BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_K336047
1
BBa_K336047
promoter+RBS+SBFP2+double termnator
2010-10-25T11:00:00Z
2015-05-08T01:12:05Z
iGEM parts
This is the device that express SBFP2. It???s made up with BBa_J23110, BBa_I156010, BBa_B0010 and BBa_B0012.
false
false
_458_
0
5965
9
Not in stock
false
design
false
Ryuto Nitta
component2103884
1
BBa_J23110
component2103886
1
BBa_B0010
component2103885
1
BBa_K156010
component2103888
1
BBa_B0012
annotation2103885
1
BBa_K156010
range2103885
1
42
761
annotation2103886
1
BBa_B0010
range2103886
1
770
849
annotation2103888
1
BBa_B0012
range2103888
1
858
898
annotation2103884
1
BBa_J23110
range2103884
1
1
35
BBa_J23110
1
BBa_J23110
constitutive promoter family member
2006-08-16T11:00:00Z
2015-08-31T04:08:40Z
Later
Later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_K156010
1
SBFP2
SBFP2 (strongly enhanced blue fluorescent protein)
2008-10-28T12:00:00Z
2016-01-26T09:51:52Z
OFP was cloned from the tentacles of ''Cerianthus'' sp., a tube anemone. The original sequence was obtained from GenBank (VERSION AY296063.1, GI:31616578).
This part codes for orange fluorescent protein (OFP). Fluorescent proteins are useful tools for visualizing and quantifying protein expression in cells. OFP is yet another fluorescent protein, like GFP, that can be utilized as a reporter.
false
false
_246_
4206
1475
9
It's complicated
true
This part was codon-optimized for expression in ''E. coli''. The following restriction enzyme sites were also removed: EcoRI, NotI, XbaI, SpeI, PstI, NheI, AvrII, ApoI, MfeI, NsiI, SbfI.
false
George McArthur IV
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K336047_sequence
1
tttacggctagctcagtcctaggtacaatgctagctactagatggttagcaaaggtgaagagctgtttactggtgttgttccgatcctggttgaactggacggtgatgttaacggccacaagttctccgtgtctggcgaaggcgagggcgatgctacctacggtaaactgaccctgaagttcatctgtaccactggtaaactgccggtaccgtggccgaccctggttaccaccctgagccatggtgttcagtgttttgcacgttacccggatcacatgaagcagcatgatttcttcaagtctgcgatgccggagggttacgtccaggaacgcactatcttcttcaaagacgacggcaactataaaacccgtgccgaggttaagtttgagggtgacaccctggtcaaccgtattgaactgaaaggtatcgacttcaaagaagacggcaacattctgggtcacaaactggagtacaacttcaacagccataacgtgtacatcaccgccgataaacagaagaacggcatcaaagctaacttcaagattcgtcacaacatcgaagatggtggtgttcaactggctgatcactaccagcagaacacgccgatcggtgatggcccggtactgctgcctgacaaccattatctgtctacccagtctaaactgagcaaagacccgaatgagaaacgtgatcacatggtgctgctggagttcgtgactgcggcaggtatcacgctgggtatggacgaactgtataaatgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K156010_sequence
1
atggttagcaaaggtgaagagctgtttactggtgttgttccgatcctggttgaactggacggtgatgttaacggccacaagttctccgtgtctggcgaaggcgagggcgatgctacctacggtaaactgaccctgaagttcatctgtaccactggtaaactgccggtaccgtggccgaccctggttaccaccctgagccatggtgttcagtgttttgcacgttacccggatcacatgaagcagcatgatttcttcaagtctgcgatgccggagggttacgtccaggaacgcactatcttcttcaaagacgacggcaactataaaacccgtgccgaggttaagtttgagggtgacaccctggtcaaccgtattgaactgaaaggtatcgacttcaaagaagacggcaacattctgggtcacaaactggagtacaacttcaacagccataacgtgtacatcaccgccgataaacagaagaacggcatcaaagctaacttcaagattcgtcacaacatcgaagatggtggtgttcaactggctgatcactaccagcagaacacgccgatcggtgatggcccggtactgctgcctgacaaccattatctgtctacccagtctaaactgagcaaagacccgaatgagaaacgtgatcacatggtgctgctggagttcgtgactgcggcaggtatcacgctgggtatggacgaactgtataaatga
BBa_J23110_sequence
1
tttacggctagctcagtcctaggtacaatgctagc
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z