BBa_K357000 1 BBa_K357000 catechol 1 2-dioxygenase 2010-06-22T11:00:00Z 2015-05-08T01:12:11Z This part is designed from genomic sequence: AUTHORS Nelson,K., Paulsen,I., Weinel,C., Dodson,R., Hilbert,H., Fouts,D., Gill,S., Pop,M., Martins Dos Santos,V., Holmes,M., Brinkac,L., Beanan,M., DeBoy,R., Daugherty,S., Kolonay,J., Madupu,R., Nelson,W., White,O., Peterson,J., Khouri,H., Hance,I., Lee,P., Holtzapple,E., Scanlan,D., Tran,K., Moazzez,A., Utterback,T., Rizzo,M., Lee,K., Kosack,D., Moestl,D., Wedler,H., Lauber,J., Hoheisel,J., Straetz,M., Heim,S., Kiewitz,C., Eisen,J., Timmis,K., Duesterhoft,A., Tummler,B. and Fraser,C. TITLE Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440 JOURNAL Environ. Microbiol. 4 (12), 799-808 (2002) Catechol dioxygenases are metalloprotein enzymes that carry out the oxidative cleavage of catechols. This class of enzymes incorporate dioxygen into the substrate. There are several family members in the Catechol dioxygenases family with different specificities, these include; catechol 1,2-dioxygenase (EC 1.13.11.1), catechol 2,3-dioxygenase (EC 1.13.11.2), and protocatechuate 3,4-dioxygenase (EC 1.13.11.3). The active site of catechol dioxygenases most frequently contains iron, but manganese-containing forms are also known. Catechol 1 2-dioxygenase utilizes Iron at its active site and it converts catechol into cis,cis-muconic acid. false false _530_ 0 5655 9 Not in stock true The sequence is was changed from the original to remove a PstI restriction site located around bp 263, to remove the restriction site while maintaining a conserved aa sequence. false Anish Kapadia annotation2071483 1 Removed EcoR1 Restriction Site range2071483 1 514 519 annotation2071482 1 Start Codon range2071482 1 1 3 annotation2071495 1 Stop Codon range2071495 1 934 936 BBa_K357000_sequence 1 atgaccgtgaaaatcagccataccgccgatatccaagcattctttaaccgtgttgccggactggaccatgccgaaggaaatcctcgcttcaaacaaatcatcctgcgtgttctgcaagacacagcacgtctgatcgaagacctggagatcacagaggacgaattttggcacgccgtggattatctgaatcgcctgggtggccgtaatgaggccggtctgctggcagctggtctgggtattgagcactttctggacctgctgcaagatgccaaagatgccgaggcaggactgggtggtggtacacctcgtacgatcgagggtccactgtatgttgctggtgctccactggcacaaggtgaagctcgtatggacgatggtactgatcctggagttgtcatgtttctgcaaggccaggtttttgatgccgacggtaaaccactggcaggggctactgttgacctgtggcacgctaatacacaaggcacctattcctatttcgactcgacacaatccgagttcaatctgcgtcgccgtatcatcacagatgccgaaggtcgttatcgtgctcgttccattgttccgagcggttatggttgtgacccacaaggaccgacacaagagtgtctggatctgctgggtcgtcatggacaacgccctgctcatgttcactttttcatcagcgccccaggacaccgtcatctgactactcagatcaactttgctggcgacaaatatctgtgggatgacttcgcctatgctacccgtgatggcctgatcggtgaactgcgttttgtggaggatgctgccgctgctcgtgaccgtggtgttcaaggagaacgtttcgccgaactgtcattcgacttccgcctgcaaggggcaaaatcacctgatgctgaagcacgttcacaccgccctcgtgcactgcaagaaggataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z