc cgtgctcttc tgattttgaa
     3001 tgtatagagc gaggcgcgga gatggcacaa tcatacgcta gaattatgaa cattaaattg
     3061 gagactgaat gaaattacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     3121 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctc
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