LOCUS       BBa_K338062             1064 bp    DNA     linear       29-May-2024
DEFINITION  GFP Reporter Device, Heat-Shock or IPTG Induced
ACCESSION   BBa_K338062
VERSION     BBa_K338062.1  
FEATURES             Location/Qualifiers
     promoter        1..98
                     /label=BBa_K338001
     misc_feature    109..125
                     /label=lac O1
     promoter        126..131
                     /label=-35
     misc_feature    132..148
                     /label=lac O1
     promoter        149..154
                     /label=-10
     misc_feature    107..160
                     /label=BBa_R0011
     promoter        107..160
                     /label=BBa_R0011
     misc_feature    174..177
                     /label=conserved
     RBS             170..181
                     /label=BBa_B0034
     promoter        1..181
                     /label=BBa_K338002
     misc_feature    194..197
                     /label=conserved
     RBS             190..201
                     /label=BBa_B0034
     CDS             208..927
                     /label=GFP protein
     CDS             208..927
                     /label=BBa_E0040
     stem_loop       947..990
                     /label=stem_loop
     misc_feature    936..1015
                     /label=BBa_B0010
     terminator      936..1015
                     /label=BBa_B0010
     stem_loop       1031..1050
                     /label=T7 TE
     misc_feature    1057..1057
                     /label=stop
     polyA_site      1051..1064
                     /label=polya
     misc_feature    1024..1064
                     /label=BBa_B0012
     terminator      1024..1064
                     /label=BBa_B0012
     terminator      936..1064
                     /label=BBa_B0015
ORIGIN
        1 ccgaggtcct tgttgcgaag attgatgaca atgtgagtgc ttcccttgaa accctgaaac
       61 tgatccccat aataagcgaa gttagcgaga tgaatgcgta ctagagaatt gtgagcggat
      121 aacaattgac attgtgagcg gataacaaga tactgagcac atactagaga aagaggagaa
      181 atactagaga aagaggagaa atactagatg cgtaaaggag aagaactttt cactggagtt
      241 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga
      301 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga
      361 aaactacctg ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt
      421 gcgagatacc cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt
      481 tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa
      541 gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa
      601 gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac
      661 atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt
      721 gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
      781 cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc
      841 aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat
      901 ggcatggatg aactatacaa ataataatac tagagccagg catcaaataa aacgaaaggc
      961 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     1021 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
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