LOCUS       BBa_K336018             1866 bp    DNA     linear       18-May-2024
DEFINITION  p(tetR),LuxI and LuxR
ACCESSION   BBa_K336018
VERSION     BBa_K336018.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    660..691
                     /label=LVA
     CDS             81..659
                     /label=luxI
     misc_feature    81..698
                     /label=BBa_C0061
     misc_feature    699..723
                     /label=Help:Barcodes
     CDS             81..698
                     /label=BBa_C0061
     stem_loop       743..786
                     /label=stem_loop
     misc_feature    732..811
                     /label=BBa_B0010
     terminator      732..811
                     /label=BBa_B0010
     stem_loop       827..846
                     /label=T7 TE
     misc_feature    853..853
                     /label=stop
     polyA_site      847..860
                     /label=polya
     misc_feature    820..860
                     /label=BBa_B0012
     terminator      820..860
                     /label=BBa_B0012
     misc_feature    869..887
                     /label=TetR 1
     misc_feature    869..922
                     /label=BBa_R0040
     promoter        888..893
                     /label=-35
     misc_feature    894..912
                     /label=TetR 2
     promoter        911..916
                     /label=-10
     promoter        869..922
                     /label=BBa_R0040
     misc_feature    935..938
                     /label=conserved
     RBS             931..942
                     /label=BBa_B0034
     misc_feature    949..950
                     /label=prefix
     misc_feature    1122..1122
                     /label=T
     misc_feature    1440..1440
                     /label=A
     CDS             949..1698
                     /label=luxR
     misc_feature    949..1704
                     /label=BBa_C0062
     misc_feature    1705..1729
                     /label=Help:Barcodes
     CDS             949..1704
                     /label=BBa_C0062
     stem_loop       1749..1792
                     /label=stem_loop
     misc_feature    1738..1817
                     /label=BBa_B0010
     terminator      1738..1817
                     /label=BBa_B0010
     stem_loop       1833..1852
                     /label=T7 TE
     misc_feature    1859..1859
                     /label=stop
     polyA_site      1853..1866
                     /label=polya
     misc_feature    1826..1866
                     /label=BBa_B0012
     terminator      1826..1866
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgactataa tgataaaaaa atcggatttt ttggcaattc
      121 catcggagga gtataaaggt attctaagtc ttcgttatca agtgtttaag caaagacttg
      181 agtgggactt agttgtagaa aataaccttg aatcagatga gtatgataac tcaaatgcag
      241 aatatattta tgcttgtgat gatactgaaa atgtaagtgg atgctggcgt ttattaccta
      301 caacaggtga ttatatgctg aaaagtgttt ttcctgaatt gcttggtcaa cagagtgctc
      361 ccaaagatcc taatatagtc gaattaagtc gttttgctgt aggtaaaaat agctcaaaga
      421 taaataactc tgctagtgaa attacaatga aactatttga agctatatat aaacacgctg
      481 ttagtcaagg tattacagaa tatgtaacag taacatcaac agcaatagag cgatttttaa
      541 agcgtattaa agttccttgt catcgtattg gagacaaaga aattcatgta ttaggtgata
      601 ctaaatcggt tgtattgtct atgcctatta atgaacagtt taaaaaagca gtcttaaatg
      661 ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc tagtgtagat
      721 ctctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
      781 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
      841 tgggcctttc tgcgtttata tactagagtc cctatcagtg atagagattg acatccctat
      901 cagtgataga gatactgagc actactagag aaagaggaga aatactagat gaaaaacata
      961 aatgccgacg acacatacag aataattaat aaaattaaag cttgtagaag caataatgat
     1021 attaatcaat gcttatctga tatgactaaa atggtacatt gtgaatatta tttactcgcg
     1081 atcatttatc ctcattctat ggttaaatct gatatttcaa tcctagataa ttaccctaaa
     1141 aaatggaggc aatattatga tgacgctaat ttaataaaat atgatcctat agtagattat
     1201 tctaactcca atcattcacc aattaattgg aatatatttg aaaacaatgc tgtaaataaa
     1261 aaatctccaa atgtaattaa agaagcgaaa acatcaggtc ttatcactgg gtttagtttc
     1321 cctattcata cggctaacaa tggcttcgga atgcttagtt ttgcacattc agaaaaagac
     1381 aactatatag atagtttatt tttacatgcg tgtatgaaca taccattaat tgttccttct
     1441 ctagttgata attatcgaaa aataaatata gcaaataata aatcaaacaa cgatttaacc
     1501 aaaagagaaa aagaatgttt agcgtgggca tgcgaaggaa aaagctcttg ggatatttca
     1561 aaaatattag gttgcagtga gcgtactgtc actttccatt taaccaatgc gcaaatgaaa
     1621 ctcaatacaa caaaccgctg ccaaagtatt tctaaagcaa ttttaacagg agcaattgat
     1681 tgcccatact ttaaaaatta ataacactga tagtgctagt gtagatcact actagagcca
     1741 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     1801 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     1861 tttata
//