LOCUS       BBa_K336011             1975 bp    DNA     linear       13-May-2024
DEFINITION  p(tetR)+lasR+p(LasR)+mRFP1
ACCESSION   BBa_K336011
VERSION     BBa_K336011.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..803
                     /label=BBa_C0179
     stem_loop       823..866
                     /label=stem_loop
     misc_feature    812..891
                     /label=BBa_B0010
     terminator      812..891
                     /label=BBa_B0010
     stem_loop       907..926
                     /label=T7 TE
     misc_feature    933..933
                     /label=stop
     polyA_site      927..940
                     /label=polya
     misc_feature    900..940
                     /label=BBa_B0012
     terminator      900..940
                     /label=BBa_B0012
     misc_feature    994..1011
                     /label=OP2
     misc_feature    1054..1073
                     /label=OP1
     promoter        1065..1070
                     /label=-35
     promoter        1088..1093
                     /label=-10
     promoter        949..1105
                     /label=BBa_R0079
     RBS             1114..1126
                     /label=RBS-3\Weak
     RBS             1120..1123
                     /label=RBS
     misc_feature    1114..1126
                     /label=BBa_B0032
     RBS             1114..1126
                     /label=BBa_B0032
     CDS             1133..1807
                     /label=mrfp1
     misc_feature    1814..1838
                     /label=Help:Barcodes
     CDS             1133..1838
                     /label=BBa_E1010
     stem_loop       1858..1901
                     /label=stem_loop
     misc_feature    1847..1926
                     /label=BBa_B0010
     terminator      1847..1926
                     /label=BBa_B0010
     stem_loop       1942..1961
                     /label=T7 TE
     misc_feature    1968..1968
                     /label=stop
     polyA_site      1962..1975
                     /label=polya
     misc_feature    1935..1975
                     /label=BBa_B0012
     terminator      1935..1975
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggccttgg ttgacggttt tcttgagctg gaacgctcaa
      121 gtggaaaatt ggagtggagc gccatcctcc agaagatggc gagcgacctt ggattctcga
      181 agatcctgtt cggcctgttg cctaaggaca gccaggacta cgagaacgcc ttcatcgtcg
      241 gcaactaccc ggccgcctgg cgcgagcatt acgaccgggc tggctacgcg cgggtcgacc
      301 cgacggtcag tcactgtacc cagagcgtac tgccgatttt ctgggaaccg tccatctacc
      361 agacgcgaaa gcagcacgag ttcttcgagg aagcctcggc cgccggcctg gtgtatgggc
      421 tgaccatgcc gctgcatggt gctcgcggcg aactcggcgc gctgagcctc agcgtggaag
      481 cggaaaaccg ggccgaggcc aaccgtttca tagagtcggt cctgccgacc ctgtggatgc
      541 tcaaggacta cgcactgcaa agcggtgccg gactggcctt cgaacatccg gtcagcaaac
      601 cggtggttct gaccagccgg gagaaggaag tgttgcagtg gtgcgccatc ggcaagacca
      661 gttgggagat atcggttatc tgcaactgct cggaagccaa tgtgaacttc catatgggaa
      721 atattcggcg gaagttcggt gtgacctccc gccgcgtagc ggccattatg gccgttaatt
      781 tgggtcttat tactctctaa taatactaga gccaggcatc aaataaaacg aaaggctcag
      841 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
      901 cacactggct caccttcggg tgggcctttc tgcgtttata tactagaggc ccctcgctga
      961 gcgcgtcccg gagctggggg caacctagct gccacctgct tttctgctag ctattccagc
     1021 gaaaacatac agatttccgg cgaaatcaag gctacctgcc agttctggca ggtttggccg
     1081 cgggttcttt ttggtacacg aaagctacta gagtcacaca ggaaagtact agatggcttc
     1141 ctccgaagac gttatcaaag agttcatgcg tttcaaagtt cgtatggaag gttccgttaa
     1201 cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac
     1261 cgctaaactg aaagttacca aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc
     1321 gcagttccag tacggttcca aagcttacgt taaacacccg gctgacatcc cggactacct
     1381 gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg
     1441 tgttgttacc gttacccagg actcctccct gcaagacggt gagttcatct acaaagttaa
     1501 actgcgtggt accaacttcc cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg
     1561 ggaagcttcc accgaacgta tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat
     1621 gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca cctacatggc
     1681 taaaaaaccg gttcagctgc cgggtgctta caaaaccgac atcaaactgg acatcacctc
     1741 ccacaacgaa gactacacca tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac
     1801 cggtgcttaa taacgctgat agtgctagtg tagatcgcta ctagagccag gcatcaaata
     1861 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     1921 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//