BBa_K360141
1
BBa_K360141
Min. Blue Promoter + RBS + GFP BBa_E0040
2010-10-21T11:00:00Z
2015-05-08T01:12:12Z
The Minimum Blue Light Receptor Promoter was synthesized in a primer to join it with any plasmid in BioBrick format.
The Strong RBS and spacer where taken from lacZ gene of Escherichia coli.
The GFP BBa_E0040 was taken from the already registered part.
This is a composite part that consists in our Minimum Blue Light Receptor Promoter (BBa_K360041) with our Strong RBS with spacer (BBa_K360031) and GFP BBa_E0040.
false
false
_485_
0
7013
9
It's complicated
true
See design considerations for Minimum Blue Light Receptor Promoter BBa_K360041.
false
Jorge Eduardo Buend??a Buend??a
component2092312
1
BBa_E0040
component2092307
1
BBa_K360041
component2092310
1
BBa_K360031
annotation2092312
1
BBa_E0040
range2092312
1
76
795
annotation2092310
1
BBa_K360031
range2092310
1
59
69
annotation2092307
1
BBa_K360041
range2092307
1
1
50
BBa_K360031
1
BBa_K360031
Strong RBS with spacer.
2010-10-20T11:00:00Z
2015-05-08T01:12:12Z
From lacZ gene of Escherichia coli.
Strong RBS with a seven bases spacer before start codon.
false
false
_485_
0
6838
9
Not in stock
false
It works very well, although the spacer is not the strongest.
false
Jorge Arturo Zepeda Mart??nez
annotation2091558
1
Spacer
range2091558
1
5
11
annotation2091557
1
RBS
range2091557
1
1
4
BBa_K360041
1
BBa_K360041
Minimum Blue Light Receptor Promoter
2010-09-11T11:00:00Z
2015-05-08T01:12:12Z
Synthetized as a primer to insert this minimum blue promoter into a plasmid
This is a short version of the promoter (BBa_K238013) activated by the blue light receptor system YcgF/YcgE, the original promoter was described and characterized by the K.U. Leuven 2009 team. This short promoter includes nucleotides from 26-75 of the original sequence reported by the K.U. Leuven 2009 team.
false
false
_485_
0
7013
9
It's complicated
true
When designing this short version of the blue promoter we considered to include the following elements: -35 box, spacer, -10 box, TSS and inverted repeat 1 and 2
false
Jorge Eduardo Buend??a Buend??a
annotation2079889
1
inverted repeat part 2
range2079889
1
42
48
annotation2079890
1
transcription start site
range2079890
1
38
40
annotation2079886
1
spacer
range2079886
1
9
26
annotation2079885
1
-35 box
range2079885
1
3
8
annotation2079887
1
-10 box
range2079887
1
27
33
annotation2079888
1
inverted repeat part 1
range2079888
1
28
34
BBa_E0040
1
GFP
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
2004-09-29T11:00:00Z
2016-01-26T02:09:38Z
Released HQ 2013
GFP (mut3b) [note that this part does not have a barcode]
false
true
_11_1_
4206
61
7
In stock
false
true
jcbraff
annotation1934520
1
GFP protein
range1934520
1
1
720
BBa_K360041_sequence
1
tgtacacatatttcgtacaagtttgctattgttacttcacttaacattga
BBa_K360031_sequence
1
aggaaacagct
BBa_E0040_sequence
1
atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
BBa_K360141_sequence
1
tgtacacatatttcgtacaagtttgctattgttacttcacttaacattgatactagagaggaaacagcttactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z