LOCUS       BBa_K336016              524 bp    DNA     linear       25-May-2024
DEFINITION  Plux+RBS+ccdB+stop
ACCESSION   BBa_K336016
VERSION     BBa_K336016.1  
FEATURES             Location/Qualifiers
     misc_feature    20..25
                     /label=-35 Region
     misc_feature    42..47
                     /label=-10 Region
     misc_feature    1..20
                     /label=LuxR 
     promoter        1..55
                     /label=BBa_K091156
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    82..84
                     /label=start
     misc_feature    385..387
                     /label=stop
     CDS             82..384
                     /label=cds
     CDS             82..387
                     /label=BBa_K145151
     stem_loop       407..450
                     /label=stem_loop
     misc_feature    396..475
                     /label=BBa_B0010
     terminator      396..475
                     /label=BBa_B0010
     stem_loop       491..510
                     /label=T7 TE
     misc_feature    517..517
                     /label=stop
     polyA_site      511..524
                     /label=polya
     misc_feature    484..524
                     /label=BBa_B0012
     terminator      484..524
                     /label=BBa_B0012
ORIGIN
        1 acctgtagga tcgtacaggt ttaatcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagaaagagg agaaatacta gatgcagttt aaggtttaca cctataaaag agagagccgt
      121 tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg acggatggtg
      181 atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg
      241 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc
      301 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
      361 attaacctga tgttctgggg aatataatac tagagccagg catcaaataa aacgaaaggc
      421 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
      481 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//