LOCUS       BBa_K323001              803 bp    DNA     linear       19-May-2024
DEFINITION  cYFP link Zif268 under T7 promoter
ACCESSION   BBa_K323001
VERSION     BBa_K323001.1  
FEATURES             Location/Qualifiers
     promoter        1..17
                     /label=pT7
     misc_feature    20..44
                     /label=lacO
     RBS             76..82
                     /label=RBS
     misc_feature    88..90
                     /label=ATG
     misc_feature    91..111
                     /label=Tyr + His tag
     misc_feature    118..120
                     /label=ATG
     misc_feature    121..405
                     /label=BBa_K323032
     misc_feature    376..405
                     /label=linker 
     misc_feature    412..705
                     /label=BBa_K323105
     misc_feature    676..705
                     /label=linker
     misc_feature    712..729
                     /label=His tag
     misc_feature    730..732
                     /label=STOP
     misc_feature    734..803
                     /label=T7 term
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctgta gaaataattt
       61 tgtttaactt taagaaggag gtaaataatg tatcatcacc atcaccatca cactagaatg
      121 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
      181 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
      241 ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag
      301 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
      361 gacgagctgt acaagggtgg ctctggcggt gggagtgggg gaagcactag accgggtgaa
      421 aaaccgtatg catgtccggt tgaaagctgt gatcgtcgtt ttagccgtag tgatgaactg
      481 acccgtcata ttcgtattca tacaggtcag aaaccgtttc agtgtcgtat ttgcatgcgt
      541 aattttagcc gttcagatca tctgaccacc catattcgta cccatactgg cgaaaaaccg
      601 tttgcctgtg atatttgtgg tcgtaaattt gcacgttccg atgaacgtaa acgccatacc
      661 aaaattcata cgggtggtgg tagcggtggt ggttcaggtg gtagtactag acaccaccac
      721 caccaccact aaactagata actagcataa ccccttgggg cctctaaacg ggtcttgagg
      781 ggttttttga ctagtgcggc cgc
//