LOCUS       BBa_K360124              933 bp    DNA     linear       23-May-2024
DEFINITION  trpL promoter + GFP
ACCESSION   BBa_K360124
VERSION     BBa_K360124.1  
FEATURES             Location/Qualifiers
     misc_feature    29..45
                     /label=trpR binding site -3.5
     misc_feature    21..40
                     /label=trpR binding site  -11.5
     misc_feature    13..30
                     /label=trpR binding site  -19.5
     promoter        3..11
                     /label=-35 
     promoter        28..35
                     /label=-10
     promoter        1..49
                     /label=BBa_K360023
     RBS             58..70
                     /label=RBS-3\Weak
     RBS             64..67
                     /label=RBS
     misc_feature    58..70
                     /label=BBa_B0032
     RBS             58..70
                     /label=BBa_B0032
     CDS             77..796
                     /label=GFP protein
     CDS             77..796
                     /label=BBa_E0040
     stem_loop       816..859
                     /label=stem_loop
     misc_feature    805..884
                     /label=BBa_B0010
     terminator      805..884
                     /label=BBa_B0010
     stem_loop       900..919
                     /label=T7 TE
     misc_feature    926..926
                     /label=stop
     polyA_site      920..933
                     /label=polya
     misc_feature    893..933
                     /label=BBa_B0012
     terminator      893..933
                     /label=BBa_B0012
     misc_feature    58..933
                     /label=BBa_E0240
ORIGIN
        1 gctgttgaca attaatcatc gaactagtta actagtacgc aagttcacgt actagagtca
       61 cacaggaaag tactagatgc gtaaaggaga agaacttttc actggagttg tcccaattct
      121 tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg
      181 tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt
      241 tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc
      301 agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga
      361 aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga
      421 aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa
      481 cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga
      541 caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag
      601 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt
      661 accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag
      721 agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga
      781 actatacaaa taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
      841 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
      901 gctcaccttc gggtgggcct ttctgcgttt ata
//