BBa_K325100
1
BBa_K325100
EPIC Firefly Luciferase and LRE <BR><i>P. Pyralis<BR>(E. coli optimised)</i>
2010-09-14T11:00:00Z
2015-05-08T01:12:02Z
Codon optimised and synthesised by DNA 2.0
Released HQ 2013
This is a mutant luciferase that is very much brighter than wild-type. It is supplied in an operon with an enzyme (LRE) that regenerates its substrate. When supplied with luciferin and placed under a promoter it will emit light at a green wavelength. If D-cysteine is added the LRE will be able to regenerate the luciferin substrate.
false
false
_446_
0
7466
9
In stock
false
It was ensured that no unwanted restriction sites were created during optimisation. A BamH1 site was added between the two genes.
false
Ben Reeve and Paul Masset
annotation2080157
1
Luciferin regenerating enzyme (P.pyralis)
range2080157
1
18
941
annotation2080156
1
rbs
range2080156
1
1
17
annotation2080158
1
double stop codon
range2080158
1
942
947
annotation2080162
1
double stop codon
range2080162
1
2621
2626
annotation2080159
1
BamH1 site
range2080159
1
948
953
annotation2080161
1
EPIC luciferase (P. pyralis)
range2080161
1
971
2620
annotation2080160
1
rbs
range2080160
1
954
970
BBa_K325100_sequence
1
ttaaggaggtaaaaaaaatgggtccggtcgttgaaaagatcgccgagctgggtaagtacacggttggtgagggtccgcactgggaccacgaaacgcagacgctgtatttcgtggatacggtcgagaaaacgttccacaaatacgttccgagccagaagaaatacacgttttgtaaggtggacaagctggttagctttatcattccgctggcaggttctccgggtcgcttcgtcgtcagcttggagcgcgagattgcgatcctgacgtgggacggcgtcagcgcggcaccgaccagcattgaggcgattgtgaatgtcgagccgcacatcaagaataatcgcctgaacgacggtaaagcggacccgctgggtaatctgtggaccggcacgatggccattgatgcgggtttgccggtgggtccggtgaccggctccctgtaccacctgggtgcggataagaaggtcaagatgcatgaaagcaatattgccatcgctaacggcctggcgtggagcaacgacctgaagaagatgtactatattgacagcggcaaacgtcgtgttgacgaatacgactatgatgcgagcacgctgtctatcagcaatcagcgtccgctgttcaccttcgaaaaacatgaggtccctggctacccagacggtcaaaccattgacgaagaaggtaacctgtgggtcgccgtgtttcaaggccagcgtattatcaagattagcacccagcagccggaggttctgctggataccgtcaaaatcccggacccgcaggtgactagcgttgcattcggtggtccgaatctggacgagctgcatgtgaccagcgccggtttgcagctggatgacagctccctggacaaatctttggtcaatggtcacgtctaccgtgtgactggcctgggtgttaagggtttcgccggtgtgaaagttaagctgtaataaggatccttaaggaggtaaaaaaaatggaggacgcgaaaaacatcaagaaaggcccagcaccgttctatccgctggaagatggtaccgccggtgaacaattgcacaaagcgatgaaacgttatgcgctggttccgggtaccatcgcattcacggacgcgcatatcgaggttaacatcacctatgccgagtacttcgagatgagcgttcgtctggccgaggcaatgaagcgttatggtttgaataccaatcatcgcatcgtcgtctgtagcgagaacagcttgcagtttttcatgccagttctgggtgcactgtttattggtgtggcagtggcgcctgcgaatgatatctacaacgagcgtgaactgttgaatagcatgaacatttcgcagccgacggttgtttttgtgtccaagaaaggtttgcagaagattctgaatgtgcaaaagaagttgccgatcattcagaaaatcatcatcatggacagcaagaccgattaccagggtttccaaagcatgtacacttttgtgacgagccacctgcctccgggttttaacgagtatgatttcgttccggaaagcttcgaccgtgataagacgattgcactgattatgaactcgtccggctctaccggcctgcctaaaggcgtggccctgccgcaccgtactgcatgtgtccgcttcagccacgcgcgtgatccgatcttcggtaaccaaatcattccggacaccgccattttgtctgtcgttccgtttcatcatggctttggtatgtttaccactctgggttacctgatctgtggtttccgtgttgttctgatgtaccgtttcgaagaagaactgttcctgcgtagcctgcaagattacaagatccaatccgcgctgctggtgccgacgctgtttagcttcttcgctaaatccaccctgattgacaaatatgacttgagcaacctgcacgaaatcgcgtcgggcggtgcgccgctgagcaaagaagtcggtgaggccgttgcgaaacgtttccatctgccgggcatccgtcagggttatggcctgacggaaacgaccagcgcgattttgattacccctgagggtgacgataaaccgggtgccgttggcaaggtggtaccgttctttgaggctaaagtcgtggacctggacacgggtaagactctgggtgtgaatcaacgtggcgagttgtgcgttcgtggcccgatgatcatgagcggttatgtcaacaacccggaagcaactaatgcgttgatcgataaggatggctggctgcacagcggcgacctggcgtactgggatgaggatgaacactttttcatcgttggtcgcctgaagagcctgatcaagtacaagggctaccaggtggcaccggctgaactggagagcattctgttgcaacaccctaatatcttcgacgcgggtgttgcgggtctgcctgatgatgatgccggtgaattgccagctgcggtggtcgttctggaacacggtaaaacgatgactgaaaaagaaatcgtggactacgtcgcctcgcaggtgaccaccgctaaaaagttgcgtggtggcgtggtgtttgtggatgaagtgccgaaaggcctgacgggtaaacgtgatgcacgtaagattcgtgagatcttgatcaaggcgaagaaaggtggtaaatccaagttgtaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z