LOCUS       BBa_K327016             2875 bp    DNA     linear       29-May-2024
DEFINITION  Lux activated, C1lam repressed switch
ACCESSION   BBa_K327016
VERSION     BBa_K327016.1  
FEATURES             Location/Qualifiers
     misc_feature    1..12
                     /label=B0034
     misc_feature    19..19
                     /label=PSP3 pag
     CDS             19..237
                     /label=PSP3 pag
     misc_feature    1..237
                     /label=BBa_I746351
     misc_feature    250..253
                     /label=conserved
     RBS             246..257
                     /label=BBa_B0034
     CDS             264..866
                     /label=LasI
     misc_feature    867..899
                     /label=LVA
     misc_feature    906..930
                     /label=Help:Barcodes
     CDS             264..905
                     /label=BBa_C0078
     misc_feature    939..1035
                     /label=BBa_R0065
     misc_feature    944..963
                     /label=Lux Box
     promoter        985..990
                     /label=-10
     misc_feature    995..1011
                     /label=OR2 cI
     promoter        996..996
                     /label=lux p(R) start
     promoter        1009..1014
                     /label=-35
     misc_feature    1019..1035
                     /label=OR1 cI
     promoter        1032..1035
                     /label=-10
     promoter        939..1035
                     /label=BBa_R0065
     misc_feature    1048..1051
                     /label=conserved
     RBS             1044..1055
                     /label=BBa_B0034
     CDS             1062..1730
                     /label=cI 434
     misc_feature    1692..1730
                     /label=LVA
     misc_feature    1062..1730
                     /label=BBa_C0052
     misc_feature    1731..1755
                     /label=Help:Barcodes
     CDS             1062..1730
                     /label=BBa_C0052
     misc_feature    1764..1775
                     /label=B0034
     CDS             1785..2027
                     /label=phiR73 delta
     misc_feature    1785..1785
                     /label=phiR73 delta
     misc_feature    1865..1865
                     /label=silent mutation to remove PstI site
     misc_feature    1764..2027
                     /label=BBa_I746352
     misc_feature    2040..2043
                     /label=conserved
     RBS             2036..2047
                     /label=BBa_B0034
     CDS             2057..2673
                     /label=tetR
     misc_feature    2674..2707
                     /label=SsrA
     misc_feature    2714..2738
                     /label=Help:Barcodes
     CDS             2054..2713
                     /label=BBa_C0040
     stem_loop       2758..2801
                     /label=stem_loop
     misc_feature    2747..2826
                     /label=BBa_B0010
     terminator      2747..2826
                     /label=BBa_B0010
     stem_loop       2842..2861
                     /label=T7 TE
     misc_feature    2868..2868
                     /label=stop
     polyA_site      2862..2875
                     /label=polya
     misc_feature    2835..2875
                     /label=BBa_B0012
     terminator      2835..2875
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gatgcactgc ccgttatgcc aaaacgctgc acatgctcgc
       61 actagccggt accttagcac cgaaacgaaa gaacgttatc accagtgcca aaacataaat
      121 tgcggatgta catttatcac ttttgagaca ctatcaagat tcattgtgaa accggggact
      181 gttgatcctg ctccgcccca ccccatcaga aaccaacaac agcaactttg gctttgatac
      241 tagagaaaga ggagaaatac tagatgatcg ttcagatcgg tcgtcgtgaa gagttcgaca
      301 aaaaactgct gggtgaaatg cacaaactgc gtgctcaggt tttcaaagaa cgtaaaggtt
      361 gggacgtttc cgttatcgac gaaatggaaa tcgacggtta cgacgctctg tccccgtact
      421 acatgctgat ccaggaagac accccggaag ctcaggtttt cggttgctgg cgtatcttcg
      481 acaccaccgg tccgtacatg ctgaaaaaca ccttcccgga actgctgcac ggtaaagaag
      541 ctccgtgctc cccgcacatc tgggaactgt cccgtttcgc tatcaactcc ggtcagaaag
      601 gttccctggg tttctccgac tgcaccctgg aagctatgcg tgctctggct cgttactcct
      661 tgcagaacga catccagacc ctggttaccg ttaccaccgt tggtgttgaa aaaatgatga
      721 tccgtgctgg tctggacgtt tcccgtttcg gtccgcacct gaaaatcggt atcgaacgtg
      781 ctgttgctct gcgtatcgaa ctgaacgcta aaacccagat cgctctgtac ggtggtgttc
      841 tggttgaaca gcgtctggct gtttccgctg ctaacgacga aaactacgct ctggttgctt
      901 aataactctg atagtgctag tgtagatctc tactagagta agcacctgta ggatcgtaca
      961 ggtttacgca agaaaatggt ttgttatagt cgaataacac cgtgcgtgtt gactatttta
     1021 cctctggcgg tgatatacta gagaaagagg agaaatacta gatgagtatt tcttccaggg
     1081 taaaaagcaa aagaatccag cttggactta accaggctga acttgctcaa aaggtgggga
     1141 ctacccagca gtctatagag cagctcgaaa acggtaaaac taagcgacca cgctttttac
     1201 cagaacttgc gtcagctctt ggcgtaagtg ttgactggct gctcaatggc acctctgatt
     1261 cgaatgttag atttgttggg cacgttgagc ccaaagggaa atatccattg attagcatgg
     1321 ttagagctgg ttcgtggtgt gaagcttgtg aaccctacga tatcaaggac attgatgaat
     1381 ggtatgacag tgacgttaac ttattaggca atggattctg gctgaaggtt gaaggtgatt
     1441 ccatgacctc acctgtaggt caaagcatcc ctgaaggtca tatggtgtta gtagatactg
     1501 gacgggagcc agtgaatgga agccttgttg tagccaaact gactgacgcg aacgaagcaa
     1561 cattcaagaa actggtcata gatggcggtc agaagtacct gaaaggcctg aatccttcat
     1621 ggcctatgac tcctatcaac ggaaactgca agattatcgg tgttgtcgtg gaagcgaggg
     1681 taaaattcgt agctgcaaac gacgaaaact acgctttagt agcttaataa ctctgatagt
     1741 gctagtgtag atctctacta gagaaagagg agaaatacta gatgatgcgc tgccctttct
     1801 gtcgtcattc agcgcatacc cgcaccagcc ggtatgtgag tgacaatgtc aaagaaagtt
     1861 atctccagtg ccagaatatt tactgttcgg cgacatttaa aacgcatgag tcaatttgtg
     1921 ccgtgattcg ttctccggtc acggaggaaa aaccagcacc ggcaagcaca gcaccggctg
     1981 ttgtccgaaa agttaaaggc tgttacagct caccattcaa ccattaatac tagagaaaga
     2041 ggagaaatac tagatgtcca gattagataa aagtaaagtg attaacagcg cattagagct
     2101 gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt
     2161 agagcagcct acattgtatt ggcatgtaaa aaataagcgg gctttgctcg acgccttagc
     2221 cattgagatg ttagataggc accatactca cttttgccct ttagaagggg aaagctggca
     2281 agatttttta cgtaataacg ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg
     2341 agcaaaagta catttaggta cacggcctac agaaaaacag tatgaaactc tcgaaaatca
     2401 attagccttt ttatgccaac aaggtttttc actagagaat gcattatatg cactcagcgc
     2461 tgtggggcat tttactttag gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga
     2521 agaaagggaa acacctacta ctgatagtat gccgccatta ttacgacaag ctatcgaatt
     2581 atttgatcac caaggtgcag agccagcctt cttattcggc cttgaattga tcatatgcgg
     2641 attagaaaaa caacttaaat gtgaaagtgg gtccgctgca aacgacgaaa actacgcttt
     2701 agtagcttaa taacactgat agtgctagtg tagatcacta ctagagccag gcatcaaata
     2761 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     2821 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
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