LOCUS       BBa_K341456             1286 bp    DNA     linear       01-Jun-2024
DEFINITION  Kanamycin resistance(with Promoter) + I-sceI site+ Recombination 
            site
ACCESSION   BBa_K341456
VERSION     BBa_K341456.1  
FEATURES             Location/Qualifiers
     CDS             68..862
                     /label=Km Resistance gene
     misc_feature    33..57
                     /label=Recombination site
     misc_feature    1230..1254
                     /label=Recombination site
     misc_feature    1273..1281
                     /label=Dra III cutting site
     misc_feature    6..14
                     /label=Dra III cutting site
     misc_feature    15..32
                     /label=I-sceI cutting site
     misc_feature    1255..1272
                     /label=I-sceI cutting site
     promoter        863..1229
                     /label=promoter
ORIGIN
        1 attatcacgg agtgtaggga taacagggta atgatggcgc ctcatccctg aagccaagat
       61 ccccttatta gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag
      121 cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa
      181 tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt
      241 cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat
      301 gggtcacgac gagatcctcg ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg
      361 ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca
      421 tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg
      481 gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag
      541 caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc
      601 ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg
      661 atagccgcgc tgcctcgtcc tgtagttcat tcagggcacc ggacaggtcg gtcttgacaa
      721 aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg
      781 tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt
      841 gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga tcagatcttg
      901 atcccctgcg ccatcagatc cttggcggca agaaagccat ccagtttact ttgcagggct
      961 tcccaacctt accagagggc gccccagctg gcaattccgg ttcgcttgct gtccataaaa
     1021 ccgcccagtc tagctatcgc catgtaagcc cactgcaagc tacctgcttt ctctttgcgc
     1081 ttgcgttttc ccttgtccag atagcccagt agctgacatt catccggggt cagcaccgtt
     1141 tctgcggact ggctttctac gtgttccgct tcctttagca gcccttgcgc cctgagtgct
     1201 tgcggcagcg tgagcttcaa aagcgctctt caccgtttgg accttggggc cgtaattacc
     1261 ctgttatccc tacaccaggt gataat
//