LOCUS       BBa_K329053             2072 bp    DNA     linear       29-May-2024
DEFINITION  [KanR+LacZ] in between WT Tn916 Arms
ACCESSION   BBa_K329053
VERSION     BBa_K329053.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     misc_feature    80..95
                     /label=Rigth Xis binding site
     misc_feature    44..233
                     /label=BBa_K329041
     promoter        242..276
                     /label=BBa_J23110
     misc_feature    289..292
                     /label=conserved
     RBS             285..296
                     /label=BBa_B0034
     CDS             303..536
                     /label=lacZ
     misc_feature    303..305
                     /label=START
     misc_feature    531..536
                     /label=STOP
     CDS             303..536
                     /label=BBa_I732006
     stem_loop       556..599
                     /label=stem_loop
     misc_feature    545..624
                     /label=BBa_B0010
     terminator      545..624
                     /label=BBa_B0010
     stem_loop       640..659
                     /label=T7 TE
     misc_feature    666..666
                     /label=stop
     polyA_site      660..673
                     /label=polya
     misc_feature    633..673
                     /label=BBa_B0012
     terminator      633..673
                     /label=BBa_B0012
     terminator      545..673
                     /label=BBa_B0015
     CDS             830..1648
                     /label=kanamycin resistance
     misc_feature    682..1648
                     /label=BBa_P1003
     stem_loop       1668..1711
                     /label=stem_loop
     misc_feature    1657..1736
                     /label=BBa_B0010
     terminator      1657..1736
                     /label=BBa_B0010
     stem_loop       1752..1771
                     /label=T7 TE
     misc_feature    1778..1778
                     /label=stop
     polyA_site      1772..1785
                     /label=polya
     misc_feature    1745..1785
                     /label=BBa_B0012
     terminator      1745..1785
                     /label=BBa_B0012
     terminator      1657..1785
                     /label=BBa_B0015
     misc_feature    242..1785
                     /label=BBa_K329051
     misc_feature    1817..1822
                     /label=Coupling sequence
     misc_feature    1916..1930
                     /label=Left Xis binding site
     misc_feature    1794..2072
                     /label=BBa_K329046
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagttgaagt gtctacctat
       61 taaatattca aattttattg gaagtatctt tatatcttca cttttcaagg ataaatcgtc
      121 gtatcaaagc tcattcataa gtagtaaatt agtagtaaat tgagtggttt tgaccttgat
      181 aaagtgtgat aagtccagtt tttatgcgga taactagatt tttatgctat ttttactaga
      241 gtttacggct agctcagtcc taggtacaat gctagctact agagaaagag gagaaatact
      301 agatgaccat gattacggat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
      361 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
      421 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
      481 gctttgcctg gtttccggca ccagaagcgg tgccggaaag ctggctggag taataatact
      541 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
      601 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
      661 ttctgcgttt atatactaga gctgatcctt caactcagca aaagttcgat ttattcaaca
      721 aagccacgtt gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
      781 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat
      841 tcaacgggaa acgtcttgct cccgtccgcg cttaaactcc aacatggacg ctgatttata
      901 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta
      961 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga
     1021 tgttacagat gagatggtcc gtctcaactg gctgacggag tttatgcctc tcccgaccat
     1081 caagcatttt atccgtactc ctgatgatgc gtggttactc accaccgcga ttcctgggaa
     1141 aacagccttc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct
     1201 ggccgtgttc ctgcgccggt tacattcgat tcctgtttgt aattgtcctt ttaacagcga
     1261 tcgtgtattt cgtcttgctc aggcgcaatc acgcatgaat aacggtttgg ttgatgcgag
     1321 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcacaa
     1381 gctcttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct
     1441 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgggtcg gaatcgcaga
     1501 ccgttaccag gaccttgcca ttctttggaa ctgcctcggt gagttttctc cttcattaca
     1561 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca
     1621 tttgatgctc gatgagtttt tctaataata ctagagccag gcatcaaata aaacgaaagg
     1681 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     1741 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagactatga
     1801 agaaaaaaat gatttttaaa ataaacaaag tataaatttc taattatctt tttatatttt
     1861 cttaaatgct cgtaaagcct tattctatgt gctttcgagt atttttactg taggaagata
     1921 cttcacgttt ctttgcatat ttcctcatgt cttagctgtc agaagtggta aataagtagt
     1981 aaattcattt gtactactaa gcaacaagac gctcctgttg cttctcttta ttcaagcgtt
     2041 tcatttctgc cattgcagaa tcgaatgttg ca
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