LOCUS       BBa_K346025              943 bp    DNA     linear       01-Jun-2024
DEFINITION  PmerT promoter mutant 88+RBS(B0030)+GFP(E0040)Terminator(B0010)+Ter
            minator(B0012)
ACCESSION   BBa_K346025
VERSION     BBa_K346025.1  
FEATURES             Location/Qualifiers
     promoter        1..57
                     /label=BBa_K346060
     RBS             66..80
                     /label=RBS-1\Strong
     RBS             73..77
                     /label=RBS
     misc_feature    66..80
                     /label=BBa_B0030
     RBS             66..80
                     /label=BBa_B0030
     CDS             87..806
                     /label=GFP protein
     CDS             87..806
                     /label=BBa_E0040
     stem_loop       826..869
                     /label=stem_loop
     misc_feature    815..894
                     /label=BBa_B0010
     terminator      815..894
                     /label=BBa_B0010
     stem_loop       910..929
                     /label=T7 TE
     misc_feature    936..936
                     /label=stop
     polyA_site      930..943
                     /label=polya
     misc_feature    903..943
                     /label=BBa_B0012
     terminator      903..943
                     /label=BBa_B0012
     misc_feature    66..943
                     /label=BBa_E0840
ORIGIN
        1 ttccatatcg cttgacgccg tacatgagta cggaagtaag gttacgctat ccaatcctac
       61 tagagattaa agaggagaaa tactagatgc gtaaaggaga agaacttttc actggagttg
      121 tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag
      181 agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa
      241 aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg
      301 cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt
      361 atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag
      421 tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag
      481 aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca
      541 tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg
      601 aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc
      661 ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca
      721 acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg
      781 gcatggatga actatacaaa taataatact agagccaggc atcaaataaa acgaaaggct
      841 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      901 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//