LOCUS       BBa_K327001             2331 bp    DNA     linear       17-May-2024
DEFINITION  RBS+CI+RBS+RFP+F1610
ACCESSION   BBa_K327001
VERSION     BBa_K327001.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             22..729
                     /label=cI lambda
     misc_feature    730..762
                     /label=LVA
     misc_feature    769..793
                     /label=Help:Barcodes
     CDS             19..793
                     /label=BBa_C0051
     misc_feature    806..809
                     /label=conserved
     RBS             802..813
                     /label=BBa_B0034
     CDS             820..1494
                     /label=mrfp1
     misc_feature    1501..1525
                     /label=Help:Barcodes
     CDS             820..1525
                     /label=BBa_E1010
     misc_feature    1538..1541
                     /label=conserved
     RBS             1534..1545
                     /label=BBa_B0034
     misc_feature    2131..2162
                     /label=LVA
     CDS             1552..2130
                     /label=luxI
     misc_feature    1552..2169
                     /label=BBa_C0061
     misc_feature    2170..2194
                     /label=Help:Barcodes
     CDS             1552..2169
                     /label=BBa_C0061
     stem_loop       2214..2257
                     /label=stem_loop
     misc_feature    2203..2282
                     /label=BBa_B0010
     terminator      2203..2282
                     /label=BBa_B0010
     stem_loop       2298..2317
                     /label=T7 TE
     misc_feature    2324..2324
                     /label=stop
     polyA_site      2318..2331
                     /label=polya
     misc_feature    2291..2331
                     /label=BBa_B0012
     terminator      2291..2331
                     /label=BBa_B0012
     misc_feature    1534..2331
                     /label=BBa_F1610
ORIGIN
        1 aaagaggaga aatactagat gagcacaaaa aagaaaccat taacacaaga gcagcttgag
       61 gacgcacgtc gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg cttatcccag
      121 gaatctgtcg cagacaagat ggggatgggg cagtcaggcg ttggtgcttt atttaatggc
      181 atcaatgcat taaatgctta taacgccgca ttgcttgcaa aaattctcaa agttagcgtt
      241 gaagaattta gcccttcaat cgccagagaa atctacgaga tgtatgaagc ggttagtatg
      301 cagccgtcac ttagaagtga gtatgagtac cctgtttttt ctcatgttca ggcagggatg
      361 ttctcacctg agcttagaac ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc
      421 aaaaaagcca gtgattctgc attctggctt gaggttgaag gtaattccat gaccgcacca
      481 acaggctcca agccaagctt tcctgacgga atgttaattc tcgttgaccc tgagcaggct
      541 gttgagccag gtgatttctg catagccaga cttgggggtg atgagtttac cttcaagaaa
      601 ctgatcaggg atagcggtca ggtgttttta caaccactaa acccacagta cccaatgatc
      661 ccatgcaatg agagttgttc cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag
      721 acgtttggcg ctgcaaacga cgaaaactac gctttagtag cttaataacg ctgatagtgc
      781 tagtgtagat cgctactaga gaaagaggag aaatactaga tggcttcctc cgaagacgtt
      841 atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg tcacgagttc
      901 gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc taaactgaaa
      961 gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca gttccagtac
     1021 ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa actgtccttc
     1081 ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt tgttaccgtt
     1141 acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact gcgtggtacc
     1201 aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga agcttccacc
     1261 gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg
     1321 aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa aaaaccggtt
     1381 cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca caacgaagac
     1441 tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa
     1501 cgctgatagt gctagtgtag atcgctacta gagaaagagg agaaatacta gatgactata
     1561 atgataaaaa aatcggattt tttggcaatt ccatcggagg agtataaagg tattctaagt
     1621 cttcgttatc aagtgtttaa gcaaagactt gagtgggact tagttgtaga aaataacctt
     1681 gaatcagatg agtatgataa ctcaaatgca gaatatattt atgcttgtga tgatactgaa
     1741 aatgtaagtg gatgctggcg tttattacct acaacaggtg attatatgct gaaaagtgtt
     1801 tttcctgaat tgcttggtca acagagtgct cccaaagatc ctaatatagt cgaattaagt
     1861 cgttttgctg taggtaaaaa tagctcaaag ataaataact ctgctagtga aattacaatg
     1921 aaactatttg aagctatata taaacacgct gttagtcaag gtattacaga atatgtaaca
     1981 gtaacatcaa cagcaataga gcgattttta aagcgtatta aagttccttg tcatcgtatt
     2041 ggagacaaag aaattcatgt attaggtgat actaaatcgg ttgtattgtc tatgcctatt
     2101 aatgaacagt ttaaaaaagc agtcttaaat gctgcaaacg acgaaaacta cgctttagta
     2161 gcttaataac tctgatagtg ctagtgtaga tctctactag agccaggcat caaataaaac
     2221 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     2281 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
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