LOCUS       BBa_K343004             1149 bp    DNA     linear       19-May-2024
DEFINITION  Flagella overekspression
ACCESSION   BBa_K343004
VERSION     BBa_K343004.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     promoter        1..74
                     /label=BBa_J13002
     misc_feature    81..83
                     /label=Start codon
     CDS             81..429
                     /label=FlhD
     misc_feature    430..432
                     /label=Stop codon
     misc_feature    436..438
                     /label=Start codon 
     CDS             439..1009
                     /label=FlhC
     misc_feature    1010..1012
                     /label=Stop codon
     misc_feature    902..902
                     /label=Changed a T to C to remove Pst1 restriction site.
     CDS             81..1012
                     /label=BBa_K343000
     stem_loop       1032..1075
                     /label=stem_loop
     misc_feature    1021..1100
                     /label=BBa_B0010
     terminator      1021..1100
                     /label=BBa_B0010
     stem_loop       1116..1135
                     /label=T7 TE
     misc_feature    1142..1142
                     /label=stop
     polyA_site      1136..1149
                     /label=polya
     misc_feature    1109..1149
                     /label=BBa_B0012
     terminator      1109..1149
                     /label=BBa_B0012
     terminator      1021..1149
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgcatacct ccgagttgct gaaacacatt tatgacatca
      121 acttgtcata tttactactt gcacagcgtt tgattgttca ggacaaagcg tccgctatgt
      181 ttcgtctcgg cataaatgaa gaaatggcga caacgttagc ggcactgact cttccgcaaa
      241 tggttaagct ggcagaaacc aatcaactgg tttgtcactt ccgttttgac agccaccaga
      301 cgattactca gttgacgcaa gattcccgcg ttgacgatct ccagcaaatt cataccggca
      361 tcatgctctc aacacgcttg ctgaatgatg ttaatcagcc tgaagaagcg ctgcgcaaga
      421 aaagggcctg atcatgagtg aaaaaagcat tgttcaggaa gcgcgggata ttcagctggc
      481 aatggaattg atcaccctgg gcgctcgttt gcagatgctg gaaagcgaaa cacagttaag
      541 tcgcggacgc ctgataaaac tttataaaga actgcgcgga agcccaccgc cgaaaggcat
      601 gctgccattc tcaaccgact ggtttatgac ctgggaacaa aacgttcatg cttcgatgtt
      661 ctgtaatgca tggcagtttt tactgaaaac cggtttgtgt aatggcgtcg atgcggtgat
      721 caaagcctac cgtttatacc ttgaacagtg cccacaagca gaagaaggac cactgctggc
      781 attaacccgt gcctggacat tggtgcggtt tgttgaaagt ggattactgc aactttccag
      841 ctgcaactgc tgcggcggca attttattac ccacgctcac cagcctgttg gcagctttgc
      901 ccgcagctta tgtcaaccgc catcccgggc agtaaaaaga cgtaaacttt cccagaatcc
      961 tgccgatatt atcccacaac tgctggatga acagagagta caggctgttt aatactagag
     1021 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1081 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     1141 gcgtttata
//