BBa_K361002
1
BBa_K361002
Rci recombinase system
2010-10-23T11:00:00Z
2015-05-08T01:12:12Z
The promotor is come from lacI regulated one: BBa_R0010
The ribosome binding site is come from the defines RBS efficiency equal to one : BBa_B0034
The terminator is come from a doble terminator with bidirectional termination: BBa_B0014
This is the protein genertors of Rci site-specific recombinase BBa_K361000, which is being linked with a promotor, a ribosome binding site in front of it, and a double terminator after it.
true
false
_476_
0
6123
9
Discontinued
false
This is the most optimal system for Rci site-specific recombinase production.
false
Jacky, Fong Chuen, Loo
annotation2095212
1
BBa_K361000
range2095212
1
1
1155
annotation2095211
1
Rci recombinase
range2095211
1
1
1155
BBa_K361000
1
Rci
Rci site-specific recombinase
2010-10-23T11:00:00Z
2015-05-08T01:12:12Z
It belongs to integrase family of site-specific recombinase, and it is extracted from muliple inversion systems in R64 plasmid, found in genomic sequence code:AP005147.1
This part will prouce a 384-amino acid Rci site-specific recombinase used in shufflon system, which is a site-specific DNA recombination between two specific repeat sites as recognition.
After this recombinase is being expressed, it can alter the DNA sequence being inserted randomly with the specific repeat sites originally to become an unknown sequence.
false
true
_476_
0
6123
9
It's complicated
true
The gene is come from muliple inversion systems in R64 plasmid and there is no further modification.
Reports have shown that some modification of the gene will lead to deletion some parts of DNA sequence during recombination process.
false
Jacky, Fong Chuen, Loo
annotation2095181
1
Rci recombinase
range2095181
1
1
1155
BBa_K361002_sequence
1
atgccgtctccacgcatccgtaaaatgtccctgtcacgcgcactggataagtacctgaaaacagtttctgttcacaagaaagggcatcaacaggagttttaccggagcaatgttatcaagcgatatcccattgcccttcggaatatggacgaaataacaaccgttgatattgctacatacagagacgttcgtttagcagaaatcaacccccgaacgggtaaacccattacaggtaatactgtacgtcttgaactcgcccttctgtcatctctgttcaatattgctcgtgttgaatggggaacctgtcgtactaacccggttgaactggttcgcaagccgaaagtatcctcgggacgagatcgccggctaacgtcttcagaagaacgtcgcctttctcgctatttccgcgaaaaaaatctgatgttgtatgtcattttccatcttgcccttgagacagccatgcggcagggcgaaatactggccttacgttgggagcacattgatttgcgccacggtgtggctcatttacctgaaaccaaaaacggtcactcacgggatgttcctctgtccagacgtgcccgtaactttcttcagatgatgcccgttaatctccacggcaatgtttttgattacaccgcatccggctttaaaaatgcctggagaatagccacacaacgacttcgcatcgaggacctgcattttcacgatctacggcatgaagcaataagccgcttcttcgaactgggtagcctgaatgtaatggagattgctgcaatatcaggacatcgttccatgaatatgctgaaacggtatactcatcttcgtgcatggcaactggtcagtaagcttgatgcccgccggcggcagacacaaaaagtggcagcatggtttgtgccgtatcctgcccatatcacgaccatcgatgaagaaaatgggcagaaagcgcatcgtattgagatcggtgattttgataaccttcacgtcactgccacaacgaaagaggaagcagttcaccgcgccagtgaggttttgttgcgtacactggccattgcagcacagaaaggcgaacgtgtcccatctcccggagcgttacctgttaacgaccctgattacattatgatttgccctctgaacccgggcagcaccccgctgtaa
BBa_K361000_sequence
1
atgccgtctccacgcatccgtaaaatgtccctgtcacgcgcactggataagtacctgaaaacagtttctgttcacaagaaagggcatcaacaggagttttaccggagcaatgttatcaagcgatatcccattgcccttcggaatatggacgaaataacaaccgttgatattgctacatacagagacgttcgtttagcagaaatcaacccccgaacgggtaaacccattacaggtaatactgtacgtcttgaactcgcccttctgtcatctctgttcaatattgctcgtgttgaatggggaacctgtcgtactaacccggttgaactggttcgcaagccgaaagtatcctcgggacgagatcgccggctaacgtcttcagaagaacgtcgcctttctcgctatttccgcgaaaaaaatctgatgttgtatgtcattttccatcttgcccttgagacagccatgcggcagggcgaaatactggccttacgttgggagcacattgatttgcgccacggtgtggctcatttacctgaaaccaaaaacggtcactcacgggatgttcctctgtccagacgtgcccgtaactttcttcagatgatgcccgttaatctccacggcaatgtttttgattacaccgcatccggctttaaaaatgcctggagaatagccacacaacgacttcgcatcgaggacctgcattttcacgatctacggcatgaagcaataagccgcttcttcgaactgggtagcctgaatgtaatggagattgctgcaatatcaggacatcgttccatgaatatgctgaaacggtatactcatcttcgtgcatggcaactggtcagtaagcttgatgcccgccggcggcagacacaaaaagtggcagcatggtttgtgccgtatcctgcccatatcacgaccatcgatgaagaaaatgggcagaaagcgcatcgtattgagatcggtgattttgataaccttcacgtcactgccacaacgaaagaggaagcagttcaccgcgccagtgaggttttgttgcgtacactggccattgcagcacagaaaggcgaacgtgtcccatctcccggagcgttacctgttaacgaccctgattacattatgatttgccctctgaacccgggcagcaccccgctgtaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z