LOCUS BBa_K325100 2626 bp DNA linear 07-May-2024
DEFINITION EPIC Firefly Luciferase and LRE
P. Pyralis
(E. coli
optimised)
ACCESSION BBa_K325100
VERSION BBa_K325100.1
FEATURES Location/Qualifiers
RBS 1..17
/label=rbs
CDS 18..941
/label=Luciferin regenerating enzyme (P.pyralis)
misc_feature 942..947
/label=double stop codon
misc_feature 948..953
/label=BamH1 site
RBS 954..970
/label=rbs
misc_feature 971..2620
/label=EPIC luciferase (P. pyralis)
misc_feature 2621..2626
/label=double stop codon
ORIGIN
1 ttaaggaggt aaaaaaaatg ggtccggtcg ttgaaaagat cgccgagctg ggtaagtaca
61 cggttggtga gggtccgcac tgggaccacg aaacgcagac gctgtatttc gtggatacgg
121 tcgagaaaac gttccacaaa tacgttccga gccagaagaa atacacgttt tgtaaggtgg
181 acaagctggt tagctttatc attccgctgg caggttctcc gggtcgcttc gtcgtcagct
241 tggagcgcga gattgcgatc ctgacgtggg acggcgtcag cgcggcaccg accagcattg
301 aggcgattgt gaatgtcgag ccgcacatca agaataatcg cctgaacgac ggtaaagcgg
361 acccgctggg taatctgtgg accggcacga tggccattga tgcgggtttg ccggtgggtc
421 cggtgaccgg ctccctgtac cacctgggtg cggataagaa ggtcaagatg catgaaagca
481 atattgccat cgctaacggc ctggcgtgga gcaacgacct gaagaagatg tactatattg
541 acagcggcaa acgtcgtgtt gacgaatacg actatgatgc gagcacgctg tctatcagca
601 atcagcgtcc gctgttcacc ttcgaaaaac atgaggtccc tggctaccca gacggtcaaa
661 ccattgacga agaaggtaac ctgtgggtcg ccgtgtttca aggccagcgt attatcaaga
721 ttagcaccca gcagccggag gttctgctgg ataccgtcaa aatcccggac ccgcaggtga
781 ctagcgttgc attcggtggt ccgaatctgg acgagctgca tgtgaccagc gccggtttgc
841 agctggatga cagctccctg gacaaatctt tggtcaatgg tcacgtctac cgtgtgactg
901 gcctgggtgt taagggtttc gccggtgtga aagttaagct gtaataagga tccttaagga
961 ggtaaaaaaa atggaggacg cgaaaaacat caagaaaggc ccagcaccgt tctatccgct
1021 ggaagatggt accgccggtg aacaattgca caaagcgatg aaacgttatg cgctggttcc
1081 gggtaccatc gcattcacgg acgcgcatat cgaggttaac atcacctatg ccgagtactt
1141 cgagatgagc gttcgtctgg ccgaggcaat gaagcgttat ggtttgaata ccaatcatcg
1201 catcgtcgtc tgtagcgaga acagcttgca gtttttcatg ccagttctgg gtgcactgtt
1261 tattggtgtg gcagtggcgc ctgcgaatga tatctacaac gagcgtgaac tgttgaatag
1321 catgaacatt tcgcagccga cggttgtttt tgtgtccaag aaaggtttgc agaagattct
1381 gaatgtgcaa aagaagttgc cgatcattca gaaaatcatc atcatggaca gcaagaccga
1441 ttaccagggt ttccaaagca tgtacacttt tgtgacgagc cacctgcctc cgggttttaa
1501 cgagtatgat ttcgttccgg aaagcttcga ccgtgataag acgattgcac tgattatgaa
1561 ctcgtccggc tctaccggcc tgcctaaagg cgtggccctg ccgcaccgta ctgcatgtgt
1621 ccgcttcagc cacgcgcgtg atccgatctt cggtaaccaa atcattccgg acaccgccat
1681 tttgtctgtc gttccgtttc atcatggctt tggtatgttt accactctgg gttacctgat
1741 ctgtggtttc cgtgttgttc tgatgtaccg tttcgaagaa gaactgttcc tgcgtagcct
1801 gcaagattac aagatccaat ccgcgctgct ggtgccgacg ctgtttagct tcttcgctaa
1861 atccaccctg attgacaaat atgacttgag caacctgcac gaaatcgcgt cgggcggtgc
1921 gccgctgagc aaagaagtcg gtgaggccgt tgcgaaacgt ttccatctgc cgggcatccg
1981 tcagggttat ggcctgacgg aaacgaccag cgcgattttg attacccctg agggtgacga
2041 taaaccgggt gccgttggca aggtggtacc gttctttgag gctaaagtcg tggacctgga
2101 cacgggtaag actctgggtg tgaatcaacg tggcgagttg tgcgttcgtg gcccgatgat
2161 catgagcggt tatgtcaaca acccggaagc aactaatgcg ttgatcgata aggatggctg
2221 gctgcacagc ggcgacctgg cgtactggga tgaggatgaa cactttttca tcgttggtcg
2281 cctgaagagc ctgatcaagt acaagggcta ccaggtggca ccggctgaac tggagagcat
2341 tctgttgcaa caccctaata tcttcgacgc gggtgttgcg ggtctgcctg atgatgatgc
2401 cggtgaattg ccagctgcgg tggtcgttct ggaacacggt aaaacgatga ctgaaaaaga
2461 aatcgtggac tacgtcgcct cgcaggtgac caccgctaaa aagttgcgtg gtggcgtggt
2521 gtttgtggat gaagtgccga aaggcctgac gggtaaacgt gatgcacgta agattcgtga
2581 gatcttgatc aaggcgaaga aaggtggtaa atccaagttg taataa
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