LOCUS BBa_K323132 5535 bp DNA linear 10-Jun-2024 DEFINITION VioC, VioD and VioE enzymes fused with zinc fingers under pBAD promoter ACCESSION BBa_K323132 VERSION BBa_K323132.1 FEATURES Location/Qualifiers promoter 1..1209 /label=pBAD/araC RBS 1215..1220 /label=RBS CDS 1224..1787 /label=Gli1-linker CDS 1788..3087 /label=VioC RBS 3093..3098 /label=RBS CDS 3102..3392 /label=HivC-linker CDS 3393..4524 /label=VioD RBS 4530..4535 /label=RBS CDS 4830..5405 /label=VioE CDS 4539..4829 /label=PBSII-linker misc_feature 5406..5535 /label=double terminator ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat accgtttttt 1201 tgggctagcg aaagaggaga aatatgaagc gtgagcctga atctgtgtat gaaactgact 1261 gccgttggga tggctgtagc caggaatttg actcccaaga gcagctggtg caccacatca 1321 acagcgagca catccacggg gagcggaagg agttcgtgtg ccactggggg ggctgctcca 1381 gggagctgag gcccttcaaa gcccagtaca tgctggtggt tcacatgcgc agacacactg 1441 gcgagaagcc acacaagtgc acgtttgaag ggtgccggaa gtcatactca cgcctcgaaa 1501 acctgaagac gcacctgcgg tcacacacgg gtgagaagcc atacatgtgt gagcacgagg 1561 gctgtagtaa agccttcagc aatgccagtg accgagccaa gcaccagaat cggacccatt 1621 ccaatgagaa gccgtatgta tgtaagctcc ctggctgcac caaacgctat acagatccta 1681 gctcgctgcg aaaacatgtc aagacagtgc atggtcctga cgcccatgtg accaaacggc 1741 accgtgggga tactagaggc gggtcaggcg ggggctcagg cgggtcaatg aaacgtgcga 1801 ttatcgttgg tggcggcctg gcgggtggcc tgaccgcgat ctacctggcg aagcgtggct 1861 acgaagtgca cgtcgtggag aagcgtggtg atcctctgcg cgatctgagc tcttacgtgg 1921 acgttgttag cagccgtgcg atcggcgtga gcatgaccgt tcgtggtatc aagagcgttt 1981 tggctgcggg cattccgcgt gcagagctgg atgcgtgtgg cgaaccgatc gtggcaatgg 2041 ctttctccgt gggtggtcag tatcgcatgc gcgaactgaa gccgttggag gatttccgtc 2101 cgctgagctt gaaccgtgcg gcgtttcaaa agctgctgaa caaatacgcg aacctggcag 2161 gcgttcgtta ctactttgag cataagtgcc tggatgttga cctggatggt aagagcgtgt 2221 tgattcaggg caaagatggt cagccgcagc gtctgcaagg tgacatgatt atcggtgcgg 2281 atggcgccca cagcgccgtc cgtcaggcga tgcagagcgg cctgcgtcgt ttcgagttcc 2341 agcaaacgtt cttccgccat ggctacaaaa ccctggtttt gccggacgcg caagcactgg 2401 gttaccgtaa agacacgctg tactttttcg gcatggattc cggtggcctg ttcgcgggtc 2461 gtgcggctac gatcccagat ggtagcgtca gcatcgccgt ttgcctgccg tactcgggta 2521 gcccttccct gacgaccacc gacgaaccga cgatgcgtgc gttcttcgat cgttacttcg 2581 gtggcctgcc gcgtgacgcg cgtgacgaaa tgctgcgtca gtttctggcg aagccgagca 2641 acgacctgat taacgtgcgc tctagcacct ttcactataa gggtaatgtg ctgttgctgg 2701 gtgatgctgc gcatgcgact gcgccgttcc tgggtcaggg tatgaacatg gcgctggagg 2761 acgcccgcac gtttgtcgag ctgctggacc gccaccaggg cgaccaagac aaagcctttc 2821 cggagttcac ggagctgcgc aaagtccagg cagacgcaat gcaagacatg gctcgcgcca 2881 actatgacgt tttgagctgc tcgaacccga tctttttcat gcgtgcgcgt tacacgcgtt 2941 acatgcattc caagtttccg ggcctgtatc cgccggatat ggccgagaaa ctgtacttta 3001 cgagcgagcc gtacgatcgt ctgcaacaaa tccagcgtaa acagaatgtt tggtacaaga 3061 ttggtcgcgt gaattgaaga tctttaagaa agaggagaaa tatgcccttc cagtgtcgca 3121 tttgcatgcg gaacttttcg ctcaggacgg accttgacag gcatacccgt actcataccg 3181 gtgaaaaacc gtttcagtgt cggatctgta tgcgaaattt ctccctcagc cagaccttgc 3241 gccgccatct acgtacgcac accggcgaga agccattcca atgccgaata tgcatgcgca 3301 acttcagtct caggagcaac ctggggcggc acctaaaaac ccacacagga gaaaaaacta 3361 gaggcgggtc aggcgggggc tcaggcgggt caatgaagat tctggtcatt ggtgctggtc 3421 cagctggtct ggttttcgca tcccaactga agcaggcacg ccctttgtgg gccattgaca 3481 tcgtggagaa gaatgacgag caagaagtgc tgggctgggg tgtcgtgctg cctggccgtc 3541 cgggtcagca cccggcgaac ccgctgtcct atctggatgc accggagcgt ctgaatccgc 3601 aatttctgga ggacttcaaa ctggtgcatc ataatgagcc gtccttgatg tccacgggcg 3661 ttttgttgtg cggcgtggag cgtcgcggtc tggttcacgc gctgcgcgat aagtgccgca 3721 gccaaggcat tgctattcgt ttcgaaagcc cgttgctgga acacggtgag ctgccgctgg 3781 cggactatga tctggtggtc ctggctaatg gtgttaatca caaaaccgcg catttcaccg 3841 aggctctggt cccgcaggtg gactacggcc gcaataagta catttggtat ggcactagcc 3901 agctgttcga tcagatgaat ctggtttttc gtacccatgg taaagatatc tttatcgcgc 3961 atgcctataa gtatagcgat accatgagca cgttcattgt cgaatgtagc gaagagactt 4021 acgcacgcgc acgcctgggc gaaatgtccg aagaggcgag cgcagaatac gttgctaagg 4081 tgttccaggc cgagctgggt ggtcacggcc tggtgagcca gccgggtctg ggttggcgta 4141 acttcatgac gttgtctcat gaccgttgtc atgatggtaa gttggttctg ctgggtgacg 4201 cgctgcaaag cggtcacttt agcatcggcc acggcaccac gatggccgtg gtggtggcgc 4261 agctgctggt taaagcgctg tgtaccgaag atggtgtgcc tgccgcgctg aaacgtttcg 4321 aagagcgtgc cctgccgctg gtgcagttgt tccgtggcca cgcagacaac agccgcgttt 4381 ggttcgaaac cgtcgaagag cgcatgcacc tgtcctcggc ggaatttgtg caaagcttcg 4441 acgcacgccg caaaagcctg ccgccgatgc cggaagcact ggcgcagaat ctgcgttatg 4501 ctttgcagcg ctgatgatca ttaagaaaga ggagaaatat gccgggtgaa aaaccgtatg 4561 catgtcctga atgtggtaaa agctttagcc agcgtgcaaa tctgcgtgca catcagcgta 4621 cccatacagg tgaaaagcct tataaatgcc cagaatgcgg caaaagcttt tcacgtagcg 4681 atcatctgac cacccatcag cgcacacata ctggcgagaa accttacaaa tgtccagagt 4741 gtggtaaatc atttagccgt agtgatgttc tggttcgtca tcagagaacc cacacgggtg 4801 gtggtagcgg tggtggttca ggtggtagta tggagaaccg tgagccacca ctgttgccag 4861 cccgttggag cagcgcctat gtctcttatt ggagcccgat gctgccggat gaccagctga 4921 ccagcggcta ttgctggttc gactatgaac gtgacatctg tcgtattgac ggcctgttca 4981 atccgtggag cgagcgtgat actggttatc gcctgtggat gtcggaggtt ggtaatgcgg 5041 ccagcggccg tacctggaaa caaaaagtcg cctatggtcg tgagcgtacc gccctgggtg 5101 aacagctgtg tgagcgtccg ctggatgatg agactggccc ttttgccgaa ttgttcctgc 5161 cacgcgatgt cctgcgccgt ctgggtgccc gtcacattgg ccgtcgcgtg gttctgggtc 5221 gcgaagcgga cggttggcgt taccagcgcc caggtaaagg tccgagcacc ctgtacctgg 5281 atgcggcgag cggcactcca ctgcgcatgg tcaccggcga tgaagcgtcg cgtgcaagcc 5341 tgcgtgattt tccgaatgtg agcgaggcgg agatcccgga cgcggttttc gcggccaagc 5401 gctaaccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 5461 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcgacct tcgggtgggc 5521 ctttctgcgt ttata //