BBa_K325100 1 BBa_K325100 EPIC Firefly Luciferase and LRE <BR><i>P. Pyralis<BR>(E. coli optimised)</i> 2010-09-14T11:00:00Z 2015-05-08T01:12:02Z Codon optimised and synthesised by DNA 2.0 Released HQ 2013 This is a mutant luciferase that is very much brighter than wild-type. It is supplied in an operon with an enzyme (LRE) that regenerates its substrate. When supplied with luciferin and placed under a promoter it will emit light at a green wavelength. If D-cysteine is added the LRE will be able to regenerate the luciferin substrate. false false _446_ 0 7466 9 In stock false It was ensured that no unwanted restriction sites were created during optimisation. A BamH1 site was added between the two genes. false Ben Reeve and Paul Masset annotation2080157 1 Luciferin regenerating enzyme (P.pyralis) range2080157 1 18 941 annotation2080156 1 rbs range2080156 1 1 17 annotation2080158 1 double stop codon range2080158 1 942 947 annotation2080162 1 double stop codon range2080162 1 2621 2626 annotation2080159 1 BamH1 site range2080159 1 948 953 annotation2080161 1 EPIC luciferase (P. pyralis) range2080161 1 971 2620 annotation2080160 1 rbs range2080160 1 954 970 BBa_K325100_sequence 1 ttaaggaggtaaaaaaaatgggtccggtcgttgaaaagatcgccgagctgggtaagtacacggttggtgagggtccgcactgggaccacgaaacgcagacgctgtatttcgtggatacggtcgagaaaacgttccacaaatacgttccgagccagaagaaatacacgttttgtaaggtggacaagctggttagctttatcattccgctggcaggttctccgggtcgcttcgtcgtcagcttggagcgcgagattgcgatcctgacgtgggacggcgtcagcgcggcaccgaccagcattgaggcgattgtgaatgtcgagccgcacatcaagaataatcgcctgaacgacggtaaagcggacccgctgggtaatctgtggaccggcacgatggccattgatgcgggtttgccggtgggtccggtgaccggctccctgtaccacctgggtgcggataagaaggtcaagatgcatgaaagcaatattgccatcgctaacggcctggcgtggagcaacgacctgaagaagatgtactatattgacagcggcaaacgtcgtgttgacgaatacgactatgatgcgagcacgctgtctatcagcaatcagcgtccgctgttcaccttcgaaaaacatgaggtccctggctacccagacggtcaaaccattgacgaagaaggtaacctgtgggtcgccgtgtttcaaggccagcgtattatcaagattagcacccagcagccggaggttctgctggataccgtcaaaatcccggacccgcaggtgactagcgttgcattcggtggtccgaatctggacgagctgcatgtgaccagcgccggtttgcagctggatgacagctccctggacaaatctttggtcaatggtcacgtctaccgtgtgactggcctgggtgttaagggtttcgccggtgtgaaagttaagctgtaataaggatccttaaggaggtaaaaaaaatggaggacgcgaaaaacatcaagaaaggcccagcaccgttctatccgctggaagatggtaccgccggtgaacaattgcacaaagcgatgaaacgttatgcgctggttccgggtaccatcgcattcacggacgcgcatatcgaggttaacatcacctatgccgagtacttcgagatgagcgttcgtctggccgaggcaatgaagcgttatggtttgaataccaatcatcgcatcgtcgtctgtagcgagaacagcttgcagtttttcatgccagttctgggtgcactgtttattggtgtggcagtggcgcctgcgaatgatatctacaacgagcgtgaactgttgaatagcatgaacatttcgcagccgacggttgtttttgtgtccaagaaaggtttgcagaagattctgaatgtgcaaaagaagttgccgatcattcagaaaatcatcatcatggacagcaagaccgattaccagggtttccaaagcatgtacacttttgtgacgagccacctgcctccgggttttaacgagtatgatttcgttccggaaagcttcgaccgtgataagacgattgcactgattatgaactcgtccggctctaccggcctgcctaaaggcgtggccctgccgcaccgtactgcatgtgtccgcttcagccacgcgcgtgatccgatcttcggtaaccaaatcattccggacaccgccattttgtctgtcgttccgtttcatcatggctttggtatgtttaccactctgggttacctgatctgtggtttccgtgttgttctgatgtaccgtttcgaagaagaactgttcctgcgtagcctgcaagattacaagatccaatccgcgctgctggtgccgacgctgtttagcttcttcgctaaatccaccctgattgacaaatatgacttgagcaacctgcacgaaatcgcgtcgggcggtgcgccgctgagcaaagaagtcggtgaggccgttgcgaaacgtttccatctgccgggcatccgtcagggttatggcctgacggaaacgaccagcgcgattttgattacccctgagggtgacgataaaccgggtgccgttggcaaggtggtaccgttctttgaggctaaagtcgtggacctggacacgggtaagactctgggtgtgaatcaacgtggcgagttgtgcgttcgtggcccgatgatcatgagcggttatgtcaacaacccggaagcaactaatgcgttgatcgataaggatggctggctgcacagcggcgacctggcgtactgggatgaggatgaacactttttcatcgttggtcgcctgaagagcctgatcaagtacaagggctaccaggtggcaccggctgaactggagagcattctgttgcaacaccctaatatcttcgacgcgggtgttgcgggtctgcctgatgatgatgccggtgaattgccagctgcggtggtcgttctggaacacggtaaaacgatgactgaaaaagaaatcgtggactacgtcgcctcgcaggtgaccaccgctaaaaagttgcgtggtggcgtggtgtttgtggatgaagtgccgaaaggcctgacgggtaaacgtgatgcacgtaagattcgtgagatcttgatcaaggcgaagaaaggtggtaaatccaagttgtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z