LOCUS       BBa_K323117              882 bp    DNA     linear       18-May-2024
DEFINITION  pT7_TetO+NLS_TetR_CL1_PEST
ACCESSION   BBa_K323117
VERSION     BBa_K323117.1  
FEATURES             Location/Qualifiers
     promoter        1..18
                     /label=T7 promoter
     misc_feature    19..33
                     /label=TetO
     RBS             39..51
                     /label=rbs+Kozak sequence
     misc_feature    52..54
                     /label=start
     misc_feature    55..78
                     /label=NLS
     misc_feature    880..882
                     /label=stop
     misc_feature    749..879
                     /label=PEST
     misc_feature    703..748
                     /label=CL1
     CDS             79..696
                     /label=Tet repressor
ORIGIN
        1 taatacgact cactatagtc tatcaatgat agaactagaa ggagggccac catggatcca
       61 aaaaagaaga gaaaggtatc cagattggat aaaagtaaag tgattaacag cgcattagag
      121 ctgcttaatg aggtcggaat cgaaggttta acaacccgta aactcgccca gaagctaggt
      181 gtagagcagc ctacattgta ttggcatgta aaaaataagc gggctttgct cgacgcctta
      241 gccattgaga tgttagatag gcaccatact cacttttgcc ctttagaagg ggaaagctgg
      301 caagattttt tacgtaataa cgctaaaagt tttagatgtg ctttactaag tcatcgcgat
      361 ggagcaaaag tacatttagg tacacggcct acagaaaaac agtatgaaac tctcgaaaat
      421 caattagcct ttttatgcca acaaggtttt tcactagaga atgcattata tgcactcagc
      481 gctgtggggc attttacttt aggttgcgta ttggaagatc aagagcatca agtcgctaaa
      541 gaagaaaggg aaacacctac tactgatagt atgccgccat tattacgaca agctatcgaa
      601 ttatttgatc accaaggtgc agagccagcc ttcttattcg gccttgaatt gatcatatgc
      661 ggattagaaa aacaacttaa atgtgaaagt gggtccacta gaaattctgc ttgcaagaac
      721 tggttcagta gcttaagcca ctttgtgatc caccttaaca gccacggctt ccctcccgag
      781 gtggaggagc aggccgccgg caccctgccc atgagctgcg cccaggagag cggcatggat
      841 agacaccctg ctgcttgcgc cagcgccagg atcaacgtct aa
//