LOCUS       BBa_K323112             1785 bp    DNA     linear       19-May-2024
DEFINITION  pT7_TetO+NLS_TAL
ACCESSION   BBa_K323112
VERSION     BBa_K323112.1  
FEATURES             Location/Qualifiers
     promoter        1..18
                     /label=T7 promoter
     misc_feature    19..33
                     /label=TetO
     RBS             39..51
                     /label=rbs+Kozak sequence
     misc_feature    52..54
                     /label=start
     misc_feature    52..78
                     /label=NLS
     CDS             79..1785
                     /label=TAL protein
     misc_feature    1783..1785
                     /label=stop
ORIGIN
        1 taatacgact cactatagtc tatcaatgat agaactagaa ggagggccac catggatcca
       61 aaaaagaaga gaaaggtagg tccgcctctg caactggata caggtcagct gctgaaaatt
      121 gcaaaacgtg gtggtgttac cgcagttgaa gcagttcatg catggcgtaa tgcactgaca
      181 ggtgcaccgc tgaatctgac accggaacag gttgttgcaa ttgcaagcca tgatggtggt
      241 aaacaggcac tggaaaccgt tcagcgtctg ctgccggttc tgtgtcaggc acatggtctg
      301 acccctgaac aggtggtggc cattgccagt aatggtggtg gcaaacaggc gttagaaaca
      361 gtgcagcgcc tgctgcctgt tttatgccag gcccatggcc tgacaccaga gcaggtagtg
      421 gcgattgcga gcaatattgg cggtaaacaa gcccttgaaa ccgtgcaggc attactgccg
      481 gtgctgtgcc aagcgcacgg cctgacccca gaacaagttg ttgcgatcgc atcaaatggt
      541 ggcggtaagc aggctttgga aacggtgcag cgtttactgc cagtgttatg tcaggcgcat
      601 ggtttaacac cggaacaagt ggtggcaatc gcctcacatg atggcggaaa acaagcatta
      661 gagactgttc aacgcctgct gccagtgctt tgccaggcac acggcttaac acctgaacag
      721 gtcgtagcga ttgcatcaaa tattggtgga aaacaggcct tggagactgt acaggcactg
      781 ctgcctgtac tgtgtcaagc ccacggatta acgccagaac aggttgtggc tatcgccagc
      841 aattcaggcg gtaaacaggc gctcgagaca gttcaggccc tgctgccggt cttatgtcaa
      901 gctcacggac tgactcccga gcaggttgtc gccattgcct caaatggcgg tggtaaacaa
      961 gctctcgaaa cggtacagag actgctgccc gtcctgtgcc aggcgcatgg acttacgcct
     1021 gagcaagttg tggcaattgc atctaataat gggggtaagc aagcgctgga aacagttcaa
     1081 cgcttactgc ctgtcttgtg ccaagcacat ggtttaaccc cagagcaggt cgttgctatt
     1141 gcctctaaca ttggaggcaa acaggctctt gaaactgtcc aggccctgtt acctgttctg
     1201 tgccaggctc acggtttgac tccagagcaa gtggttgcaa tagccagcaa cggtgggggt
     1261 aaacaagctt tagaaaccgt ccaacgtctg ttaccagtgc tgtgtcaagc tcatggcctt
     1321 acacccgaac aagtagttgc cattgcgagt aacattggtg ggaagcaagc acttgaaacg
     1381 gttcaggcgc tgcttccagt attatgccag gcgcacggac tcactccaga acaggtagta
     1441 gcaatcgcaa gtaataacgg tgggaaacaa gcgttggaga cagtccaaag actgcttccg
     1501 gttctttgcc aagcccacgg tcttacaccg cagcaggttg tagctattgc tagtaatatt
     1561 ggaggtcgtc cggcactgga aagcattgtt gcacagctga gccgtcctga tccggcactg
     1621 gcagcactga ccaatgatca tctggttgca ctggcatgtc tgggtggtcg tcctgccctg
     1681 gatgcagtta aaaaaggtct gccgcatgca ccggcactga ttaaacgtac caatcgtcgt
     1741 atcccggaac gtaccagcca tcgtgttgca gatcatgcac agtaa
//