LOCUS BBa_K337035 5234 bp DNA linear 12-Jun-2024 DEFINITION pSMB_miTuner Plasmid HD2 (BGH(rc)/shRNA10(rc)/sv40(rc)/CMV/Luc2_sv4 0/CMV/Kozag_hRluc_BGH) ACCESSION BBa_K337035 VERSION BBa_K337035.1 FEATURES Location/Qualifiers misc_feature 1..255 /label=BGH Terminator promoter 427..846 /label=SV40 misc_feature 261..421 /label=synthetic microRNA promoter 852..1461 /label=CMV CDS 1467..3416 /label=Luc2 promoter 3422..4031 /label=CMV CDS 4037..4979 /label=hRluc misc_feature 4985..5234 /label=BGH Terminator ORIGIN 1 ggatccccat agagcccacc gcatccccag catgcctgct attgtcttcc caatcctccc 61 ccttgctgtc ctgccccacc ccacccccca gaatagaatg acacctactc agacaatgcg 121 atgcaatttc ctcattttat taggaaagga cagtgggagt ggcaccttcc agggtcaagg 181 aaggcacggg ggaggggcaa acaacagatg gctggcaact agaaggcaca gtcgaggctg 241 atcagcgggt ttaaagctag taagcaaacg atgccaagac atttatcgag ggaaggattg 301 cctagcagta gctaggtacc acggtcaccg atattaatag tttagacata atatcggtgg 361 ccgtggtgtc cagctctgct aaggaaactc tgtagccacg aaggtgttaa cttcacctcc 421 agctagtaag ctttttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga 481 atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat 541 ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg 601 ggactatggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc 661 ctggggactt tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc 721 ctgctgggga gcctggggac tttccacacc ctaactgaca cacattccac agctggttct 781 ttccgcctca gaaggtacct aaccaagttc ctctttcaga ggttatttca ggccatggtg 841 ctgcgcgcta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg 901 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc 961 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat 1021 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat 1081 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat 1141 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc 1201 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac 1261 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa 1321 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt 1381 aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact 1441 gcttactggc ttatcgaaat ggctagtgcc accatggaag atgccaaaaa cattaagaag 1501 ggcccagcgc cattctaccc actcgaagac gggaccgccg gcgagcagct gcacaaagcc 1561 atgaagcgct acgccctggt gcccggcacc atcgccttta ccgacgcaca tatcgaggtg 1621 gacattacct acgccgagta cttcgagatg agcgttcggc tggcagaagc tatgaagcgc 1681 tatgggctga atacaaacca tcggatcgtg gtgtgcagcg agaatagctt gcagttcttc 1741 atgcccgtgt tgggtgccct gttcatcggt gtggctgtgg ccccagctaa cgacatctac 1801 aacgagcgcg agctgctgaa cagcatgggc atcagccagc ccaccgtcgt attcgtgagc 1861 aagaaagggc tgcaaaagat cctcaacgtg caaaagaagc taccgatcat acaaaagatc 1921 atcatcatgg atagcaagac cgactaccag ggcttccaaa gcatgtacac cttcgtgact 1981 tcccatttgc cacccggctt caacgagtac gacttcgtgc ccgagagctt cgaccgggac 2041 aaaaccatcg ccctgatcat gaacagtagt ggcagtaccg gattgcccaa gggcgtagcc 2101 ctaccgcacc gcaccgcttg tgtccgattc agtcatgccc gcgaccccat cttcggcaac 2161 cagatcatcc ccgacaccgc tatcctcagc gtggtgccat ttcaccacgg cttcggcatg 2221 ttcaccacgc tgggctactt gatctgcggc tttcgggtcg tgctcatgta ccgcttcgag 2281 gaggagctat tcttgcgcag cttgcaagac tataagattc aatctgccct gctggtgccc 2341 acactattta gcttcttcgc taagagcact ctcatcgaca agtacgacct aagcaacttg 2401 cacgagatcg ccagcggcgg ggcgccgctc agcaaggagg taggtgaggc cgtggccaaa 2461 cgcttccacc taccaggcat ccgccagggc tacggcctga cagaaacaac cagcgccatt 2521 ctgatcaccc ccgaagggga cgacaagcct ggcgcagtag gcaaggtggt gcccttcttc 2581 gaggctaagg tggtggactt ggacaccggt aagacactgg gtgtgaacca gcgcggcgag 2641 ctgtgcgtcc gtggccccat gatcatgagc ggctacgtta acaaccccga ggctacaaac 2701 gctctcatcg acaaggacgg ctggctgcac agcggcgaca tcgcctactg ggacgaggac 2761 gagcacttct tcatcgtgga ccggctgaag agcctgatca aatacaaggg ctaccaggta 2821 gccccagccg aactggagag catcctgctg caacacccca acatcttcga cgccggggtc 2881 gccggcctgc ccgacgacga tgccggcgag ctgcccgccg cagtcgtcgt gctggaacac 2941 ggtaaaacca tgaccgagaa ggagatcgtg gactatgtgg ccagccaggt tacaaccgcc 3001 aagaagctgc gcggtggtgt tgtgttcgtg gacgaggtgc ctaaaggact gaccggcaag 3061 ttggacgccc gcaagatccg cgagattctc attaaggcca agaagggcgg caagatcgcc 3121 gtgtaataat tctagccccg gggcggtcag aactcgaggc tagagtcggg gcggccggcc 3181 gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca 3241 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat 3301 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg 3361 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta ggatccgcta 3421 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 3481 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 3541 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 3601 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 3661 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 3721 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 3781 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat 3841 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 3901 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac 3961 ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact gcttactggc 4021 ttatcgaaat ggctagtgcc accatggctt ccaaggtgta cgaccccgag caacgcaaac 4081 gcatgatcac tgggcctcag tggtgggctc gctgcaagca aatgaacgtg ctggactcct 4141 tcatcaacta ctatgattcc gagaagcacg ccgagaacgc cgtgattttt ctgcatggta 4201 acgctgcctc cagctacctg tggaggcacg tcgtgcctca catcgagccc gtggctagat 4261 gcatcatccc tgatctgatc ggaatgggta agtccggcaa gagcgggaat ggctcatatc 4321 gcctcctgga tcactacaag tacctcaccg cttggttcga gctgctgaac cttccaaaga 4381 aaatcatctt tgtgggccac gactgggggg cttgtctggc ctttcactac tcctacgagc 4441 accaagacaa gatcaaggcc atcgtccatg ctgagagtgt cgtggacgtg atcgagtcct 4501 gggacgagtg gcctgacatc gaggaggata tcgccctgat caagagcgaa gagggcgaga 4561 aaatggtgct tgagaataac ttcttcgtcg agaccatgct cccaagcaag atcatgcgga 4621 aactggagcc tgaggagttc gctgcctacc tggagccatt caaggagaag ggcgaggtta 4681 gacggcctac cctctcctgg cctcgcgaga tccctctcgt taagggaggc aagcccgacg 4741 tcgtccagat tgtccgcaac tacaacgcct accttcgggc cagcgacgat ctgcctaaga 4801 tgttcatcga gtccgaccct gggttctttt ccaacgctat tgtcgaggga gctaagaagt 4861 tccctaacac cgagttcgtg aaggtgaagg gcctccactt cagccaggag gacgctccag 4921 atgaaatggg taagtacatc aagagcttcg tggagcgcgt gctgaagaac gagcagtaag 4981 ctagttttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt 5041 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta 5101 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg 5161 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc 5221 ggtgggctct atgg //