BBa_K361002 1 BBa_K361002 Rci recombinase system 2010-10-23T11:00:00Z 2015-05-08T01:12:12Z The promotor is come from lacI regulated one: BBa_R0010 The ribosome binding site is come from the defines RBS efficiency equal to one : BBa_B0034 The terminator is come from a doble terminator with bidirectional termination: BBa_B0014 This is the protein genertors of Rci site-specific recombinase BBa_K361000, which is being linked with a promotor, a ribosome binding site in front of it, and a double terminator after it. true false _476_ 0 6123 9 Discontinued false This is the most optimal system for Rci site-specific recombinase production. false Jacky, Fong Chuen, Loo annotation2095212 1 BBa_K361000 range2095212 1 1 1155 annotation2095211 1 Rci recombinase range2095211 1 1 1155 BBa_K361000 1 Rci Rci site-specific recombinase 2010-10-23T11:00:00Z 2015-05-08T01:12:12Z It belongs to integrase family of site-specific recombinase, and it is extracted from muliple inversion systems in R64 plasmid, found in genomic sequence code:AP005147.1 This part will prouce a 384-amino acid Rci site-specific recombinase used in shufflon system, which is a site-specific DNA recombination between two specific repeat sites as recognition. After this recombinase is being expressed, it can alter the DNA sequence being inserted randomly with the specific repeat sites originally to become an unknown sequence. false true _476_ 0 6123 9 It's complicated true The gene is come from muliple inversion systems in R64 plasmid and there is no further modification. Reports have shown that some modification of the gene will lead to deletion some parts of DNA sequence during recombination process. false Jacky, Fong Chuen, Loo annotation2095181 1 Rci recombinase range2095181 1 1 1155 BBa_K361002_sequence 1 atgccgtctccacgcatccgtaaaatgtccctgtcacgcgcactggataagtacctgaaaacagtttctgttcacaagaaagggcatcaacaggagttttaccggagcaatgttatcaagcgatatcccattgcccttcggaatatggacgaaataacaaccgttgatattgctacatacagagacgttcgtttagcagaaatcaacccccgaacgggtaaacccattacaggtaatactgtacgtcttgaactcgcccttctgtcatctctgttcaatattgctcgtgttgaatggggaacctgtcgtactaacccggttgaactggttcgcaagccgaaagtatcctcgggacgagatcgccggctaacgtcttcagaagaacgtcgcctttctcgctatttccgcgaaaaaaatctgatgttgtatgtcattttccatcttgcccttgagacagccatgcggcagggcgaaatactggccttacgttgggagcacattgatttgcgccacggtgtggctcatttacctgaaaccaaaaacggtcactcacgggatgttcctctgtccagacgtgcccgtaactttcttcagatgatgcccgttaatctccacggcaatgtttttgattacaccgcatccggctttaaaaatgcctggagaatagccacacaacgacttcgcatcgaggacctgcattttcacgatctacggcatgaagcaataagccgcttcttcgaactgggtagcctgaatgtaatggagattgctgcaatatcaggacatcgttccatgaatatgctgaaacggtatactcatcttcgtgcatggcaactggtcagtaagcttgatgcccgccggcggcagacacaaaaagtggcagcatggtttgtgccgtatcctgcccatatcacgaccatcgatgaagaaaatgggcagaaagcgcatcgtattgagatcggtgattttgataaccttcacgtcactgccacaacgaaagaggaagcagttcaccgcgccagtgaggttttgttgcgtacactggccattgcagcacagaaaggcgaacgtgtcccatctcccggagcgttacctgttaacgaccctgattacattatgatttgccctctgaacccgggcagcaccccgctgtaa BBa_K361000_sequence 1 atgccgtctccacgcatccgtaaaatgtccctgtcacgcgcactggataagtacctgaaaacagtttctgttcacaagaaagggcatcaacaggagttttaccggagcaatgttatcaagcgatatcccattgcccttcggaatatggacgaaataacaaccgttgatattgctacatacagagacgttcgtttagcagaaatcaacccccgaacgggtaaacccattacaggtaatactgtacgtcttgaactcgcccttctgtcatctctgttcaatattgctcgtgttgaatggggaacctgtcgtactaacccggttgaactggttcgcaagccgaaagtatcctcgggacgagatcgccggctaacgtcttcagaagaacgtcgcctttctcgctatttccgcgaaaaaaatctgatgttgtatgtcattttccatcttgcccttgagacagccatgcggcagggcgaaatactggccttacgttgggagcacattgatttgcgccacggtgtggctcatttacctgaaaccaaaaacggtcactcacgggatgttcctctgtccagacgtgcccgtaactttcttcagatgatgcccgttaatctccacggcaatgtttttgattacaccgcatccggctttaaaaatgcctggagaatagccacacaacgacttcgcatcgaggacctgcattttcacgatctacggcatgaagcaataagccgcttcttcgaactgggtagcctgaatgtaatggagattgctgcaatatcaggacatcgttccatgaatatgctgaaacggtatactcatcttcgtgcatggcaactggtcagtaagcttgatgcccgccggcggcagacacaaaaagtggcagcatggtttgtgccgtatcctgcccatatcacgaccatcgatgaagaaaatgggcagaaagcgcatcgtattgagatcggtgattttgataaccttcacgtcactgccacaacgaaagaggaagcagttcaccgcgccagtgaggttttgttgcgtacactggccattgcagcacagaaaggcgaacgtgtcccatctcccggagcgttacctgttaacgaccctgattacattatgatttgccctctgaacccgggcagcaccccgctgtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z