LOCUS       BBa_K329060             1784 bp    DNA     linear       17-May-2024
DEFINITION  [KanR] in between MUT_MUT[Lambda] Tn916 Arms
ACCESSION   BBa_K329060
VERSION     BBa_K329060.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     misc_feature    172..361
                     /label=mutated right arm of tn916 
     misc_feature    44..171
                     /label=mutated attR lambda
     misc_feature    44..367
                     /label=BBa_K329043
     CDS             524..1342
                     /label=kanamycin resistance
     misc_feature    376..1342
                     /label=BBa_P1003
     stem_loop       1362..1405
                     /label=stem_loop
     misc_feature    1351..1430
                     /label=BBa_B0010
     terminator      1351..1430
                     /label=BBa_B0010
     stem_loop       1446..1465
                     /label=T7 TE
     misc_feature    1472..1472
                     /label=stop
     polyA_site      1466..1479
                     /label=polya
     misc_feature    1439..1479
                     /label=BBa_B0012
     terminator      1439..1479
                     /label=BBa_B0012
     terminator      1351..1479
                     /label=BBa_B0015
     misc_feature    1488..1737
                     /label=Left arm Tn916 MUT
     misc_feature    1738..1743
                     /label=Coupling
     misc_feature    1744..1751
                     /label=Mutation
     misc_feature    1752..1784
                     /label=Half of attL lambda MUT
     misc_feature    1488..1784
                     /label=BBa_K329048
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagctagaga ggtcactaat
       61 accatctaag tagttgattc atagtgactg catatgttgt gttttacagt attatgtagt
      121 ctgtttttta tgcaaaatct aatttaatat attgatacct tttttatttt aaaaatagca
      181 taaaaatcta gttatccgca taaaaactgg acttatcaca ctttatcaag gtcaaaacca
      241 ctcaatttac tactaattta ctacttatga atgagctttg atacgacgat ttatccttga
      301 aaagtgaaga tataaagata cttccaataa aatttgaata tttaataggt agacacttca
      361 atactagtac tagagctgat ccttcaactc agcaaaagtt cgatttattc aacaaagcca
      421 cgttgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac
      481 aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg
      541 ggaaacgtct tgctcccgtc cgcgcttaaa ctccaacatg gacgctgatt tatatgggta
      601 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa
      661 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac
      721 agatgagatg gtccgtctca actggctgac ggagtttatg cctctcccga ccatcaagca
      781 ttttatccgt actcctgatg atgcgtggtt actcaccacc gcgattcctg ggaaaacagc
      841 cttccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggccgt
      901 gttcctgcgc cggttacatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgtgt
      961 atttcgtctt gctcaggcgc aatcacgcat gaataacggt ttggttgatg cgagtgattt
     1021 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc acaagctctt
     1081 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt
     1141 tgacgagggg aaattaatag gttgtattga tgttggacgg gtcggaatcg cagaccgtta
     1201 ccaggacctt gccattcttt ggaactgcct cggtgagttt tctccttcat tacagaaacg
     1261 gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat
     1321 gctcgatgag tttttctaat aatactagag ccaggcatca aataaaacga aaggctcagt
     1381 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     1441 acactggctc accttcgggt gggcctttct gcgtttatat actagagtgc aacattcgat
     1501 tctgcaatgg cagaaatgaa acgcttgaat aaagagaagc aacaggagcg tcttgttgct
     1561 tagtagtaca aatgaattta ctacttattt accacttctg acagctaaat tccgctttgc
     1621 gactcaacct ttttcaccta aagtgcaccg accgtgaata ctcgaaattc tacccgtaga
     1681 atgcgtactg gtaatgcaaa ctaaataaaa agataattag aaatttatac tttgtttatt
     1741 ttaaaaatca tgttcagctt ttttatacta acttgagcga aacg
//