LOCUS       BBa_K360024             1871 bp    DNA     linear       21-May-2024
DEFINITION  RBS + cI  inverter + GFP
ACCESSION   BBa_K360024
VERSION     BBa_K360024.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             22..729
                     /label=cI lambda
     misc_feature    730..762
                     /label=LVA
     misc_feature    769..793
                     /label=Help:Barcodes
     CDS             19..768
                     /label=BBa_C0051
     stem_loop       813..856
                     /label=stem_loop
     misc_feature    802..881
                     /label=BBa_B0010
     terminator      802..881
                     /label=BBa_B0010
     stem_loop       897..916
                     /label=T7 TE
     misc_feature    923..923
                     /label=stop
     polyA_site      917..930
                     /label=polya
     misc_feature    890..930
                     /label=BBa_B0012
     terminator      890..930
                     /label=BBa_B0012
     misc_feature    1..930
                     /label=BBa_P0451
     promoter        976..981
                     /label=-10
     promoter        953..958
                     /label=-35
     misc_feature    963..979
                     /label=OR1
     misc_feature    939..955
                     /label=OR2
     misc_feature    939..987
                     /label=BBa_R0051
     promoter        939..987
                     /label=BBa_R0051
     RBS             996..1008
                     /label=RBS-3\Weak
     RBS             1002..1005
                     /label=RBS
     misc_feature    996..1008
                     /label=BBa_B0032
     RBS             996..1008
                     /label=BBa_B0032
     CDS             1015..1734
                     /label=GFP protein
     CDS             1015..1734
                     /label=BBa_E0040
     stem_loop       1754..1797
                     /label=stem_loop
     misc_feature    1743..1822
                     /label=BBa_B0010
     terminator      1743..1822
                     /label=BBa_B0010
     stem_loop       1838..1857
                     /label=T7 TE
     misc_feature    1864..1864
                     /label=stop
     polyA_site      1858..1871
                     /label=polya
     misc_feature    1831..1871
                     /label=BBa_B0012
     terminator      1831..1871
                     /label=BBa_B0012
     misc_feature    939..1871
                     /label=BBa_K098991
ORIGIN
        1 aaagaggaga aatactagat gagcacaaaa aagaaaccat taacacaaga gcagcttgag
       61 gacgcacgtc gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg cttatcccag
      121 gaatctgtcg cagacaagat ggggatgggg cagtcaggcg ttggtgcttt atttaatggc
      181 atcaatgcat taaatgctta taacgccgca ttgcttgcaa aaattctcaa agttagcgtt
      241 gaagaattta gcccttcaat cgccagagaa atctacgaga tgtatgaagc ggttagtatg
      301 cagccgtcac ttagaagtga gtatgagtac cctgtttttt ctcatgttca ggcagggatg
      361 ttctcacctg agcttagaac ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc
      421 aaaaaagcca gtgattctgc attctggctt gaggttgaag gtaattccat gaccgcacca
      481 acaggctcca agccaagctt tcctgacgga atgttaattc tcgttgaccc tgagcaggct
      541 gttgagccag gtgatttctg catagccaga cttgggggtg atgagtttac cttcaagaaa
      601 ctgatcaggg atagcggtca ggtgttttta caaccactaa acccacagta cccaatgatc
      661 ccatgcaatg agagttgttc cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag
      721 acgtttggcg ctgcaaacga cgaaaactac gctttagtag cttaataacg ctgatagtgc
      781 tagtgtagat cgctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
      841 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
      901 caccttcggg tgggcctttc tgcgtttata tactagagta acaccgtgcg tgttgactat
      961 tttacctctg gcggtgataa tggttgctac tagagtcaca caggaaagta ctagatgcgt
     1021 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
     1081 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt
     1141 acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact
     1201 actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac
     1261 tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat
     1321 gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga
     1381 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa
     1441 tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa
     1501 gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat
     1561 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc
     1621 acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag
     1681 tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata ataatactag
     1741 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     1801 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     1861 ctgcgtttat a
//